Article
Engineering, Biomedical
Pengyue You, Yuhua Liu, Xinzhi Wang, Bowen Li, Weiyi Wu, Lin Tang
Summary: This study investigated the potential application of natural acellular porcine pericardium (APP) as a barrier membrane for guided bone regeneration (GBR). The results showed that APP exhibited osteoconductive and osteoinductive properties, promoting bone regeneration in a critical-sized calvarial defect model compared to collagen membrane and control group. Overall, APP demonstrated promising capabilities for GBR in terms of biomaterial properties and bone healing effects.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Engineering, Biomedical
Anne E. Faust, Lorenzo Soletti, Nicole A. Cwalina, Andrew D. Miller, Matthew D. Wood, Mark A. Mahan, Jonathan Cheetham, Bryan N. Brown
Summary: This study describes the development and testing of a xenogeneic nerve cap graft made from decellularized porcine nerve. The grafts showed promising results in in vitro and in vivo testing, providing proof of concept for a novel xenograft-based approach to neuroma prevention.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Engineering, Biomedical
Ananya Pattnaik, A. Swaroop Sanket, Sanghamitra Pradhan, Rajashree Sahoo, Sudiptee Das, Swarnaprbha Pany, Timothy E. L. Douglas, Rambabu Dandela, Qiang Liu, Jaykumar Rajadas, Sanghamitra Pati, Stefaan C. Smedt, Kevin Braeckmans, Sangram Keshari Samal
Summary: Gradient scaffolds are three-dimensional structures that mimic the biological extracellular matrix with gradual transitions in various properties. They have shown advantages in nutrient and cell penetration, cellular adhesion, viability and differentiation, mechanical stability, and biocompatibility compared to conventional scaffolds. This review explains recent advancements in designing gradient scaffolds and their implications in tissue regeneration applications, as well as discussing the specific utility of gradients in different native tissues.
Review
Biochemistry & Molecular Biology
Alexey A. Ivanov, Alla V. Kuznetsova, Olga P. Popova, Tamara I. Danilova, Oleg O. Yanushevich
Summary: Cell-free therapy has rapidly developed in regenerative medicine over the past decade, aiming to control and enhance tissue repair processes by understanding molecular mechanisms and signaling pathways. The concept of endogenous regenerative medicine, involving the recruitment/homing of endogenous stem cells, has advantages in overcoming limitations and risks associated with cell therapy. Cell-free products such as decellularized extracellular matrix, growth factors, extracellular vesicles, and miRNAs show potential in endogenous bone and dental regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Abdel H. Mahmoud, Yuanyuan Han, Renan Dal-Fabbro, Arwa Daghrery, Jinping Xu, Darnell Kaigler, Sarit B. Bhaduri, Jos Malda, Marco C. Bottino
Summary: In this study, highly innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes were fabricated via electrospinning for guided bone regeneration (GBR). The composite microfibers showed a uniform porous network and successful integration of β-TCP particles. The GelMA/PCL-TCP membranes exhibited enhanced cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs) compared to pure PCL and GelMA/PCL. Furthermore, the GelMA/PCL-TCP membrane promoted robust bone regeneration in rat calvarial critical-size defects, demonstrating remarkable osteogenesis compared to PCL and GelMA/PCL groups. Overall, the electrospun GelMA/PCL-TCP composite showed great potential as a promising membrane for guided bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Tianhao Yu, Qiang Ao, Tianrang Ao, Muhammad Arslan Ahmad, Aijun Wang, Yingxi Xu, Zhongti Zhang, Qing Zhou
Summary: This study developed an optimized multichannel acellular nerve allograft (ANA) with improved ultrastructure and mechanical properties. The ANAs retained more extracellular matrix (ECM) components and bioactive molecules, which enhanced their regenerative capacity and biocompatibility. Both in vitro and in vivo results showed that the optimized ANAs had significant advantages in promoting nerve regeneration and functional recovery.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Jiali Luo, Jing Cao, Guangrong Ma, Xue Wang, Yu Sun, Cong Zhang, Zhan Shi, Yiqing Zeng, Tao Zhang, Pintong Huang
