Article
Nanoscience & Nanotechnology
Lei Liu, Mengshu Kong, Youqiang Xing, Ze Wu, Yunfei Chen
Summary: In this study, ReS2 nanotubes and MoS2-ReS2 heterojunction nanotubes were successfully fabricated for the first time using a bimetallic co-chamber feeding atomic layer deposition technique. These nanotubes demonstrated controllable diameter, wall thickness, and desired Mo-to-Re ratio, and their electron-transfer capacity and photocurrent response were effectively enhanced by the incorporated Mo atoms. Among them, MoS2-ReS2-HNTs with a real Mo-to-Re ratio of 31.0% exhibited the best photocurrent response performance, enabling ultrasensitive detection of cancer-related miRNA-155.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Songlong Jiao, Lei Liu, Youqiang Xing, Ze Wu, Bingjue Li, Peng Huang
Summary: The development of photoactive materials based on single-atom catalysts is crucial for improving the performance of PEC biosensors. By designing Pt atoms-anchored MoS2 nanotubes (PtMoS2NT), this study demonstrates the advantages of single-atom catalysts in achieving ultra-sensitive detection of miRNA-155.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Jianhua Yan, Kaidi Wang, Hongjie Liu, Liwei Wang, Yixiang Li, Guoqing Zhang, Li Deng
Summary: In this study, an electrochemical biosensor was developed based on the flower-like MoSe2@1T-MoS2 heterojunction electrode material and specific RNA recognition probes for rapid detection and quantification of miRNA-155 in breast cancer. The biosensor exhibited a wide detection range of 1 fM to 1 nM with a low limit of detection (LOD) of 0.34 fM. The biosensor accurately measured the miRNA-155 content in blood samples of tumor-bearing mice and normal mice, demonstrating its strong identification ability and potential as a diagnostic marker. A portable sensor platform was also designed for on-site detection simulation, which showed good recovery efficiency and verified the accuracy and reliability of the biosensor compared to the standard detection method RT-qPCR, suggesting its potential for point-of-care testing (POCT) in early breast cancer diagnosis.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Fatemeh Khosravi, Mahdi Rahaie, Mohammad Reza Ghaani, Mostafa Azimzadeh, Ebrahim Mostafavi
Summary: Electrochemical nanobiosensors have revolutionized medical diagnosis with their enhanced sensitivity provided by the utilized nanomaterials. In this study, a novel sensitive electrochemical nanobiosensor for miR-155, a biomarker for early breast cancer detection, was introduced. The nanobiosensor utilized a combination of Fe-Ni@rGO, GQD-Ag, and GNS nanomaterials to expand the surface area and conductivity of the electrode, resulting in high sensitivity and specificity for miR-155 detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Zhiwei Sun, Yao Tong, Li Zhao, Juan Li, Fucheng Gao, Chuanxin Wang, Hui Li, Lutao Du, Yanyan Jiang
Summary: This study presents a photoelectrochemical (PEC) biosensor based on MoS2@Ti3C2 nanohybrid for ultrasensitive detection of miRNAs, with a broad linear detection range, high selectivity, and stability. The biosensor demonstrates the potential for clinical application in accurate detection of exosomal miRNAs concentrations, showing promise for ultrasensitive point-of-care detection of miRNAs.
