Article
Spectroscopy
Maciej Roman, Tomasz P. Wrobel, Agnieszka Panek, Czeslawa Paluszkiewicz, Wojciech M. Kwiatek
Summary: Modern techniques of radiotherapy, such as fractioned radiotherapy, require the application of low doses of ionizing radiation for effective treatment of cancer cells. Research shows that the response of cancer cells to radiation depends on both the dose and the time elapsed after irradiation, emphasizing the importance of considering both factors in studying the effects of ionizing radiation.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Ellas Spyratou, Agapi Ploussi, Panagiotis Alafogiannis, Hector Katifelis, Sotiria Apostolopoulou, Georgios Bagenakis, Spyridon Rammos, Ioannis Papagiannis, Maria Gazouli, Ioannis Seimenis, Alexandros G. Georgakilas, Efstathios P. Efstathopoulos
Summary: This study investigated the responsiveness of ferredoxin reductase (FDXR) on pediatric population exposed to ionizing radiation during pediatric interventional cardiology procedures. The results showed that the FDXR gene expression varied randomly with the effective dose within the radiation exposure range. Further studies are needed to determine whether FDXR gene expression is an effective biomarker for radiation exposure estimation in pediatric imaging.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Spectroscopy
Chen Shan, Lin Lan, Chen Jun, Liu Ying
Summary: In this study, Raman spectroscopy was used to investigate the biological effects of low-dose radiation. The results showed that low-dose X-ray radiation induced changes in the DNA level of cells, as well as cell cycle arrest and reduced cell migration. Raman spectroscopy can be employed as a tool for detecting and monitoring the early biological effects of low-dose radiation.
SPECTROSCOPY AND SPECTRAL ANALYSIS
(2023)
Article
Spectroscopy
Pedro Arthur Augusto de Castro, Derly Augusto Dias, Matheus del-Valle, Marcelo Noronha Veloso, Elizabeth Sebastiana Ribeiro Somessari, Denise Maria Zezell
Summary: This study characterizes the bone dose response to ionizing radiation using bovine femurs, and the combination of FTIR spectroscopy and PLS-DA allows for detection of the effects of ionizing radiation on biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Multidisciplinary Sciences
Wen Chen, Philippe Roelli, Huatian Hu, Sachin Verlekar, Sakthi Priya Amirtharaj, Angela Barreda, Tobias J. Kippenberg, Miroslavna Kovylina, Ewold Verhagen, Alejandro Martinez, Christophe Galland
Summary: Coherent upconversion of terahertz and mid-infrared signals into visible light was achieved using a plasmonic nanocavity hosting a few hundred molecules, demonstrating significant enhancement in upconversion efficiency per molecule. The results showcase that molecular cavity optomechanics is a flexible paradigm for frequency conversion leveraging tailorable molecular and plasmonic properties.
Article
Biochemical Research Methods
Christian Harry Allen, Robyn Skillings, Duale Ahmed, Sarita Cuadros Sanchez, Kaitlyn Altwasser, George Hilan, William G. Willmore, Vinita Chauhan, Edana Cassol, Sangeeta Murugkar
Summary: This study used stimulated Raman scattering (SRS) microscopy to investigate the radiobiological response in human breast cancer cells. The results show that radiation leads to an increase in lipid droplet area, total lipid and protein content per cell, as well as changes in cellular morphology. This pilot study demonstrates the potential of SRS imaging for studying radiation-induced changes in cancer cells.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Biochemistry & Molecular Biology
Eric Boateng Osei, Liliia Paniushkina, Konrad Wilhelm, Juergen Popp, Irina Nazarenko, Christoph Krafft
Summary: Extracellular vesicles (EVs) ranging in size from 60 to 800 nm were isolated from plasma of control and prostate cancer patients using differential centrifugation and ultrafiltration techniques. Spontaneous Raman and surface-enhanced Raman spectroscopy (SERS) were employed to identify fractions that best discriminate between control and cancer patients, with results showing that EV12 and EV120 fractions provide the most effective separation.
Article
Biochemistry & Molecular Biology
Maria Grazia Andreassi, Nadia Haddy, Mats Harms-Ringdahl, Jonica Campolo, Andrea Borghini, Francois Chevalier, Jochen M. Schwenk, Brice Fresneau, Stephanie Bolle, Manuel Fuentes, Siamak Haghdoost
Summary: The Health Effects of Cardiac Fluoroscopy and Modern Radiotherapy (photon and proton) in Pediatrics (HARMONIC) is a five-year project funded by the European Commission with the aim of improving understanding of long-term ionizing radiation risks for pediatric patients. This paper provides a detailed overview of the project's rationale, design, and methods for the biological aspect, with objectives to understand the molecular pathways and identify potential biomarkers involved in long-term health risks. Biological samples will be collected at three time points, and state-of-the-art analytical methods will be used to assess biomarker levels and explore proteomics and miRNA transcriptomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Hadis Rostamabadi, Ilkem Demirkesen, Bengi Hakgueder Taze, Asli Can Karaca, Mehvish Habib, Kulsum Jan, Khalid Bashir, Monica R. Nemtanu, Rosana Colussi, Seid Reza Falsafi
Summary: The challenges in the food/non-food applications of starch stem mainly from its low stability under harsh processing conditions, excessive retrogradability, and limited applicability. These drawbacks have been addressed through starch modification. Due to the increasing concerns about the potential side effects of chemical modification, scientists have turned their attention to the development of physical modification techniques. Ionizing and non-ionizing radiations have been introduced as potent physical strategies to modify starch, providing new outstanding attributes to the modified product. Ionizing radiations can induce depolymerization or cross-linking/grafting reactions in the starch medium, while non-ionizing radiations can modify starch attributes by altering granule morphology/architecture and reorienting/rearranging the molecular order of starch amorphous/crystalline fractions.
