Article
Chemistry, Analytical
Zhou-Jie Liu, Liang-Yong Yang, Tai-Cheng Lu, Yu-Qi Liang, Meng-Meng Liu, Guang-Xian Zhong, Xin-Hua Lin, Pin-Fang Huang, Jin-Yuan Chen
Summary: This study presents a lambda exonuclease-assisted ligase chain reaction-based electrochemical biosensor, named lambda-eLCR, for highly sensitive and accurate genotyping of the CYP2C19*2 allele. The biosensor features a methylene blue-attached DNA probe and an inverted hybridization format, enabling zero-background analysis. The lambda-eLCR exhibited excellent performance in terms of a good linear range and low limit of detection, and successfully distinguished the CYP2C19*2 allele genotypes in human whole blood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Shengqiang Li, Zhengxiang Fu, Chao Wang, Xipeng Shang, Yan Zhao, Cuiying Liu, Ming Pei
Summary: The electrochemical biosensor developed for DNA determination utilizes T7 exonuclease-assisted regulatory strand displacement dual recycling signal amplification strategy, showing high sensitivity and selectivity. It has been successfully applied for target DNA detection in serum, demonstrating potential for molecular diagnostics.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Luxia Yang, Xiaobo Zhou, Ke Zhang, Jinxia Liu, Lingfeng Zhao, Aiting Cai, Xinyuan Zhao, Li Wu, Yuling Qin
Summary: In this study, an electrochemiluminescent (ECL)/electrochemical (EC) ratiometric biosensor was developed for ultrasensitive determination of circulating tumor DNA (ctDNA) using an exonuclease III (Exo III)-assisted target recycling strategy. The ratiometric biosensing was achieved by integrating the electrochemical activity and ECL quenching property of ferrocene (Fc). Additionally, a novel aggregation-induced electrochemiluminescence (AIECL) molecule, named 4,4'-(benzo[c][1,2,5]thiadiazole-4,7-diyl)bis(N, N-diphenylaniline) (TPA-BT), was designed and synthesized to enhance the AIECL emission, enabling the construction of the ratiometric biosensor. The optimized biosensor exhibited a wide detection range (100 aM-100 pM) and a low detection limit (11.2 aM) for ctDNA analysis (KRAS gene), with potential for multiplexed detection of other ctDNAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ping Ouyang, Chenxin Fang, Jialun Han, Jingjing Zhang, Yuxing Yang, Yang Qing, Yubing Chen, Wenyan Shang, Jie Du
Summary: The study introduces an electrochemical DNA biosensor for protein detection based on DNA self-assembly and terminal protecting effects of small-molecule-linked DNA. This strategy allows for qualitative and quantitative analysis of target proteins with high sensitivity in complex samples.
Article
Chemistry, Analytical
Di Zhang, Xin Yu, Lina Wu, Huali Jin, Min Wei
Summary: A sensitive and selective electrochemical Pb2+ sensor was developed based on DNAzyme and exonuclease III, allowing for a wide detection range and high sensitivity in detecting Pb2+ concentrations through target recycling amplification.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zaofen Wang, Weiping Shi, Yunzhu Tan, Bingqian Liu
Summary: A novel label-free homogeneous electrochemical aptamer sensor for the detection of progesterone was developed by combining omega-like DNA nanostructure with an isothermal cycling amplification strategy based on exonuclease III. The sensor exhibits high sensitivity and a wide dynamic linear range.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Zhen Song, Rong Li, Xiqin Yang, Zhanhua Zhang, Xiliang Luo
Summary: This study developed an electrochemical biosensing platform with enhanced antifouling abilities by immobilizing DNA-peptide conjugates onto an electrode. The biosensor exhibited enhanced antifouling properties and was capable of detecting low fouling proteins directly in complex biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Zhigang Tang, Wenshu Zhang, Yalun Hu, Xiaodan Jia, Lina Meng, Dan Wang, Lijuan Chen, Junwei Zhao
Summary: An unprecedented organophosphonic acid and cerium functionalized antimonotungstate was synthesized, and a ternary composite material containing reduced graphene oxide and gold nanoparticles was prepared, which exhibited remarkable electrical conductivity and long-term stability. The composite material was then used as an electrode modification material to construct an electrochemical DNA biosensor for detecting the bacterial dissimilatory sulfite reductase gene. The biosensor showed outstanding sensitivity, selectivity, and anti-interference capability.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Analytical
Wenting Cheng, Jiehua Ma, Qinfang Tao, Khan Adeel, Liangliang Xiang, Duxian Liu, Zhaoli Zhang, Jinlong Li
Summary: This study developed a new rapid and sensitive quantitative method to analyze the activity of ALKBH3 using an electrochemical approach. The detection of ALKBH3 activity was achieved by monitoring the changes in electrochemical signal, providing a new method for clinical detection of prostate cancer.
Article
Chemistry, Analytical
Siling Chen, Rongjun Yu, Ying Li, Jiangling Wu, Jingfu Qiu, Xinyi Huang, Jianjiang Xue
Summary: In this study, a new biosensor was developed for the sensitive detection of the CYP2C19*2 gene. The biosensor utilized exonuclease III assisted electric signal amplification and calixarene for the enrichment of signal substances. The method demonstrated good selectivity, high sensitivity, and stability, making it a promising tool for the detection of the CYP2C19*2 gene and other biological molecules.
Article
Biotechnology & Applied Microbiology
Imen Tanniche, Amanda K. Fisher, Frank Gillam, Eva Collakova, Chenming Zhang, David R. Bevan, Ryan S. Senger
Summary: Lambda-PCR is an effective method for DNA assembly and cloning, which utilizes overlap extension and different variants to achieve stable, efficient, and cost-effective assembly of DNA sequences.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Immunology
Joelle R. Madi, Amani Al Outa, Mirna Ghannam, Hadi M. Hussein, Marwa Shehab, Zeinab Al Kobra Haj Hasan, Antoine Abou Fayad, Margret Shirinian, Elias A. Rahal
Summary: The study suggests that EBV DNA may worsen intestinal inflammation by enhancing the IMD pathway and increasing cellular inflammatory responses, potentially perpetuating proinflammatory processes in an inflamed digestive system. D. melanogaster can serve as a model to further understand EBV-associated gastroinflammatory pathologies and further studies in mammalian models may validate these findings.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zhaohui Qin, Yu Liu, Linghao Zhang, Jiajia Liu, Xin Su
Summary: This study demonstrates a DNA-based artificial dissipative system with a molecular timer, which allows for dynamic enzyme catalysis and DNA nanostructure disassembly. This provides a useful tool for the development of artificial reaction networks and intelligent nanostructures.
Article
Chemistry, Analytical
Qianqian Cai, Hongkun Li, Wei Cao, Guifen Jie
Summary: A new fluorescence biosensor platform based on distance-dependent PET and target cross-chain displacement cyclic amplification strategy was developed to detect MicroRNA. The platform can be applied to quantitative determination of miRNA-182-5p, showing great potential in early clinical diagnosis of miRNA related diseases.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Qirong Chen, Huahui Gao, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a new target/aptamer binding-induced inhibition of enzyme activity strategy was used to construct an amplified and sensitive electrochemical biosensor for detecting Sonic Hedgehog (SHh). The sensor exhibited high selectivity and could detect SHh down to 3.34 pM. This strategy has the potential to be a convenient approach for detecting different proteins in biological studies and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)