Article
Microbiology
Chiau-Jing Jung, Chih-Chieh Hsu, Jeng-Wei Chen, Hung-Wei Cheng, Chang-Tsu Yuan, Yu-Min Kuo, Ron-Bin Hsu, Jean-San Chia
Summary: The LiaR-regulated PCP protein in Streptococcus mutans plays a crucial role in bacterial biofilm formation and platelet adhesion. It is well-preserved among pathogenic streptococci and controls biofilm formation in vivo, suggesting its importance in colonization for S. mutans and possibly other pathogenic streptococci.
Article
Biochemistry & Molecular Biology
Marcin Magacz, Sergio Alatorre-Santamaria, Karolina Kedziora, Kacper Klasa, Pawel Mamica, Wiktoria Pepasinska, Magdalena Lebiecka, Dorota Koscielniak, Elzbieta Pamula, Wirginia Krzysciak
Summary: The LPO system has potential in preventing dental caries by exhibiting antimicrobial properties. Modifying the enzymatic system using different substrates showed varying effects on biofilm growth and lactate synthesis. The LPO-iodide system had the strongest inhibitory effect on biofilm growth, while the LPO-selenocyanate system showed a moderate inhibitory effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
So-Young Ham, Han-Shin Kim, Eunji Cha, Taehyeung Lim, Youngjoo Byun, Hee-Deung Park
Summary: Raffinose, a natural oligosaccharide, strongly inhibits biofilm formation and GTF-related gene expression in Streptococcus mutans, making it a potential antibiofilm agent for oral supplies and dental materials to prevent dental caries.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Multidisciplinary
Ji-Eun Lee, Hee Yeon Cho, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik
Summary: This study investigated the inhibitory effects of ovomucoid, a protein found in egg white, on the biofilm formation of Streptococcus mutans, the primary pathogen associated with tooth decay. The results showed that ovomucoid inhibited biofilm formation, reduced bacterial adhesion and aggregation, and lowered the metabolic activity of bacteria in the biofilm. In addition, ovomucoid decreased the synthesis of water-insoluble EPS by S. mutans. Therefore, ovomucoid has the potential to be used as an anti-biofilm agent for the treatment of dental caries.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Immunology
Shota Mayumi, Masae Kuboniwa, Akito Sakanaka, Ei Hashino, Asuka Ishikawa, Yura Ijima, Atsuo Amano
Summary: Recent studies have shown phenotypic and metabolic heterogeneity in related species including Streptococcus oralis, Streptococcus mutans, and Streptococcus gordonii. D-tagatose was found to be abundant in the saliva of individuals with good oral hygiene, and it selectively inhibited the growth of oral pathogens S. mutans and S. gordonii while minimally affecting the oral commensal S. oralis. This suggests the potential use of D-tagatose as an oral prebiotic for manipulating the metabolism of targeted oral streptococci.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Rodrigo A. Giacaman, Rodrigo Umana, Maria Jose Nunez, Natalia Diaz-Garrido, Constanza Echeverria, Natalia Garcia-Manriquez, Alex Mira, Constanza E. Fernandez, Karla Gambetta-Tessini, Carla P. Lozano
Summary: This study aimed to investigate the impact of saliva dilutions on an in vitro caries model using Streptococcus mutans biofilms. The results showed that even small quantities of saliva could significantly reduce demineralization in enamel and dentin, and the protective effect of saliva was dose-dependent.
Article
Microbiology
Muna Aqawi, Ronit Vogt Sionov, Ruth Gallily, Michael Friedman, Doron Steinberg
Summary: The study demonstrated that Cannabigerol (CBG) can effectively prevent the formation of Streptococcus mutans biofilms, reduce biofilm thickness and metabolic activity, and alter the surface roughness of the biofilms. CBG also decreased the expression of genes related to S. mutans biofilm formation, indicating its potential as a preventive treatment for dental caries.