Article
Oncology
N. Ari Wijetunga, Alexander G. Goglia, Nils Weinhold, Michael F. Berger, Michael Cislo, Daniel S. Higginson, Kiana Chabot, Ahmed M. Osman, Lauren Schaff, Elena Pentsova, Alexandra M. Miller, Simon N. Powell, Adrienne Boire, Jonathan T. Yang
Summary: This study analyzed the genetic characteristics and changes before and after proton craniospinal irradiation (pCSI) in patients with solid tumor leptomeningeal metastasis (LM) to explore their relationship with clinical response. The results showed unique mutations in the cell-free DNA of cerebrospinal fluid (CSF) before and after pCSI, and lower genomic diversity was associated with extended survival after pCSI.
CLINICAL CANCER RESEARCH
(2023)
Review
Oncology
Chenbei Li, Chengyao Feng, Ruiling Xu, Buchan Jiang, Lan Li, Yu He, Chao Tu, Zhihong Li
Summary: Although the survival rate of pediatric cancer has improved, it is still a significant cause of death among children. Raman spectroscopy, a non-destructive analytical technique, has shown promise in the diagnosis, prognosis, and treatment of pediatric cancers. Studies have demonstrated high accuracy and specificity, and the potential future application of combining Raman spectroscopy with deep learning is discussed.
FRONTIERS IN ONCOLOGY
(2023)
Article
Orthopedics
Ashley B. Anderson, Julio A. Rivera, Edmund P. Mullin, Collin J. Harrington, Benjamin K. Potter, Jonathan A. Forsberg, Scott M. Tintle
Summary: Patients with complex polytrauma in military and civilian settings are exposed to substantial diagnostic medical radiation, increasing the risk of subsequent malignancy. Currently, there is no coordinated effort to track radiation exposure or long-term surveillance for such patients. This study aims to quantify radiation exposure for combat-wounded servicemembers and determine its correlation with the Injury Severity Score (ISS).
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
(2023)
Article
Chemistry, Physical
A. Abouelsayed, Gamal S. El-Bahy, Abdelrazek B. Abdelrazzak
Summary: Proteins are an important component of plasma membrane and play a crucial role in various membrane functions. Modifying the protein conformation can affect its functionality. In this study, we observed the induction of radioadaptation in the kidneys of low-dose irradiated rats, resulting in diminishment of protein structural alterations.
JOURNAL OF MOLECULAR STRUCTURE
(2024)
Article
Spectroscopy
Camila Ribeiro Daniel, Marcos Tadeu Tavares Pacheco, Ana Mara Ferreira Lima, Pedro Luiz de Brito, Landulfo Silveira Jr
Summary: This study used Raman spectroscopy to analyze biochemical changes in blood cell samples and discriminate between normal, leukemic, and non-leukemic subjects. Significant differences were observed in various bands corresponding to nucleic acids, amino acids, proteins, β-carotene, and cytochrome c, with higher intensity in normal samples. Discriminant analysis accurately classified the spectra into different groups, with higher accuracy for normal versus cancer samples compared to leukemic versus non-leukemic samples.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Polymer Science
Joshua Moncada, Mark D. Dadmun
Summary: This study investigates the changes in polymer structure after exposure to ionizing radiation using synchrotron x-rays. The results show that the ionizing radiation induces a reaction cascade that continues long after removal of the x-ray beam, and the reaction rate is controlled by the exposure time. These findings provide insights into the stability of conjugated polymers in the presence of reactive diluents and ionizing radiation, and offer new possibilities for forming graft copolymers.
POLYMER DEGRADATION AND STABILITY
(2022)
Review
Medicine, Research & Experimental
Raluca Petea-Balea, Manuela Lenghel, Horatiu Rotar, Cristian Dinu, Simion Bran, Onisor Florin, Rares Roman, Simona Senila, Csaba Csutak, Ciurea Anca
Summary: Ultrasound is the first-line imaging technique, but may not fully assess the extent of vascular anomalies in some cases. Magnetic resonance imaging with DCE-MRI allows accurate evaluation of the extent and anatomical relationship of vascular anomalies, as well as providing hemodynamic characterization, aiding in therapy selection and prognosis assessment.
