Article
Parasitology
Patrick J. Skelly, Akram A. Da'dara
Summary: This article reviews the protein composition of schistosome secretions and highlights their impact on host immune responses. Some of these proteins have potential therapeutic applications in autoimmune, inflammatory, and non-parasitic diseases.
Article
Multidisciplinary Sciences
Lily Tran, Hayley Toet, Travis Beddoe
Summary: Fasciola hepatica, commonly known as liver flukes, is a significant parasitic disease affecting humans and livestock worldwide. Drug resistance has become a global issue, necessitating the implementation of an integrated parasite management program. A rapid DNA test using loop-mediated isothermal amplification was developed for the detection of F. hepatica from faecal and water samples, enabling real-time decision making to control parasite prevalence.
Article
Parasitology
Anthony Stumbo, Robert Poulin, Brandon Ruehle
Summary: Parasites can affect the visual system of fish, even without causing obvious pathology. When exposed to flashes of light, the intensity of the parasite is positively correlated with c-Fos gene expression, potentially causing difficulties for fish in distinguishing external environmental features.
Article
Agriculture, Dairy & Animal Science
Chairat Tantrawatpan, Wanchai Maleewong, Tongjit Thanchomnang, Warayutt Pilap, Takeshi Agatsuma, Ross H. Andrews, Paiboon Sithithaworn, Weerachai Saijuntha
Summary: This study aimed to characterize the intron regions of taurocyamine kinase gene (TK) in Opisthorchis viverrini and Clonorchis sinensis, and investigate their genetic variation and population genetics. The researchers found high nucleotide and haplotype diversity in O. viverrini samples, and observed heterozygous patterns specifically in humans with C. sinensis. They suggested that TkD1Int5 could be a high polymorphic genetic marker for further population genetic investigations of these liver flukes and related species.
Article
Chemistry, Organic
Arun K. Ghosh, Monika Yadav
Summary: Nirmatrelvir (Paxlovid) is an FDA approved drug that targets SARS-COV-2 3CLprotease. An optically active synthesis of nirmatrelvir was developed to avoid epimerization, which is a crucial step in the synthesis process. A ZnCl2-mediated direct N-trifluroacetylation method was utilized to overcome the epimerization problem and successfully synthesize nirmatrelvir derivatives without significant epimerization.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Callum Stewart-McGuinness, Christopher Platt, Matiss Ozols, Brian Goh, Tamara W. Griffiths, Michael J. Sherratt
Summary: This study used proteomics approaches to comprehensively analyze proteases and protease inhibitors in human skin, identifying various types of P/PIs and their distribution in normal and diseased skin. The results showed significant differences in the characteristics of P/PIs in different skin disease groups, suggesting a potential role of P/PIs in disease progression and providing important information for further research on the pathogenesis of skin diseases.
Article
Multidisciplinary Sciences
P. -T. Kao, H. Fleming, H. Warren, T. Darch, S. P. McGrath, H. L. Buss, M. R. F. Lee
Summary: The effect of organic and inorganic minerals (Se, Zn, Cu and Mn) on the partitioning and composition of micronutrients in sheep urine and faeces was studied. The form of the supplemented minerals did not affect the distribution and concentration of micronutrients in excreta, but the doses tested may alter the ratios of Se:P and Se:S in faeces and Se:S in urine. Mineral supplements improved the retention of P in sheep and differences were observed in the recalcitrant fractions of Se, Zn, and Cu in faeces when different forms of minerals were offered. Further investigation is needed to understand the potential impact of these differences on micronutrient flux in pasture.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Pavla Bartosova-Sojkova, Jiri Kyslik, Gema Alama-Bermejo, Ashlie Hartigan, Stephen D. Atkinson, Jerri L. Bartholomew, Amparo Picard-Sanchez, Oswaldo Palenzuela, Marc Nicolas Faber, Jason W. Holland, Astrid S. Holzer
Summary: Cysteine protease inhibitors known as cystatins play important roles in protein degradation and host immunity to parasites. This study identified diverse cystatins in early-emerging animal groups, including the discovery of a new subtype in parasitic cnidarians, the Myxozoa. The research explored the evolutionary history and lineage-specific adaptations of cystatins in early metazoans, shedding light on their potential roles in host-parasite interactions.
