Journal
BIOSENSORS & BIOELECTRONICS
Volume 142, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111579
Keywords
Photoelectrochemical biosensor; DNA detection; Fullerene; Methylene blue; Sensitizer
Categories
Funding
- National Natural Science Foundation of China [21575116, 21775124, 21675129]
- Fundamental Research Funds for the Central Universities [XDJK2018AA003]
Ask authors/readers for more resources
In this protocol, a photoelectrochemical (PEC) biosensor based on methylene blue (MB) sensitized fullerene (C-60) was designed for ultrasensitive DNA detection. First, C-60 nanoparticles (C-60 NPs) as photoactive material was modified on the electrode surface to obtain an initial photocurrent signal. Additionally, the nicking endonuclease (Nb.BvCI enzyme)-assisted signal amplification strategy was implemented to transform limited target to plenty of products, which hybridized with DNA2 to form plentiful DNA duplex for immobilizing numerous MB as the sensitizer, thus accomplishing the effective sensitization toward C-60 NPs and achieving an enhanced photo-current for quantitatively detecting target DNA. This established PEC strategy realized the target DNA detection with the detection limit down to 3.3 fM, which paved a new avenue for DNA determination and exhibited huge application potential for other biomarkers in clinical analysis and disease diagnosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available