Journal
ANALYTICA CHIMICA ACTA
Volume 1096, Issue -, Pages 44-52Publisher
ELSEVIER
DOI: 10.1016/j.aca.2019.10.052
Keywords
Horseradish peroxidase (HRP); Heated Au disk electrode; Electrocatalysis; Electrochemical sensor; H2O2
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Funding
- National Natural Science Foundation of China [21575026, 21874021]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R11]
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In this paper, horseradish peroxidase (HRP) was successfully immobilized on heated Au disk electrode (HAuDE) by biotin-streptavidin specific interaction through HS-ssDNA-biotin self-assembled on HAuDE for investigation the electrocatalytic activity of HRP. With elevated electrode temperature, the significant temperature effect of the electrocatalytic activity of HRP for H2O2 reduction was demonstrated by using this bio-sensing platform. With an electrode temperature of 40 degrees C, a detection limit of 1.5 x 10(-6) mol L-1 for H2O2 reduction could be obtained, which was more than one magnitude lower than that with an electrode temperature of 0 degrees C. Because HRP can be widely used as an enzyme label for amplification detection, this sensing platform can be broadly applied to analytical chemistry such as nucleic acid detection, and aptamer-based biosensors. (C) 2019 Elsevier B.V. All rights reserved.
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