Summary: In this study, collagenase-loaded hollow TiO2 nanoparticles were synthesized to degrade stromal barriers in pancreatic cancer and generate reactive oxygen species. These nanoparticles effectively reduced intratumoral pressure and enhanced the penetration and retention within tumor tissues. Moreover, they improved intratumoral ultrasound signal and provided a highly effective sonodynamic therapy for pancreatic cancer.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Jiali Luo, Jing Cao, Guangrong Ma, Xue Wang, Yu Sun, Cong Zhang, Zhan Shi, Yiqing Zeng, Tao Zhang, Pintong Huang
Summary: This study synthesized collagenase-loaded hollow TiO2 nanoparticles capable of degrading stromal barriers and generating sufficient reactive oxygen species in pancreatic cancer. It showed improved sonodynamic therapy efficacy by decreasing interstitial fluid pressure, enhancing nanoparticle penetration, and providing ultrasound imaging-guided treatment.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Xia Jiang, Xianze Xiong, Yixin Lin, Yanrong Lu, Jingqiu Cheng, Nansheng Cheng, Jie Zhang
Summary: This study developed an artificial bile duct (ABD) for bile duct regeneration, using biodegradable polyurethane and ureter acellular matrix to construct the ABD with favorable biocompatibility and prevention of bile leakage. The mechanical properties, bile flow simulation, and cytocompatibility of the ABD were evaluated in vitro, and in vivo implantation in minipigs demonstrated the degradation of the ABD and the regeneration of the new bile duct. The results showed that the ABD possessed excellent properties for bile duct regeneration, making it a promising material for further application study.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Sadia Perveen, Daniela Rossin, Emanuela Vitale, Rachele Rosso, Roberto Vanni, Caterina Cristallini, Raffaella Rastaldo, Claudia Giachino
Summary: Myocardial infarction is a leading cause of heart-related deaths worldwide, and the potential of acellular scaffolds in treating MI is significant, as they can improve cardiac regeneration, limit adverse LV remodelling, and enhance heart function recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Reut Shainer, Vardit Kram, Tina M. Kilts, Li Li, Andrew D. Doyle, Inbal Shainer, Daniel Martin, Carl G. Simon, Jinyang Zeng-Brouwers, Liliana Schaefer, Marian F. Young, Genomics and Computational Biol Core
Summary: Biglycan is a small proteoglycan found in the extracellular matrix and plays an important role in bone development. Our study reveals that biglycan is involved in osteoblast maturation during embryonic development, affecting bone integrity and strength. Deletion of the biglycan gene reduces the inflammatory response after fracture, leading to impaired periosteal expansion and callus formation. Additionally, biglycan is crucial for the cartilage phase preceding bone formation, as its absence leads to accelerated bone development with high levels of osteopontin, which negatively impacts bone structural integrity.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yiwei Zhu, Qi Zhang, Shengyu Wang, Jianfeng Zhang, Shunwu Fan, Xianfeng Lin
Summary: Decellularized plant scaffolds have advantages such as good biocompatibility, low cost, and no ethical or supply constraints, but there are still issues like immature standards and methods, as well as insufficient research on biocompatibility. Research in this field is still in its early stages and requires further improvement and study.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Biomaterials
Jingjing Wu, Mengyu Yao, Yonggang Zhang, Zefeng Lin, Wenwu Zou, Jiaping Li, Pamela Habibovic, Chang Du
Summary: Distinct structural properties and osteogenic capacity are important for developing guided bone regeneration membranes. The three-layered membranes designed in this study demonstrated good integration, tensile properties similar to natural periosteum, and supported attachment, proliferation, ingrowth, and osteogenic differentiation of human bone marrow-derived stromal cells.
