Review
Oncology
Wenwen Guo, Caiqin Zhang, Tianyun Qiao, Jumei Zhao, Changhong Shi
Summary: This article summarizes strategies for the development and use of HIS mice models in tumor immunotherapies, proposes a method of tumor identification and classification in HIS mice based on the tumor-infiltrating lymphocytes and PD-L1 expression, and suggests different combination treatment strategies to enhance the effect of immunotherapy.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Sandrine Luce, Sophie Guinoiseau, Alexis Gadault, Franck Letourneur, Patrick Nitschke, Marc Bras, Michel Vidaud, Pierre Charneau, Etienne Larger, Maikel L. Colli, Decio L. Eizirik, Francois Lemonnier, Christian Boitard
Summary: The YES-RIP-hB7.1 mouse is a novel humanized model for studying T1D, showing spontaneous development of the disease along with T-cell responses to specific epitopes. This model is valuable for characterizing T1D-related epitopes and identifying factors triggering autoimmune responses in human T1D.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Jian Gao, Jinlin Miao, Haoyang Sun, Xianghui Fu, Peiyan Zhang, Zhinan Chen, Ping Zhu
Summary: This study successfully established a humanized mouse model of immune-related adverse effects and discovered that immune checkpoint inhibitors may lead to arthritis and pneumonitis. Additionally, TNF-alpha(+) T cells were identified as potential therapeutic targets for intervention in immune-related arthritis and pneumonitis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Wen Luo, Hai Hoang, Yanling Liao, Jian Pan, Janet Ayello, Mitchell S. Cairo
Summary: A humanized mouse model for Ewing sarcoma has been developed, allowing for functional reconstitution of the human immune system and evaluation of immunotherapy. This model holds promise for translational research in bone malignancies.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Nina B. Horowitz, Imran Mohammad, Uriel Y. Moreno-Nieves, Ievgen Koliesnik, Quan Tran, John B. Sunwoo
Summary: Innate lymphoid cells (ILCs) play an important role in antitumor immunity, but their behavior in the context of cancer is not fully understood. Humanized mouse models serve as excellent tools for studying these pathways and interactions between ILCs and tumors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Xin Chen, Xiaoshan Yang, Pingyun Yuan, Ronghua Jin, Lili Bao, Xinyu Qiu, Siying Liu, Tao Liu, John Justin Gooding, WanJun Chen, Guozhen Liu, Yongkang Bai, Shiyu Liu, Yan Jin
Summary: A novel therapeutic approach utilizing immune-homeostatic microparticles (IHMs) was developed to induce apoptosis in activated T cells, reestablish regulatory T cells, and treat multiple mouse models of autoimmunity. This strategy shows promise for efficiently treating autoimmune diseases by promoting immune tolerance.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Silvia Guil-Luna, Christine Sedlik, Eliane Piaggio
Summary: Immunotherapy plays a crucial role in cancer treatment, but not all patients respond. Research on humanized animal models is essential for overcoming research limitations and facilitating evaluation and further development of cancer immunotherapies.
ANNUAL REVIEW OF CANCER BIOLOGY, VOL 5, 2021
(2021)
Article
Oncology
Emre Kucukkose, Balthasar A. Heesters, Julien Villaudy, Andre Verheem, Madalina Cercel, Susan van Hal, Sylvia F. Boj, Inne H. M. Borel Rinkes, Cornelis J. A. Punt, Jeanine M. L. Roodhart, Jamila Laoukili, Miriam Koopman, Hergen Spits, Onno Kranenburg
Summary: A novel model for spontaneous multiorgan metastasis in MSI-H CRC tumors was created by transplanting PDO into humanized mice. Anti-PD-1 and anti-CTLA-4 ICB treatment were effective in reducing primary tumor mass and clearing liver metastases, but had no effect on peritoneal metastases. The efficacy of therapy correlated with the presence of TLS containing B cells and T cells, as well as an interferon-gamma signature and expression of the B cell chemoattractant CXCL13. B cell depletion prevented liver metastasis clearance by anti-CTLA-4 treatment. Ascites from peritoneal metastasis patients and the mouse model contained higher levels of immunosuppressive cytokines compared to serum samples.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Immunology
Alba Matas-Cespedes, Jean-Martin Lapointe, Matthew J. Elder, Gareth J. Browne, Simon J. Dovedi, Lolke de Haan, Shaun Maguire, Richard Stebbings