Article
Food Science & Technology
Guofang Zhang, Ying Tan, Tianshu Yu, Sitong Wang, Libo Liu, Chun Li
Summary: The combination of reuterin and catechin shows synergistic antimicrobial activity against Streptococcus mutans by inhibiting growth, biofilm formation, water-insoluble glucan production, and virulence genes expression. This novel combination demonstrates potential as a natural antibacterial agent for the prevention of oral diseases.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
B. Holguin-Loya, U. Soto-Barreras, R. Martinez-Martinez, G. Martinez-Mata, L. Sanin, M. Nevarez-Rascon, R. Dominguez-Perez
Summary: This study investigated the association between count of S. mutans in supragingival biofilm and fluoride exposure in scholar children, finding that fluoride levels in urine and saliva were negatively associated with the count of S. mutans, while plaque index, D3-6MFS and time of last visit to the dentist showed a positive association, indicating the potential key role of fluoride in reducing the risk of dental caries.
JOURNAL OF DENTAL SCIENCES
(2022)
Review
Immunology
Xiuqing Wang, Jingling Li, Shujun Zhang, Wen Zhou, Linglin Zhang, Xiaojing Huang
Summary: Dental biofilms are highly organized bacterial communities protected by an extracellular matrix. These biofilms can become acidic under hostile conditions, leading to an imbalance in the microbial population and ultimately causing dental caries. Recent research has focused on designing pH-activated strategies to control dental caries, such as pH-responsive antimicrobial agents and drug delivery systems. These strategies selectively target acidic biofilms without disrupting the neutral microenvironment and biodiversity in the mouth.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Xiuqin Chen, Akanksha Tyagi, Selvakumar Vijayalakshmi, Ramachandran Chelliah, Umair Shabbir, Deog-Hwan Oh
Summary: This study found that the combination of slightly acidic electrolyzed water (SAEW) and sodium benzoate (NaB) can effectively control Streptococcus mutans biofilm, reducing its adhesive strength and decreasing the height of the biofilm. This could be a promising new approach for treating dental caries.
MICROBIAL PATHOGENESIS
(2022)
Article
Biotechnology & Applied Microbiology
Luting Weng, Lang Wu, Rongjuan Guo, Jiajia Ye, Wen Liang, Wei Wu, Liang Chen, Deqin Yang
Summary: This study successfully prepared nanoparticles, called LA/TCS@PLGA-NPs, with favorable properties using cell membrane coating technology and Lactobacillus strains. The nanoparticles were able to adhere to Streptococcus mutans and interfere with its biofilm formation, leading to significant inhibition of the activity, biomass, and virulence gene expression of the biofilms. In vivo experiments also showed that LA/TCS@PLGA-NPs had a long-lasting inhibitory effect on the progression of dental caries. These nanoparticles could be considered as a new class of effective drug delivery systems for caries prevention.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yuting Tian, Yue Zhang, Mengjiao Zhang, Xianchun Chen, Lei Lei, Tao Hu
Summary: The purpose of this study was to construct a suitable vector to carry and protect antisense vicR RNA (ASvicR), and investigate its effects on the cariogenicity of S. mutans. Aminated dendritic mesoporous silica nanoparticles (DMSNs-NH2) were found to be a suitable carrier for ASvicR, reducing EPS synthesis and biofilm formation of S. mutans.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Zhimin Hu, Ying Tang, Bulin Jiang, Yue Xu, Siying Liu, Cui Huang
Summary: This study aimed to avoid antibiotic resistance by using curcumin, a natural plant extract with photodynamic effects, to exhibit antibacterial activity against Streptococcus mutans (S. mutans) biofilms. To overcome the limitations of curcumin, researchers constructed a curcumin-loaded liposome with high drug loading efficiency, stability, controlled release, and biocompatibility. The results showed that the liposome effectively adhered to and inhibited the growth of S. mutans biofilms. Therefore, curcumin-loaded liposomes have the potential for clinical application in the treatment of dental caries.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Li Yan, Sha Zhang, Xiaoying Zhou, Shuge Tian
Summary: This study investigated the antibacterial ability and inhibitory mechanisms of orientin-2''-O-beta-L-galactoside, orientin, and vitexin against Streptococcus mutans. The results showed that these natural flavonoids had inhibitory effects on S. mutans and reduced EPS formation while stimulating the release of LDH. They also inhibited biofilm formation and down-regulated the transcription levels of various genes in S. mutans.
