Journal
BIOESSAYS
Volume 38, Issue 8, Pages 744-750Publisher
WILEY-BLACKWELL
DOI: 10.1002/bies.201600038
Keywords
fluorescently-tagged mRNA; high resolution fluorescence microscopy; mRNA export; mRNP maturation; mRNP quality control; nuclear pore complex; single particle tracking
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada
- Canadian Institutes of Health Research
- Ontario Graduate Scholarship
Ask authors/readers for more resources
Six years ago, the Singer lab published a landmark paper which described how individual mRNA particles cross the nuclear pore complex in mammalian tissue culture cells. This involved the simultaneous imaging of mRNAs, each labeled by a large number of tethered fluorescent proteins and fluorescently tagged nuclear pore components. Now two groups have applied this technique to the budding yeast Saccharomyces cerevisiae. Their results indicate that in the course of nuclear export, mRNAs likely engage complexes that are present on either side of the pore and that these interactions are modulated by proteins present in the messenger ribonucleoprotein (mRNP) complex. These findings lend support to the notion that just before and/or after the completion of nuclear export, mRNPs undergo one or more maturation steps that prepare the packaged mRNAs for translation. These results represent new and exciting insights into the mechanism of mRNA nuclear export.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available