Summary: Humanized mice serve as valuable models to assess the impact of immunotherapeutics on the human immune system. Using NSG mice engrafted with cells predisposed to autoimmunity, the study evaluates the efficacy of monoclonal and BiSpecific antibodies targeting PD-1 and CTLA-4 in breaking immune tolerance. The results demonstrate that combination therapy or quadrivalent BiSpecific antibodies have enhanced potency, leading to accelerated development of GvHD.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Jing Wen, Lan Wang, Jie Ren, Emiko Kranz, Shilin Chen, Di Wu, Toshio Kanazawa, Irvin Chen, Yunfeng Lu, Masakazu Kamata
Summary: A humanized NSG(-Hc1) mouse model was developed with a corrected Hc gene mutation to support complement-dependent cytotoxicity, demonstrating its effectiveness in evaluating the efficacy of anticancer drugs. Nanoencapsulated rituximab showed enhanced antilymphoma activity in this humanized mouse model.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Review
Immunology
Jiaxuan Chen, Shuzhen Liao, Zengzhi Xiao, Quanren Pan, Xi Wang, Kangyuan Shen, Shuting Wang, Lawei Yang, Fengbiao Guo, Hua-feng Liu, Qingjun Pan
Summary: Animal models are essential for studying human diseases, but they do not fully replicate the complex internal environment of humans. Immunodeficient mice, lacking certain genes and cell populations, allow the establishment of humanized mouse models that simulate the human immune system. These models are widely used in disease research but face challenges that need to be addressed for further improvement.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Rheumatology
Sarah Julien, Bas van der Woning, Leentje De Ceuninck, Emma Briand, Thara Jaworski, Gaelle Roussel, Rachid Zoubairi, Yves Allenbach, Olivier Benveniste, Laurent Drouot, Olivier Boyer
Summary: In this study, the therapeutic effects of efgartigimod in immune-mediated necrotizing myopathies were evaluated using a humanized murine model. The results showed that efgartigimod rapidly reduced total IgG levels and prevented myofibre necrosis, allowing muscle fibre regeneration.
Article
Biology
A. K. Patel, Ankur Dhanik, Wei Keat Lim, Christina Adler, Min Ni, Yi Wei, Maggie Zhong, Cindy Nguyen, Jun Zhong, Yi-Fen Lu, Gavin Thurston, Lynn Macdonald, Andrew Murphy, Cagan Gurer, Davor Frleta
Summary: Immunodeficient mice reconstituted with a human immune system (HIS mice) are a valuable tool for studying human immune responses to tumors. However, these mice often exhibit sub-optimal immune responses and lack antigen-specific B or T cell memory. In this study, we observed spontaneous regression of human B cell lymphoma Raji in HIS mice. Tumor regression was dependent on CD4+ and CD8+ T cells and resulted in T cell memory. We also found clonal expansion of specific T cell receptors (TCRs) and Raji-specific T cell reactivity in regressing tumors.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biology
Wen Lin, Varan Singh, Raynel Springer, Gabrielle Choonoo, Namita Gupta, Aditi Patel, Davor Frleta, Jun Zhong, Tomasz Owczarek, Corinne Decker, Lynn Macdonald, Andrew Murphy, Gavin Thurston, Markus Mohrs, Ella Ioffe, Yi-Fen Lu
Summary: A critical role of human CD4 cytotoxic T lymphocytes in mediating tumor clearance independent of CD8 T cells is reported in humanized immune system mice implanted with HT-29 colorectal tumors.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jonathan Schupp, Arne Christians, Niklas Zimmer, Lukas Gleue, Helmut Jonuleit, Mark Helm, Andrea Tuettenberg
Summary: The presence and interaction of immune cells in the tumor microenvironment have a significant impact on disease progression and treatment response. Spatial analyses are crucial in the complex interplay between neoplastic and immune cells, aiming to develop new therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pathology
Nikunj Tandel, Anish Z. Joseph, Aishwarya Joshi, Priya Shrama, Ravi P. N. Mishra, Rajeev K. Tyagi, Prakash S. Bisen
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2020)
Article
Engineering, Manufacturing
Monic Sun, Rajeev K. Tyagi
PRODUCTION AND OPERATIONS MANAGEMENT
(2020)
Article
Chemistry, Multidisciplinary
Ramesh Chaudhari, Nikunj Tandel, Kiran Sahu, Sushmita Negi, Hilal Bashir, Arzu Rupareliya, Ravi Pn Mishra, Sarat K. Dalai, Rajeev K. Tyagi
Summary: Elastic liposomes are efficient nanocarriers that enhance the immune response in transdermal immunization. Subcutaneous delivery mediated by elastic liposomes can generate better immune responses compared to intramuscular injection.