Article
Pharmacology & Pharmacy
Guilherme M. Gelfuso, Ricardo Ferreira-Nunes, Luciana F. Dalmolin, Ana C. dos S. Re, Giselly A. dos Santos, Fernando A. P. de Sa, Marcilio Cunha-Filho, Antonio Alonso, Sebastiao A. Mendanha Neto, Jorge L. Anjos, Carolina P. Aires, Renata F. Lopez, Tais Gratieri
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Instruments & Instrumentation
Ana Carolina S. Re, Jayanaraian F. Martins, Marcilio Cunha-Filho, Guilherme M. Gelfuso, Carolina P. Aires, Tais Gratieri
Summary: Candidiasis is a common opportunistic infection caused by fungi of the Candida genus, mainly affecting mucocutaneous tissues. Recurrent superficial candidiasis remains a challenge despite numerous available treatments, with biofilms playing a key role in antifungal resistance. Current research is focused on technological advancements in formulations to address the challenges, such as enhancing drug delivery system contact and permeation.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
J. G. Neves, P. D. Marcato, F. W. G. de Paula e Silva, C. P. T. Mantovani, H. S. Prado, C. P. Aires, T. N. C. Massaro, M. C. Borsato
Summary: The NPL-chitosan nanoparticles showed antibacterial activity against S. mutans, with MIC of 0.4% and MBC of 0.67%, and presented stable characteristics for at least 75 days. There was no statistical difference in Microtensile Strength among the experimental groups, but the group with 2% of NPL-chitosan exhibited higher MMP inactivation activity compared to others.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Pedro Castania Amadio Domingues, Viviane de Cassia Oliveira, Felipe Lazarini Bim, Carolina Patricia Aires, Andre Pereira dos Santos, Denise Tornavoi de Castro, Claudia Helena Silva-Lovato, Denise de Andrade, Evandro Watanabe
Summary: This study examined the impact of different glucose concentrations on biofilm formation of Candida albicans and Candida glabrata strains isolated from diabetic and non-diabetic individuals. The results showed that the strain type had an influence on biofilm formation, but glucose supplementation did not.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Physics, Multidisciplinary
C. P. Aires, A. C. Polizello, A. Caliri, S. Mascarenhas
Summary: Communication among living structures is an important parameter in biology, and this study provides evidence for bacterial communication using a dedicated experiment on Bacillus subtilis colonies.
BRAZILIAN JOURNAL OF PHYSICS
(2022)
Review
Biotechnology & Applied Microbiology
Maria Gerusa Brito Aragao, Carolina Patricia Aires, Silmara Aparecida Milori Corona
Summary: EGCG can suppress the acid production of Streptococcus mutans by inhibiting enzyme activity and affect its virulence factors, but its effects on biofilms are still unclear. There is high variability in the research, and further studies are needed to confirm the effectiveness of EGCG against S. mutans biofilms.
Article
Biochemistry & Molecular Biology
Sofia Gerotto Viola, Luciana Facco Dalmolin, Janette Betzabeth Villarruel Munoz, Yugo Araujo Martins, Ana Carolina dos Santos Re, Carolina Patricia Aires, Renata Fonseca Vianna Lopez
Summary: This study investigated the antimicrobial effect of anodic iontophoresis on Staphylococcus epidermidis and Escherichia coli. The results showed that anodic iontophoresis inhibited the growth of Staphylococcus epidermidis and caused death in Escherichia coli. It can be a powerful ally in controlling the proliferation of Escherichia coli in wounds.