Article
Chemistry, Analytical
Yong-ping Gao, Ke-jing Huang, Fu-ting Wang, Yang-yang Hou, Lu-di Zhao, Bo-ya Wang, Jing Xu, Honglei Shuai, Guoqiang Li
Summary: A self-powered biosensor (SPB) utilizing a capacitor/enzymatic biofuel cell (EBFC), rolling circle amplification (RCA), and a digital multimeter (DMM) was developed for ultra-sensitive detection of miR-155. The EBFC-SPB exhibited a sensitivity of 15.85 mu A/pM with the matching capacitor, which is 513% higher than without capacitor (3.09 mu A/pM), achieving the first signal amplification. RCA triggered by miR-155 generated continuous electrons that flowed to the biocathode, catalyzing oxygen reduction and achieving the second signal amplification. DMM was used for signal conversion and real-time amplification, resulting in a LOD of 0.17 fM and a linear range of 0.5 fM to 10,000 fM, indicating the broad application prospects of EBFC-SPB for ultra-sensitive detection of miR-155.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Environmental
Sachin A. Pawar, Dipali S. Patil, Dip K. Nandi, Muhammad Monirul Islam, Takeaki Sakurai, Soo-Hyun Kim, Jae Cheol Shin
Summary: In this study, a novel approach of preparing a composite supercapacitor electrode consisting of ternary metal oxide nano-structure decorated with transition metal sulfide is presented. The hybrid structure reveals superior electrochemical performance, and the thinner and well-connected nanosheets of NiCo2O4 provide more interface between the electrode and the electrolyte. The high areal capacitance and enhanced rate capability of the optimized NiCo2O4-MoS2 composite are attributed to the synergistic effect of high-surface area of the oxide nano-sheets and better electronic conductivity of the sulfide.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Microbiology
Kul Raj Rai, Yuan Liao, Mengjuan Cai, Haori Qiu, Faxin Wen, Min Peng, Song Wang, Shasha Liu, Guijie Guo, Xiaojuan Chi, Mohamed Maarouf, Yuhai Chen, Shile Huang, Ji-Long Chen
Summary: In this study, lncRNA-155KO mice lacking most of the lncRNA-155 sequences were found to be more susceptible to influenza virus or pseudorabies virus infection compared to miRNA-155KO mice. The loss of lncRNA-155 resulted in faster body weight loss, poorer survival, and higher viral load. The findings suggest the involvement of lncRNA-155 in regulating viral pathogenesis in addition to miRNA-155. miRNA-155-deleted lncRNA-155 significantly attenuated the viral infection.
Article
Oncology
Da Qin, Qingdong Guo, Rui Wei, Si Liu, Shengtao Zhu, Shutian Zhang, Li Min
Summary: This study proposed a new algorithm to quantitate plasma miRNA for colon cancer diagnosis. By establishing a miRNA-pair matrix and using various algorithms, a 4-miRNA pair model was successfully developed that showed good performance in differentiating CC from normal controls.
FRONTIERS IN ONCOLOGY
(2021)
Review
Immunology
Salar Pashangzadeh, Morteza Motallebnezhad, Fatemeh Vafashoar, Azadeh Khalvandi, Nazanin Mojtabavi
Summary: miRNAs, especially miR-155, play crucial roles in immune system regulation, and their altered expression and function may contribute to vulnerability to autoimmune diseases and significant dysfunction of the immune system.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Urology & Nephrology
Sonat Pinar Kara, Gulsum Ozkan, Ahsen Yilmaz, Nergiz Bayrakci, Savas Guzel, Elif Geyik
Summary: The study revealed significantly higher levels of miRNA 21 and aldosterone in patients with resistant hypertension compared to healthy individuals and newly diagnosed hypertensives.
Article
Oncology
Da Wang, Fei Xiong, Guanhua Wu, Wenzheng Liu, Bing Wang, Yongjun Chen
Summary: The study found that in cholangiocarcinoma, miR-155-5p targets the 3' UTR of SOX1, activating the RAF/MEK/ERK pathway and promoting cancer progression.
CANCER CELL INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Yongjun Bian, Mengwei Li, Zhaojie Wu, Jian Weng, Ru Zeng, Liping Sun
Summary: Researchers have developed an ultrasensitive fluorescent biosensor using sporopollenin capsules as a substrate for isothermal amplification. This biosensor can detect miRNA with a low detection limit and a wide linear range, and it shows high selectivity and minimal interference from serum proteins.
Article
Reproductive Biology
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Gyeonghwan Seong, Mahanama De Zoysa, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: This study investigated the effect of miRNA-155 on parthenogenically activated early development porcine embryos in vitro. miRNA-155 inhibition improved in vitro developmental competence by increasing cell numbers and reducing stress and apoptosis levels. The cleavage rate and blastocyst rate in the miRNA-155 inhibitor group were significantly higher than in the control and miRNA-155 mimic groups.
Article
Biochemistry & Molecular Biology
Rania Elsayed, Salwa Fayez, Laila Ahmed Rashed, Marwa Farghali, Marwa AbdelHamid, Marwa Alkaffas
Summary: This study aimed to investigate the plasma level of miRNA-155 in RRMS patients and its relationship with inflammatory and anti-inflammatory mediators. The results showed no significant difference in miRNA-155 expression between MS patients and controls, but there was a significant increase in TNF-alpha, INF-gamma, and TGF-beta levels. IL-10 levels did not differ significantly between the two groups. Furthermore, miRNA-155 had a positive correlation with TNF-alpha, INF-gamma, and iNOS, and an inverse correlation with IL-10, TGF-beta, and SMAD2.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)