Article
Immunology
Rajeev K. Tyagi
Summary: Extensive research on mouse-human chimeras has contributed to our understanding of infectious diseases, especially in the case of the human-malaria parasite Plasmodium falciparum. The complex life cycle and genome of P. falciparum, along with drug resistance issues, pose challenges for parasitology research. Utilizing human cell/tissue reconstituted immunodeficient mice is a promising approach for studying these aspects and addressing the lack of effective vaccine and drug resistance concerns.
Review
Cell Biology
Sushmita Negi, Sheetal Saini, Nikunj Tandel, Kiran Sahu, Ravi P. N. Mishra, Rajeev K. Tyagi
Summary: Crohn's disease and ulcerative colitis are two major forms of inflammatory bowel disease in humans, caused by dysregulated immune response against constituents of gut flora in genetically predisposed individuals. Defective immune responses against regulatory mechanisms amplify and maintain IBD-induced mucosal inflammation.
Article
Business
Sreya Kolay, Rajeev K. Tyagi
Summary: We study a seller's optimal bundling strategy for selling two events offered over time, taking into account the temporal and perishability features of events, uncertainty in consumer valuation, and the impact of market features on the seller's optimal strategy choices.
Article
Economics
Sreya Kolay, Rajeev K. Tyagi
Summary: This study examines the effects of a rival's cost increase on product quality in a vertically differentiated market, providing conditions to explain whether a company gains or loses when its competitor's costs increase, and how this gain or loss depends on consumer choices, consumer taste for quality, quality production costs, and competing firms' production efficiencies.
REVIEW OF INDUSTRIAL ORGANIZATION
(2022)
Article
Microbiology
Naisargee Patel, Aarushi Zinzuvadia, Mitali Prajapati, Rajeev K. Tyagi, Sarat Dalai
Summary: Swertiamarin has anti-inflammatory and immunomodulatory effects on malaria parasite in experimental mice, delaying parasite development. Regular application of swertiamarin modulates IFN-gamma and IL-10 levels. However, the short half-life of swertiamarin limits its long-lasting therapeutic effect, requiring higher and more frequent dosing schedules.
FUTURE MICROBIOLOGY
(2022)
Review
Oncology
Megan L. Tigue, Matthew A. Loberg, Jeremy A. Goettel, William A. Weiss, Ethan Lee, Vivian L. Weiss
Summary: Tumor-associated macrophages (TAM) have a significant impact on tumor growth and immune responses, with TAM infiltration being linked to poor prognosis in various cancers. TAMs can be categorized as pro-inflammatory, M1-like, or anti-inflammatory, M2-like. Recent evidence suggests that Wnt signaling plays a crucial role in promoting an immunosuppressive, M2-like phenotype in TAMs, facilitating cancer cell proliferation, invasion, and metastasis. Targeting TAM polarization and their protumorigenic functions through Wnt signaling inhibitors could potentially be an effective treatment strategy in cancers with a high presence of macrophages in the microenvironment.
Review
Immunology
Nikunj Tandel, Sushmita Negi, Sarat K. Dalai, Rajeev K. Tyagi
Summary: Human natural killer cells (hNKs) and other ILCs interact with B cells to induce pathogen-specific immune responses and play a crucial role in clearing infectious pathogens in diseases.
INTERNATIONAL REVIEWS OF IMMUNOLOGY
(2023)
Review
Virology
Reetesh Kumar, Yogesh Srivastava, Pandiyan Muthuramalingam, Sunil Kumar Singh, Geetika Verma, Savitri Tiwari, Nikunj Tandel, Samir Kumar Beura, Abhishek Ramachandra Panigrahi, Somnath Maji, Prakriti Sharma, Pankaj Kumar Rai, Dinesh Kumar Prajapati, Hyunsuk Shin, Rajeev K. Tyagi
Summary: Genetic variants of SARS-CoV-2, including mutations in the spike glycoprotein gene, are emerging worldwide and could have significant impacts on transmission, infectivity, virulence, and evasion of the host immune system. These mutations may affect vaccine effectiveness, antigenic diversity, antibody interactions, protein stability, RBD flexibility, and accessibility to the human cell receptor ACE2. This study provides valuable insights for designing next-generation vaccines and biotherapeutics against COVID-19.