REGENERATIVE BIOMATERIALS
(2021)
Article
Engineering, Biomedical
Bin Wang, Qinglai Tang, Qian Yang, Mengmeng Li, Shiying Zeng, Xinming Yang, Zian Xiao, Xinying Tong, Lanjie Lei, Shisheng Li
Summary: Due to their unique properties such as low immunogenicity, biomimetic internal environment, tissue-and organ-like physicochemical properties, and functionalization potential, decellularized extracellular matrix (dECM) materials have gained considerable attention in tissue engineering. This review provides an overview of the composition of extracellular matrices and their role in stem-cell differentiation, discusses the advantages and disadvantages of existing decellularization techniques, and presents methods for the functionalization and characterization of decellularized scaffolds. Additionally, the review discusses the progress and shortcomings of using dECMs in cartilage, skin, nerve, and muscle repair, as well as the transplantation or regeneration of different whole organs, and examines the future prospects of refunctionalization in tissue and organ repair. It aims to systematize the application of functionalized dECMs in tissue/organ transplantation and keep researchers informed about the latest progress in dECM usage.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
My Thi Ngoc Nguyen, Ha Le Bao Tran
Summary: The study aimed to prepare injectable acellular adipose matrix (AAM) through delipidating and decellularizing, showing that mechanical disruption improved fat removal efficiency and the decellularization strategy was effective. AAM powder was non-toxic to human adipose derived stem cells and supported cell migration and proliferation.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2021)
Article
Engineering, Biomedical
Yang Liu, Lu Yu, Di Zhang, Xiaoning He, Rabia Javed, Qiang Ao
Summary: Alveolar ridge augmentation can use various bone graft materials, including autogenous bone, allograft, xenograft, and alloplastic material. ATR, an acellular tooth root, shows good biocompatibility and can be used as a new tooth-derived bone graft material for alveolar ridge augmentation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biochemistry & Molecular Biology
Muhammad Arslan Ahmad, Xu Deng, Muhammad Adeel, Muhammad Rizwan, Noman Shakoor, Yuesuo Yang, Rabia Javed
Summary: This study demonstrates an increase in secondary metabolites in Stevia rebaudiana plants after the removal of Ca and Mg from the culture medium. Nutrient stress on regenerants of S. rebaudiana leads to enhanced growth parameters and the production of defensive secondary metabolites by generating reactive oxygen species.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Cell Biology
Xiaohong Tian, Wumei Wei, Yue Cao, Tianrang Ao, Feng Huang, Rabia Javed, Xiaohong Wang, Jun Fan, Yanhui Zhang, Yanying Liu, Laijun Lai, Qiang Ao
Summary: GMSC-Exo shows potential advantages in treating rheumatoid arthritis by reducing incidences and bone erosion of arthritis through immunomodulation, bypassing potential shortcomings of direct use of GMSC.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Environmental Sciences
Xiu Liu, Hekai Yang, Xinzhu Yan, Shuangfeng Xu, Yonggang Fan, He Xu, Yue Ma, Weijian Hou, Rabia Javed, Yanhui Zhang
Summary: This study investigated the effects of co-exposure to iron and microplastics on cognitive function in aged humans and animals. The findings showed that co-exposure exacerbated iron overload, cognitive deficits, and increased lipid peroxidation and inflammation in the brain.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Biochemical Research Methods
Rabia Javed, Noor Ul Ain, Ayesha Gul, Muhammad Arslan Ahmad, Weihong Guo, Qiang Ao, Shen Tian
Summary: Titanium dioxide nanoparticles (TiO2 NPs) are widely used metallic oxide nanoparticles. Doping can improve the properties of the nanoparticles and have significant effects in the field of biotechnology. The exponential growth of research on TiO2 NPs is due to their chemical stability, low toxicity, and multifaceted properties. They are extensively used in agriculture, environmental remediation, and nanomedicine.
IET NANOBIOTECHNOLOGY
(2022)
Review
Chemistry, Medicinal
Hengtong Zhang, Xixi Wu, Liang Quan, Qiang Ao
Summary: Oceans have great potential in developing high-value bioactive substances and biomaterials. Marine biomaterials are characterized by excellent biological activity and unique chemical structure, making them suitable for applications in tissue engineering and regenerative medicine.
