Journal
BIOSENSORS & BIOELECTRONICS
Volume 144, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111626
Keywords
Intracellular action potential recording; Cardiomyocytes; CMOS; Multimodality sensor array; Cellular impedance sensing; Drug screening
Categories
Funding
- NSF CAREER [1454555]
- NHLBI [5F31HL137367-02, 1R01HL111646-01A1]
- NSF [1609831]
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [1454555, 1609831] Funding Source: National Science Foundation
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Intracellular action potential signals reveal enriched physiological information. Patch clamp techniques have been widely used to measure intracellular potential. Despite their high signal fidelity, they suffer from low throughput. Recently, 3D nanoelectrodes have been developed for intracellular potential recording. However, they are limited by scalability, yield, and cost, directly constraining their use in monitoring large number of cells and high throughput applications. In this paper, we demonstrate intracellular potential monitoring of cardio-myocytes using simple 2D planar electrode array in a standard CMOS process without patch clamps or post fabricated 3D nanoelectrodes. This is enabled by our unique cardiomyocytes/fibroblasts co-culturing technique and electroporation. The co-cultured fibroblasts promote tight sealing of cardiomyocytes on electrodes and enable high-fidelity intracellular potential monitoring based on 2D planar electrode. Compared to existing technologies, our platform has a unique potential to achieve an unprecedented combination of throughput, spatiotemporal resolution, and a tissue-level field-of-view for cellular electrophysiology monitoring.
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