Letter
Toxicology
Gregory J. Garvey, Janet K. Anderson, Philip P. Goodrum, Kirby H. Tyndall, L. Anthony Cox, Mahin Khatami, Jorge Morales-Montor, Rita S. Schoeny, Jennifer G. Seed, Rajeev K. Tyagi, Christopher R. Kirman, Sean M. Hays
CRITICAL REVIEWS IN TOXICOLOGY
(2023)
Article
Engineering, Multidisciplinary
Sumit Sharma, Gopal Nandan, R. K. Tyagi
Summary: The demand for novel and self-healing material is a significant challenge in industries nowadays. Many engineering applications require materials with tailored properties, including self-healing characteristics. This study developed a metal matrix composite of aluminum A356 alloy using a semi-solid metal processing technique and utilizing the properties of Nitinol alloy wire. The investigation was validated through light-microscope images and field emission scanning electron microscopy images were taken for morphological examination. The results showed that the reinforcement was randomly dispersed in the matrix phase and the elemental confirmation was conducted through Energy Dispersive X-Ray Analysis (EDX) spectrum. The crack analysis before and after healing revealed that the interlocks of Nitinol wire with the alpha Al phase of the base alloy can close the crack and recover the deformed surface by 17.35%.
ENGINEERING RESEARCH EXPRESS
(2023)
Review
Toxicology
Gregory J. Garvey, Janet K. Anderson, Philip E. Goodrum, Kirby H. Tyndall, L. Anthony Cox, Mahin Khatami, Jorge Morales-Montor, Rita S. Schoeny, Jennifer G. Seed, Rajeev K. Tyagi, Christopher R. Kirman, Sean M. Hays
Summary: An independent international expert panel found moderate evidence that PFOS and PFOA are immunotoxic based on animal data. However, limitations in data make it difficult to establish species concordance and human relevance. The panel recommends further testing and research to overcome these limitations and better understand the immunotoxicity of PFAS.
CRITICAL REVIEWS IN TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Paola Orlandi, Marta Banchi, Francesca Vaglini, Marco Carli, Stefano Aringhieri, Arianna Bandini, Carla Pardini, Cristina Viaggi, Michele Lai, Greta Ali, Alessandra Ottani, Eleonora Vandini, Patrizia Guidi, Margherita Bernardeschi, Veronica La Rocca, Giulio Francia, Gabriella Fontanini, Mauro Pistello, Giada Frenzilli, Daniela Giuliani, Marco Scarselli, Guido Bocci
Summary: This study investigates the role of MC4R in melanoma and the use of the selective antagonist ML in combination with vemurafenib. The results show that ML can inhibit melanoma cell proliferation and induce apoptosis through the inhibition of ERK1/2 phosphorylation and reduction of BCL-XL expression. The combination of vemurafenib and ML exhibits a synergistic effect in vitro and inhibits tumor growth in vivo without causing adverse effects.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Conor J. Bloxham, Katina D. Hulme, Fabrizio Fierro, Christian Fercher, Cassandra L. Pegg, Shannon L. O'Brien, Simon R. Foster, Kirsty R. Short, Sebastian G. B. Furness, Melissa E. Reichelt, Masha Y. Niv, Walter G. Thomas
Summary: Bitter taste receptors (T2Rs) are a type of G protein-coupled receptors that allow humans to detect aversive and toxic substances. This study characterized the functional properties of previously identified T2Rs in human cardiac tissues and their naturally occurring polymorphisms. The results showed differences in signaling among different T2R variants, and revealed a potential association between the T2R50 Tyr203 variant and cardiovascular disease.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Lu Chen, Huanying Shi, Wenxin Zhang, Yongjun Zhu, Haifei Chen, Zimei Wu, Huijie Qi, Jiafeng Liu, Mingkang Zhong, Xiaojin Shi, Tianxiao Wang, Qunyi Li
Summary: This study demonstrates that Carfilzomib exhibits potent anti-tumor activity against esophageal squamous cell carcinoma (ESCC) by triggering mitochondrial apoptosis and reprogramming cellular metabolism. It has been identified that activating transcription factor 3 (ATF3) plays a crucial role as a cellular target in ESCC cells treated with Carfilzomib. Overexpression of ATF3 effectively counteracts the effects of Carfilzomib on ESCC cell proliferation, apoptosis, and metabolic reprogramming. Furthermore, ATF3 mediates the anti-tumor activity of Carfilzomib, suggesting its potential as a therapeutic agent for ESCC.
BIOCHEMICAL PHARMACOLOGY
(2024)
Review
Pharmacology & Pharmacy
Xing Zhang, Xiang Li, Ran Xia, Hong-Sheng Zhang
Summary: This review summarizes recent progress on the mechanisms of ferroptosis resistance in cancer and highlights the role of redox status and metabolism. Combination therapy for ferroptosis has great potential in treating resistant malignant tumors.
BIOCHEMICAL PHARMACOLOGY
(2024)
