Journal
BIOFOULING
Volume 32, Issue 2, Pages 123-134Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2015.1125472
Keywords
Biofilms; Candida; infection models; Streptococcus; Porphyromonas; peri-implantitis
Funding
- Fundo de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil [2012/07436-6]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil [007355/2013-00]
Ask authors/readers for more resources
Whilst Candida albicans occurs in peri-implant biofilms, its role in peri-implantitis remains unclear. This study therefore examined the virulence of C. albicans in mixed-species biofilms on titanium surfaces. Biofilms of C. albicans (Ca), C. albicans with streptococci (Streptococcus sanguinis, S. mutans) (Ca-Ss-Sm) and those incorporating Porphyromonas gingivalis (Ca-Pg and Ca-Ss-Sm-Pg) were developed. Expression of C. albicans genes associated with adhesion (ALS1, ALS3, HWP1) and hydrolytic enzymes (SAP2, SAP4, SAP6, PLD1) was measured and hyphal production by C. albicans quantified. Compared with Ca biofilms, significant (p<0.05) up-regulation of ALS3, HWP1, SAP2 and SAP6, and hyphal production occurred in biofilms containing streptococci (Ca-Ss-Sm). In Ca-Pg biofilms, down-regulation of HWP1 and SAP4 expression, with reduced hyphal production occurred. Ca-Ss-Sm-Pg biofilms had increased hyphal proportions and up-regulation of ALS3, SAP2 and SAP6. In conclusion, C. albicans expressed virulence factors in biofilms that could contribute to peri-implantitis, but this was dependent on associated bacterial species.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available