Review
Polymer Science
Liang Quan, Yuan Xin, Xixi Wu, Qiang Ao
Summary: Self-healing hydrogels have the ability to automatically repair damage and avoid premature failure caused by mechanical damage. Researchers can design hydrogels to meet the needs of different tissues.
Review
Clinical Neurology
Rabia Javed, Qiang Ao
Summary: Nanobiotechnology offers potential for peripheral neural regeneration, providing an alternative treatment for peripheral nerve injuries. Autologous nerve grafts, the current standard treatment, have limitations and complications, making the development of alternative approaches necessary. Nanoparticles have therapeutic potential and understanding the fundamental mechanisms of peripheral nerve reconstruction is crucial for clinical translation.
JOURNAL OF NEURORESTORATOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Shabnam Anjum, Farheen Rahman, Prashant Pandey, Dilip Kumar Arya, Mahmood Alam, Paruvathanahalli Siddalingam Rajinikanth, Qiang Ao
Summary: This review examines the application of electrospun nanofibrous scaffolds in bone tissue engineering, demonstrating their potential for bone tissue regeneration. Nanofibrous scaffolds provide a natural environment for cell adhesion and function, and drug/growth factor delivery is also highlighted as a significant application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Sania Naz, Ayesha Gul, Muhammad Zia, Rabia Javed
Summary: The versatile nature of copper oxide nanoparticles (CuO NPs) has made them an important nanomaterial in the field of nanomedicine. Various preparation methods, including physical, chemical, and biological approaches, are used for CuO NPs synthesis. The physicochemical and biological properties of CuO NPs are mainly influenced by the fabrication method, making the selection of an appropriate synthetic technique crucial for specific biomedical applications. However, concerns about the biocompatibility of CuO NPs arise when intentionally using them in biomedicine.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Li-Li Wen, Tian-Hao Yu, Yi-Zhan Ma, Xiao-Yan Mao, Tian-Rang Ao, Rabia Javed, Hirotomo Ten, Akira Matsuno, Qiang Ao
Summary: This study established a method to enhance nerve regeneration by sequentially regulating the biological functions of endogenous Schwann cells (SCs). Injecting miR-221 and miR-338 through nerve conduits effectively improved the repair of damaged nerves. The findings suggest that this approach has potential for the clinical treatment of peripheral nerve injury.
NEURAL REGENERATION RESEARCH
(2023)
Article
Engineering, Biomedical
Ruiqi Wang, Yuchen Hua, Hongfeng Wu, Jingyu Wang, You-cai Xiao, Xuening Chen, Qiang Ao, Qin Zeng, Xiangdong Zhu, Xingdong Zhang
Summary: This study investigated the role of hydroxyapatite nanoparticles (HANPs) in manipulating the phenotype and function of macrophages in the tumor microenvironment. The results showed that the combination of HANPs and MPLA promoted cytokine secretion and polarization of macrophages towards a tumoricidal M1 phenotype. HANPs also induced immunogenic cell death in 4T1 breast tumor cells and activated macrophages towards an M1 type. In a tumor-bearing mice model, HANPs and MPLA synergistically delayed tumor growth.
ACTA BIOMATERIALIA
(2023)
Review
Pharmacology & Pharmacy
Yuan Xin, Liang Quan, Hengtong Zhang, Qiang Ao
Summary: Nanosystems-based antifungal agents have been effective in addressing drug resistance, drug release, and toxicity issues. Polymers, including polysaccharides, proteins, and polyesters, have gained significant attention as versatile materials for antifungal drug delivery. It is crucial for researchers to carefully select appropriate polymers based on specific scenarios when designing antifungal agent delivery nanosystems.
Review
Biochemistry & Molecular Biology
Rabia Javed, Anila Sajjad, Sania Naz, Humna Sajjad, Qiang Ao
Summary: The use of capping agents to prevent over-growth and coagulation of nanoparticles is an emerging trend. This strategy improves the physicochemical and biological behavior of nanoparticles, enhancing their reactivity while minimizing toxicity. Capping agents play a vital role in stabilizing the interface and have potential applications in nanomedicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
