Article
Food Science & Technology
Shifeng Pang, Xiangmin Piao, Xiaohao Zhang, Xiaolin Chen, Hao Zhang, Yinping Jin, Zheng Li, Yingping Wang
Summary: In this study, a metabolomic method based on the UPLC-Orbitrap fusion platform was established to determine and analyze the metabolites of American ginseng from different geographical origins. A total of 382 metabolites were detected, and 20 metabolites were identified as potential markers that could differentiate the producing areas with high accuracy. This study provides new insights into the metabolite composition of American ginseng and can help in origin discrimination and quality control.
FOOD SCIENCE & NUTRITION
(2023)
Article
Multidisciplinary Sciences
Lele Zhang, Shaofei Song, Biying Chen, Rongrong Li, Liming Wang, Chenxi Wang, Lifeng Han, Zhifei Fu, Zhonglian Zhang, Qilong Wang, Heshui Yu
Summary: The aim of this study was to analyze the anti-inflammatory activity and chemical composition of different species of Lasianthus and explore their active compounds. The anti-inflammatory activity of seven species of Lasianthus was evaluated through cell experiments, and metabolomics and mass defect filter (MDF) algorithm were used to explore metabolites. Through correlation analysis and pharmacological validation, seven compounds were confirmed as anti-inflammatory compounds. Metabolomics integrated with activities evaluation might be a rapid and effective strategy to explore the active compounds from natural products.
Article
Agriculture, Multidisciplinary
Chunxia Zhang, Meiyu Liu, Xiaoyan Xu, Jia Wu, Xue Li, Hongda Wang, Xiumei Gao, Dean Guo, Xiaoxuan Tian, Wenzhi Yang
Summary: This research evaluated the coverage of data-dependent acquisition (DDA) in untargeted metabolite/component identification in the food sciences and pharmaceutical analysis. Using Panax ginseng flower buds as an example, DDA on a Q-Orbitrap mass spectrometer demonstrated higher coverage compared to other MS2 acquisition approaches. By predicting a large-scale molecular library and filtering based on mass defect, new component MS2 spectra were obtained and unknown ginsenosides were characterized. In conclusion, a molecular-prediction-oriented DDA approach can enable the discovery of potentially novel molecules for functional food and drug development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Meiyu Liu, Xiaoyan Xu, Xiaoyan Wang, Hongda Wang, Yueguang Mi, Xiumei Gao, Dean Guo, Wenzhi Yang
Summary: The study successfully constructed a PIL-based DDA strategy and applied it to the identification of ginsenosides. The results showed significant advantages of this strategy in identifying both known and unknown ginsenosides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Heyu Wang, Rensong Huang, Hui Li, Lili Jiao, Shuying Liu, Wei Wu
Summary: The study demonstrated the anti-diabetic and anti-oxidative effects of ginseng berry, showing that GB ginsenoside treatment significantly decreased blood glucose levels, increased serum SOD content, and reduced malondialdehyde content. Metabolic pathway analysis indicated that GB ginsenoside regulates pathways such as bile acid metabolism to treat T2DM.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Microbiology
Chen Zhang, Yanyan Xu, Mengmeng Gu, Zhenzhen Liu, Jingyuan Zhang, Qi Zeng, Daheng Zhu
Summary: In this study, an endophytic bacterium G9y was isolated and identified from Panax quinquefolius, which was capable of converting ginsenoside Rc into Rd. The optimized biotransformation conditions led to complete conversion of Rc to Rd within 25 h after inoculation. This discovery provides a new microbial resource for preparing ginsenoside Rd and explores a pathway for Rc utilization, showing great potential for application.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Xianmei Yin, Haoyu Hu, Xiaofeng Shen, Xiangyan Li, Jin Pei, Jiang Xu
Summary: Ginseng, known as the king of herbs, has been used as an important traditional medicine for thousands of years. Ginsenosides, bioactive compounds of ginseng, have posed challenges for research and development due to their complexity. In recent years, the emergence of new biological research tools, particularly omics and bioinformatic tools, has become a major trend in ginseng research.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2021)
Review
Plant Sciences
Paul H. Goodwin, Madison A. Best
Summary: Ginsenosides have been widely studied for their medicinal benefits and their role in ginseng plant biology. They play a crucial role in ginseng's defense against biotic stresses, providing antimicrobial, antifeedant, and allelopathic activities. Ginsenosides also have roles in ginseng development and abiotic stress tolerance.