BIOELECTROCHEMISTRY
(2023)
Article
Microbiology
Jessica Silva Peixoto Bem, Nayanna Gomes Silva Lacerda, Ana Cristina Morseli Polizello, Hamilton Cabral, Nathalia Gonsales da Rosa-Garzon, Carolina Patricia Aires
Summary: This study evaluated the disruptive effect of fungal mutanase on cariogenic biofilm. Mature Streptococcus mutans biofilms were exposed to fungal enzymes for short-term treatment. The active enzymes significantly reduced insoluble polysaccharide content in the biofilm compared to the negative control group, without affecting bacterial viability and metabolism. These findings suggest that fungal mutanase could be used in oral care products.
CURRENT MICROBIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Vanessa Tonetto Marques Galves, Ellen Emanuela Cintra Monteiro, Fernanda Fortes Cabral, Carolina Patricia Aires, Ana Cristina Morseli Polizello, Maria Teresa Botti Rodrigues Santos, Lara Maria Alencar Ramos Innocentini, Graziela Vieira Cavalcanti, Fabiola Reis de Oliveira, Luiz Carlos Conti de Freitas, Eduardo Melani Rocha, Maria da Conceicao Pereira Saraiva, Ana Carolina Fragoso Motta
Summary: This study compared the clinical profiles and oral health status of patients with Sjogren's syndrome (SS), patients with dry symptoms without conclusive diagnosis of SS (non-SS), and a control group. The results showed that both SS and non-SS patients had poorer oral health, particularly dental and periodontal problems, which negatively impacted their quality of life.
ORAL SCIENCE INTERNATIONAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Fernanda Vicioni-Marques, Francisco Wanderley Garcia de Paula-Silva, Milena Rodrigues Carvalho, Alexandra Mussolino de Queiroz, Osvaldo de Freitas, Maira Peres Ferreira Duarte, David John Manton, Fabricio Kitazono de Carvalho
Summary: Molar incisor hypomineralization (MIH) is often accompanied by dental hypersensitivity and difficulty in achieving effective analgesia. This study evaluated the effectiveness of preemptive analgesia in children with severe MIH, post-eruptive enamel breakdown, and hypersensitivity. The results showed that preemptive analgesia improved the effectiveness of infiltrative anesthesia and patient comfort during restorative procedures.
JOURNAL OF APPLIED ORAL SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Larisse Eduardo Adami, Osvaldo de Freitas, Fellipe Augusto Tocchini de Figueiredo, Maira Peres Ferreira, Ana Paula Macedo, Rene Oliveira do Couto, Vinicius Pedrazzi
Summary: This study demonstrates the clinical efficacy of mucoadhesive patches designed for needle-free pre-operative local anesthesia in dentistry. The patches were shown to initiate anesthesia within a short time frame, reaching maximum effect within 15 to 25 minutes, and lasting at least 50 minutes without any reported side effects.
BRAZILIAN ORAL RESEARCH
(2021)
Article
Materials Science, Biomaterials
Michael Oluwole Osungunna, Andre Pitondo-Silva, Leticia Bueno Silva, Ana Carolina S. Re, Priscyla D. Marcato, Tais Nader Chrysostomo Massaro, Ana Cristina M. Polizello, Carolina Patricia Aires
Summary: The study prepared nanostructured lipid carriers coated with chitosan to control the growth of uropathogenic E. coli biofilms. It was found that the NLC-chitosan affected bacterial viability in biofilms of different ages, demonstrating its effectiveness in controlling biofilm growth.
Article
Chemistry, Medicinal
Frederico Severino Martins, Sherwin K. B. Sy, Edemilson Cardoso da Conceicao, Maria Jose Vieira Fonseca, Osvaldo de Freitas
Summary: The study optimized a topical gel formulation for the treatment of vitiligo, demonstrating good pharmacokinetic and pharmacodynamic properties in a mouse model.
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2021)
Article
Multidisciplinary Sciences
Giselly Almeida dos Santos, Ricardo Ferreira-Nunes, Luciana Facco Dalmolin, Ana Carolina dos Santos Re, Jorge Luiz Vieira Anjos, Sebastiao Antonio Mendanha, Carolina Patricia Aires, Renata F. V. Lopez, Marcilio Cunha-Filho, Guilherme M. Gelfuso, Tais Gratieri
SCIENTIFIC REPORTS
(2020)