Article
Biochemistry & Molecular Biology
Miha Renko, Tanja Zupan, David F. Plaza, Stefanie S. Schmieder, Milica Perisic Nanut, Janko Kos, Dusan Turk, Markus Kuenzler, Jerica Sabotic
Summary: This article introduces a new family of fungal protease inhibitors called cocaprins, which can inhibit both cysteine and aspartic proteases. Cocaprins are small proteins with specific structure and biological functions, derived from the mushroom Coprinopsis cinerea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Richard Lalor, Krystyna Cwiklinski, Nichola Eliza Davies Calvani, Amber Dorey, Siobhan Hamon, Jesus Lopez Corrales, John Pius Dalton, Carolina De Marco Verissimo
Summary: Fasciolosis caused by liver flukes is an important neglected parasitic disease with high transmissibility and adaptability. The parasite's ability to survive and reproduce within their mammalian host is mainly influenced by their high frequency of non-synonymous polymorphisms in genes and excretory-secretory molecules. Through various mechanisms, liver flukes can persist for years within their host and spread rapidly.
Article
Biochemistry & Molecular Biology
Ana J. F. C. Aguiar, Jaluza L. C. de Queiroz, Pedro P. A. Santos, Christina S. Camillo, Alexandre C. Serquiz, Izael S. Costa, Gerciane S. Oliveira, Ana F. T. Gomes, Lidia L. R. Matias, Rafael O. A. Costa, Thais S. Passos, Ana H. A. Morais
Summary: The study found that ECW nanoparticles were beneficial for liver injury caused by a high glycemic index diet, reducing blood glucose levels, lowering glutamic oxalacetic transaminase and alkaline phosphatase levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Mehdi Yoosefian, Maryam Zeraati Moghani, Alfredo Juan
Summary: Researchers have discovered a new human immunodeficiency virus (HIV) protease inhibitor called ATV7 through molecular docking and molecular dynamics simulation, which has better antiviral effects and inhibitory ability than the existing drug atazanavir.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Infectious Diseases
Holly M. Craven, Riccardo Bonsignore, Vasilis Lenis, Nicolo Santi, Daniel Berrar, Martin Swain, Helen Whiteland, Angela Casini, Karl F. Hoffmann
Summary: This study identified the presence of G-quadruplexes (G4s) in Schistosoma mansoni for the first time, potentially providing a new target for the development of novel treatments against parasitic diseases such as schistosomiasis. G4s were found to be enriched in the wnt signalling pathway, suggesting that G4-targeted compounds could be explored as novel anthelmintic agents for the treatment of schistosomiasis.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biology
Eman M. N. Shakir, Gabriel Rinaldi, Ruth S. Kirk, Anthony J. Walker
Summary: This study found that excretory-secretory products (ESPs) from mature Schistosoma mansoni can induce responses in the opposite sex worms, leading to activation of specific signaling pathways and promotion of stem cell proliferation, thereby modulating worm development and disease progression.