Article
Biochemical Research Methods
Li Jia, Hongda Wang, Xiaoyan Xu, Huimin Wang, Xue Li, Ying Hu, Boxue Chen, Meiyu Liu, Xiumei Gao, Huifang Li, Dean Guo, Wenzhi Yang
Summary: Comprehensively elucidating herbal metabolites is crucial in natural products research for the discovery of new lead compounds. This study presents an offline multidimensional chromatography/high-resolution mass spectrometry approach which successfully identified a large number of ginsenosides from the flower buds of Panax plants, including potentially unknown compounds.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Zhen Du, Huanjun Wang, Xueling Li, Meiyue Dong, Bingqing Chi, Zhenhua Tian, Zhenguo Wang, Haiqiang Jiang
Summary: The purpose of this study was to develop an improved comprehensive data filtering strategy, implemented through Excel software, for screening potential 2-(2-phenylethyl)chromone (PEC) monomers and PEC dimers from agarwood. A total of 108 PEC monomers and 30 PEC dimers in agarwood were characterized. The results provide useful information for future utilization of agarwood, with the first in-depth analysis of MS/MS fragmentation behavior and identification of substituent positions of a large number of PEC monomers and PEC dimers.
Article
Chemistry, Analytical
Wenwen Huang, Zhonglian Zhang, Lu Niu, Xiaohan Hu, Tekleab Teka, Lifeng Han, Guixiang Pan, Qilong Wang
Summary: This study explored the vasorelaxation effects of different species of Uncaria on isolated rat mesenteric artery rings, identified potential vasodilative compounds through metabolite profiling and correlation analysis. The results suggest that metabolomics combined with correlation analysis is an effective strategy for rapidly discovering active compounds in traditional Chinese medicine.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Food Science & Technology
Prasad Kesanakurti, Subramanyam Ragupathy, Adam C. Faller, Dhivya Shanmughanandhan, Francesco Buongiorno, Isabella Della Noce, Zhengfei Lu, Yanjun Zhang, Steven G. Newmaster
Summary: Authentication of Panax ginseng and Panax quinquefolius herbal dietary supplements is crucial in mitigating adulteration and substitution issues in the international ginseng supply chain. Species-specific hydrolysis probe qPCR assays have been developed and validated, demonstrating high specificity and reliability for the identification of target species in raw materials.
Article
Agriculture, Multidisciplinary
Anli Wang, Xinyu Chen, Shanyun Wu, Wei Jia, Jingjing Jiao, Yu Zhang
Summary: This study evaluated the metabolic profiles in rat serum after exposure to acrylamide, revealing significant changes in metabolites in rats exposed to high doses of acrylamide, mainly affecting pathways such as the citrate cycle, amino acid biosynthesis, and pyruvate metabolism.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Meiyue Dong, Zhenhua Tian, Yanni Ma, Zhongyi Yang, Zhen Ma, Xiaoming Wang, Yunlun Li, Haiqiang Jiang
Summary: This study developed an integrated data filtering and identification strategy to characterize the GSLs in 25 commonly Brassicaceae tissues, successfully identifying 47 GSLs and establishing profiles for each tissue, which can be used to distinguish different Brassicaceae species.
Article
Pharmacology & Pharmacy
Rong Dong, Yougang Zhang, Shanjun Chen, Huan Wang, Kaiqing Hu, Huanxin Zhao, Qingping Tian, Kewu Zeng, Songsong Wang, Liwen Han
Summary: This study rapidly identified the pharmacodynamic markers (P-markers) against heart failure in American ginseng (AG) using the zebrafish model and metabolomics technology. These P-markers may provide new reference standards for quality control and new drug development of AG.
FRONTIERS IN PHARMACOLOGY
(2022)