Article
Chemistry, Analytical
Yuting Zhang, Hui Li, Zongkang Guo, Xiaoli Wang, Nandi Zhou
Summary: Electrochemical aptasensors have limitations due to variations in aptamer loading densities and low signal amplification efficiency. In this study, an immobilization-free and label-free electrochemical homogeneous aptasensor was developed for CEA assay using RecJf exonuclease-mediated target cycling strategy and rolling circle amplification technology. This system eliminates the need for pre-immobilization on electrode substrates and shows good versatility and stability, as well as improved sensitivity in the homogeneous solution phase. The proposed aptasensors achieve satisfactory results in actual sample tests and have potential for commercial applications and future target platforms.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Soraya Mozafari Vanani, Zahra Izadi, Roohullah Hemmati, Behnaz Saffar
Summary: This study developed a sensitive electrochemical aptasensor for the detection of streptomycin residues in animal products. The aptasensor demonstrated high sensitivity, selectivity, and stability, allowing for rapid and accurate detection of trace amounts of streptomycin in dairy products.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Environmental Sciences
Kai Wang, Han Yan, Baoshan He, Lingling Xie, Renli Liu, Min Wei, Huali Jin, Wenjie Ren, Zhiguang Suo, Yiwei Xu
Summary: A label-free aptasensor using cerium-based metal-organic framework composite gold nanoparticles was developed for detecting vomitoxin residues in grains. The aptasensor achieved the separation of the DON-aptamer complex and cDNA using magnetic separation technique with magnetic beads, ensuring specificity. Exonuclease III-assisted cDNA cycling process strategy was triggered for signal amplification. The aptasensor showed a wide detection range and a low detection limit, indicating its reliability and potential in DON detection.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Kaiyan Fan, Junlun Zhu, Xiaowei Wu, Xiuhua Zhang, Shengfu Wang, Wei Wen
Summary: A flexible and label-free electrochemical biosensing platform based on split aptamers and exonuclease I had been developed for parallel detection of different targets. The label-free aptasensor achieved sensitive and selective detection of thrombin, adenosine triphosphate, and mercury ion.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Baozhong Zhang, Xinyue Ma, Lingling Xie, Xiquan Li, Lingyun Chen, Baoshan He
Summary: An ultrasensitive electrochemical aptasensor was developed in this study for the detection of tobramycin (TOB) residues. The sensor utilizes a signal amplification strategy and provides a broad linear range with a low detection limit. It exhibits favorable stability, specificity, and reproducibility, and has been successfully applied to practical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Haishuai Peng, Yuanyuan Hui, Lei Zhang, Fuxin Zhang, Yufang Liu, Jianbin Zheng, Rong Jia, Yuxuan Song, Bini Wang
Summary: In this study, an electrochemical aptasensor based on two-dimensional titanium carbide and metal-organic frameworks nanomaterials was developed for the rapid detection of streptomycin residue in milk samples. The aptasensor exhibited wide detection range, low limit of detection, excellent reproducibility, stability, and selectivity. The proposed method provides a new and effective approach for the rapid detection of streptomycin and a sensing platform for the detection of other toxins.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Applied
Haishuai Peng, Yuanyuan Hui, Meixue Pu, Ding Yang, Aiqing Zhao, Weizhe Wang, Shan Wu, Bini Wang
Summary: In this study, a novel electrochemical aptasensor was developed for the rapid detection of streptomycin residues in dairy products. The method is simple, fast, and sensitive, and showed satisfactory results in the detection of streptomycin in dairy products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Lixia Yang, Zi Yi, Xike Zeng, Xiaobei Huang, Feifei Zhong, Jinsha Zhou, Zhipeng Qiu
Summary: The method combines RecJ(f) exonuclease with graphdiyne to achieve highly sensitive and selective detection of chloramphenicol, which is of great significance for the detection of chloramphenicol in food samples.
ANALYTICAL LETTERS
(2022)
Article
Chemistry, Analytical
Bingnan Deng, Zhicong Ni, Wenhao Yang, Jiyue Hou, Rongwei Huang, Xue Li, Yiyong Zhang
Summary: This paper presents a method for growing three-dimensional nanometer reticular TiO2 (r-TiO2) on a Ti substrate using the hydrothermal method, and then depositing high-conductivity and high-capacity Sn metal on the r-TiO2 structure using electroless plating modification technology. The resulting r-Sn/TiO2 composite electrode shows enhanced specific surface area, increased specific capacity, and excellent rate capability, making it a promising material for high energy density and high current charging lithium-ion batteries.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Dong Liu, Shuyun Meng, Xiuli Shen, Yuye Li, Xiaohong Yan, Tianyan You
Summary: This work presents a dual-ratiometric aptasensor for streptomycin detection, utilizing the in-situ coupling of photoelectrochemical and electrochemical assays. The use of methylene blue as a bifunctional probe enables the simultaneous generation of photocurrent and redox current, resulting in enhanced analytical performances compared to single-signal or single-ratiometric assays. The MB-based dual-mode sensing offers a promising tool for studying interactions between PEC and EC behaviors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Xiaoyu Su, Xiaoyu Tian, Zongbao Sun, Xiaobo Zou, Wen Zhang
Summary: This work introduces a signal-on electrochemical aptasensor for sensitive Hg2+ determination in shellfish, based on RGO-AuNPs and exonuclease-III (Exo-III). RGO-AuNPs is electrodeposited on a screenprinted electrode (SPE) as a high-performance sensing platform. The sensor shows good linear relationship and low detection limit (LOD) for Hg2+ detection in shellfish samples, with errors and recovery rates within acceptable ranges.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Min Wei, Lingkun Xin, Huali Jin, Yawei Huang, Yong Liu
Summary: A sensitive electrochemical aptasensor was developed to detect zearalenone, with a wide linear range and low detection limit, showing high specificity, repeatability, and reproducibility for aflatoxin B1 (AFB1), fumonisin B1 (FB1), and ochratoxin A (OTA). The method has significant implications for food safety detection.
Article
Chemistry, Analytical
Baoshan He, Senyao Wang
Summary: The electrochemical aptasensor for CAP detection is based on PEI-C3N4/AuNWs and exonuclease-assistant signal amplification, resulting in a linear relationship and low detection limit, with excellent specificity, reproducibility, and long-term stability.
Article
Chemistry, Analytical
Haining Cui, Keqi An, Chengquan Wang, Yangfang Chen, Suli Jia, Jing Qian, Nan Hao, Jie Wei, Kun Wang
Summary: A disposable ratiometric electrochemical aptasensor was constructed for highly sensitive detection of aflatoxin B1. Au nanoflowers on ITO surface were used for DNA anchoring and methylene blue labeling. The detection was based on the peak current intensity ratio (I-MB/I-Fc) as the signal transducer. The aptasensor exhibited a wide detection range and a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Xia Lu, Baoshan He, Ying Liang, Jinshui Wang, Qiang Jiao, Yong Liu, Rui Guo, Min Wei, Huali Jin, Wenjie Ren, Zhiguang Suo
Summary: In this study, a dual-enzymes-driven electrochemical aptasensor was constructed for ultra-sensitive detection of patulin (PAT) in fruits. The aptasensor showed favorable selectivity, reproducibility, and long-term stability. In real sample analysis, the aptasensor demonstrated good accuracy and precision.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)