COMMUNICATIONS BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Yanfeng Wang, Juan Zhao, Yebo Gu, Haiping Wang, Muzhou Jiang, Shuxuan Zhao, Hong Qing, Junjun Ni
Summary: CatH is a lysosomal cysteine protease with a unique aminopeptidase activity that is extensively expressed in various organs. Its specific enzymatic activity has critical effects on cancer cells and brain diseases. This review summarizes the expression, maturation, and enzymatic properties of CatH, and discusses the potential of CatH inhibitors in disease therapy.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yong Cheng, Raina M. Borum, Alex E. Clark, Zhicheng Jin, Colman Moore, Pavla Fajtova, Anthony J. O'Donoghue, Aaron F. Carlin, Jesse Jokerst
Summary: Researchers have developed a dual-color probe for the simultaneous detection of the main protease (MPpro) and papain-like protease (PLpro) of SARS-CoV-2. This probe produces fluorescence from two different fluorophores, which can be used for rapid screening of antiviral inhibitors.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Zhicheng Jin, Justin Yeung, Jiajing Zhou, Yong Cheng, Yi Li, Yash Mantri, Tengyu He, Wonjun Yim, Ming Xu, Zhuohong Wu, Pavla Fajtova, Matthew N. Creyer, Colman Moore, Lei Fu, William F. Penny, Anthony J. O'Donoghue, Jesse Jokerst
Summary: This study reports a divalent substrate that can interact with M-pro, facilitating the rapid and visual detection of SARS-CoV-2 coronavirus in COVID-19. The method shows comparable sensitivity to other reported methods and exhibits high specificity to M-pro.
CHEMISTRY OF MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Jakub Benysek, Michal Busa, Petra Rubesova, Jindrich Fanfrlik, Martin Lepsik, Jiri Brynda, Zuzana Matouskova, Ulrike Bartz, Martin Horn, Michael Gutschow, Michael Mares
Summary: This study investigates the binding mechanism of two model cyanohydrazide inhibitors with Cathepsin K (CatK) and reveals that the extraordinary potency of Gu2602 is favored by its conformational flexibility. The study also demonstrates the effectiveness of cyanohydrazides in targeting mature CatK in osteosarcoma cells and inhibiting the autoactivation of CatK. These findings provide important structural insights for the design of cyanohydrazide inhibitors as potential drugs.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Anneliese S. Ashhurst, Arthur H. Tang, Pavla Fajtova, Michael C. Yoon, Anupriya Aggarwal, Max J. Bedding, Alexander Stoye, Laura Beretta, Dustin Pwee, Aleksandra Drelich, Danielle Skinner, Linfeng Li, Thomas D. Meek, James H. McKerrow, Vivian Hook, Chien-Te Tseng, Mark Larance, Stuart Turville, William H. Gerwick, Anthony J. O'Donoghue, Richard J. Payne
Summary: The study identifies cathepsin L as a key protease used by coronaviruses for cell entry and a potential drug target for antiviral treatment against SARS-CoV-2. The researchers discovered that gallinamide A and synthetic analogues are potent inhibitors of cathepsin L, showing promising antiviral activity against SARS-CoV-2. The combination with a TMPRSS2 inhibitor further enhances the antiviral effect.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yong Cheng, Alex E. Clark, Jiajing Zhou, Tengyu He, Yi Li, Raina M. Borum, Matthew N. Creyer, Ming Xu, Zhicheng Jin, Jingcheng Zhou, Wonjun Yim, Zhuohong Wu, Pavla Fajtova, Anthony J. O'Donoghue, Aaron F. Carlin, Jesse Jokerst
Summary: This study reports a responsive, mitochondrial-targeting, modular-peptide-conjugated probe for selective imaging and inhibition of SARS-CoV-2-infected cells through enzyme-instructed self-assembly and aggregation-induced emission effect. This strategy may have value for selective detection and treatment of SARS-CoV-2-infected cells.
Article
Chemistry, Analytical
Ming Xu, Jiajing Zhou, Yong Cheng, Zhicheng Jin, Alex E. Clark, Tengyu He, Wonjun Yim, Yi Li, Yu-Ci Chang, Zhuohong Wu, Pavla Fajtova, Anthony J. O'Donoghue, Aaron F. Carlin, Michael D. Todd, Jesse Jokerst
Summary: A novel self-immolative fluorescent probe (MP590) has been developed for the highly selective and sensitive detection of SARS-CoV-2 main protease (M-pro). This probe can measure and monitor M-pro activity and quantitatively evaluate M-pro inhibition in infected cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Carina Lemke, Adela Jilkova, Dominic Ferber, Annett Braune, Anja On, Patrick Johe, Alena Zikova, Tanja Schirmeister, Michael Mares, Martin Horn, Michael Gutschow
Summary: We developed a bimodal rhodesain probe that can detect and quantify rhodesain with high sensitivity, and used it for inhibitor screening. This probe provides a new tool for studying Trypanosoma pathobiochemistry and antitrypanosomal drug discovery.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Medicinal
Lucianna H. Santos, Thales Kronenberger, Renata G. Almeida, Elany B. Silva, Rafael E. O. Rocha, Joyce C. Oliveira, Luiza Barreto, Danielle Skinner, Pavla Fajtova, Miriam A. Giardini, Brendon Woodworth, Conner Bardine, Andre Luiz Lourenco, Charles S. Craik, Antti Poso, Larissa M. Podust, James H. McKerrow, Jair L. Siqueira-Neto, Anthony J. O'Donoghue, Eufranio N. da Silva Junior, Rafaela S. Ferreira
Summary: This study identified novel naphthoquinonal derivatives as potential antiviral drugs for SARS-CoV-2 by using structure-based computational and biochemical methods. The compounds showed inhibitory effects on M-pro and PLpro in vitro, and demonstrated specificity.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Biochemistry & Molecular Biology
Michal Busa, Zuzana Matouskova, Pavla Bartosova-Sojkova, Petr Pachl, Pavlina Rezacova, Ramon Marc Eichenberger, Peter Deplazes, Martin Horn, Sasa Stefanic, Michael Mares
Summary: Fasciolosis is a parasitic disease caused by the liver fluke Fasciola hepatica and has become an emerging human disease. The protein FhCyLS-2 from F. hepatica is a member of the cystatin superfamily and has characteristics of both intracellular stefins and secreted true cystatins. It acts as a broad specificity inhibitor of cysteine cathepsins, playing a dual role in the regulation of exogenous and endogenous proteolysis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Public, Environmental & Occupational Health
Enoch Mensah Boateng, Jan Dvorak, Irene Ayi, Marta Chanova
Summary: Schistosomiasis has been extensively studied in Ghana, but there are research and knowledge gaps in areas such as livestock schistosomiasis and genital schistosomiasis. While studies have been conducted in Greater Accra and other regions, more efforts are needed to reduce the prevalence of the disease.
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Parasitology
Kristyna Peterkova, Jiri Vorel, Jana Ilgova, Pavel Ostasov, Pavla Fajtova, Lukas Konecny, Marta Chanova, Martin Kasny, Martin Horn, Jan Dvorak
Summary: This study investigates the proteases and inhibitors expressed by Schistosoma mansoni eggs using comparative transcriptomics. The findings suggest that these molecules may play important roles in egg tissue migration, stimulation of angiogenesis, and interaction with host blood and immunity.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
Article
Fisheries
Liliana Rojo Arreola, Eduardo Quiroz-Guzman, Jorge L. Caballero, Esther I. Ponce Garcia, Julio Humberto Cordova Murueta, Fernando Luis Garcia Carreno
Summary: In this study, crude enzymes from discarded P. vannamei were used to pretreat rice bran. The crude enzyme extract from P. vannamei contains proteases and amylases that can hydrolyze casein and rice bran starch, respectively, at a comparable rate to commercial enzymes. The enzymatic extracts obtained from shrimp discards are presented, contributing to the future sustainable use of agriculture, aquaculture, and fisheries resources.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Multidisciplinary Sciences
Stepanka Nedvedova, Florence Guilliere, Adriana Erica Miele, Francois-Xavier Cantrelle, Jan Dvorak, Olivier Walker, Maggy Hologne
Summary: This study presents a strategy to solve the structures of three alternatively spliced isoforms from the Schistoma mansoni MEG 2.1 family for the first time. The isoforms are hydrophobic, intrinsically disordered, and difficult to express in heterologous hosts. By using chemical synthesis and combining biophysical techniques with in silico molecular dynamics simulation, the structure of MEG 2.1 is successfully solved. This study provides crucial insights into this elusive and highly variable class of proteins.
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