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Radu Nicolae Revnic, Gabriela Fabiola Stiufiuc, Valentin Toma, Anca Onaciu, Alin Moldovan, Adrian Bogdan Tigu, Eva Fischer-Fodor, Romulus Tetean, Emil Burzo, Rares Ionut Stiufiuc
Summary: We have developed a simple and reproducible method for the synthesis of anisotropic silver nanostars (AgNS) using only trisodium citrate as a reducing and stabilizing agent. These nanostars demonstrate high efficiency as SERS substrates, even with near-infrared excitation laser. The key elements of this synthesis procedure include rapid hydrothermal synthesis and a cooling down procedure using a water bath.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Vlad Cristian Munteanu, Raluca Andrada Munteanu, Diana Gulei, Radu Marginean, Vlad Horia Schitcu, Anca Onaciu, Valentin Toma, Gabriela Fabiola Stiufiuc, Ioan Coman, Rares Ionut Stiufiuc
Summary: Research on biofluids using Raman and SERS has shown promise in identifying cancer and other disorders. Current studies focus on specific illnesses, and further research and standardization of experimental conditions are needed. This study successfully applied combined SERS multivariate analysis to diagnose prostate cancer, achieving positive results. The method shows higher specificity compared to the PSA test.
Review
Health Care Sciences & Services
Oana Almasan, Daniel-Corneliu Leucuta, Mihaela Hedesiu
Summary: We found an association between NLR, LMR, and PLR and periodontitis, which could be considered as emerging blood cell count inflammatory biomarkers that may contribute to understanding the connection between periodontitis and systemic imbalances, as well as to periodontitis prognosis and grading.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Dentistry, Oral Surgery & Medicine
Sorana Muresanu, Oana Almasan, Mihaela Hedesiu, Laura Diosan, Cristian Dinu, Reinhilde Jacobs
Summary: This study provides a comprehensive review of the literature on the use of artificial intelligence (AI) in dental and maxillofacial cone beam computed tomography (CBCT), with the aim of assisting future researchers and dental professionals. The study found that AI models, particularly deep learning models, are commonly used in this field. The applications of AI have the potential to improve oral healthcare quality, promote personalized dentistry, and expedite dental procedures.
Article
Chemistry, Multidisciplinary
Adrian Bartos, Ioana Iancu, Lidia Ciobanu, Anca Onaciu, Cristian Moldovan, Alin Moldovan, Radu Cristian Moldovan, Adrian Bogdan Tigu, Gabriela Fabiola Stiufiuc, Valentin Toma, Cornel Iancu, Nadim Al Hajjar, Rares Ionut Stiufiuc
Summary: This study proposes a nanotechnology-based formulation of sorafenib-loaded nanoliposomes to overcome the pharmacokinetic challenges of sorafenib. The liposomal hybrid formulations were characterized and their therapeutic efficacy was evaluated on normal and hepatocarcinoma cell lines.
Article
Biochemistry & Molecular Biology
Lucia Ruxandra Tefas, Ioana Toma, Alina Sesarman, Manuela Banciu, Ancuta Jurj, Ioana Berindan-Neagoe, Lucia Rus, Rares Stiufiuc, Ioan Tomuta
Summary: Colorectal cancer is a major cause of illness and death worldwide, and targeted elimination of both cancer stem cells (CSCs) and non-CSCs is essential for successful treatment. This study developed and optimized a liposomal drug delivery system for co-loading the anti-CSC drug salinomycin (SAL) and the conventional anticancer drug gemcitabine (GEM). The co-loaded liposomes demonstrated sustained release of both drugs and superior in vitro cytotoxic effects compared to single GEM treatment, indicating that this combination therapy may be a promising strategy for treating colorectal cancer.
JOURNAL OF LIPOSOME RESEARCH
(2023)
Review
Dentistry, Oral Surgery & Medicine
Oana Almasan, Mihaela Hedesiu, Mihaela Baciut, Smaranda Buduru, Cristian Dinu
Summary: This study aimed to conduct a systematic review on temporomandibular joint (TMJ) psoriatic arthritis (PsA). A total of 23 publications with 151 TMJ PsA patients were included. Clinical symptoms of TMJ PsA included TMJ pain and sounds, limited range of jaw movements, preauricular swelling, malocclusion, headache, tinnitus, neck stiffness, and altered dietary function. Evaluation of TMJ included MRI, CT, and ultrasonography. Conservative therapy showed significant improvement in TMJ function.
JOURNAL OF ORAL REHABILITATION
(2023)
Article
Chemistry, Analytical
Anca Onaciu, Valentin Toma, Cristian Moldovan, Adrian Bogdan Tigu, Diana Cenariu, Carina Culic, Rares Mario Borsa, Luca David, Gabriela Fabiola Stiufiuc, Romulus Tetean, Ciprian Tomuleasa, Rares Ionu Stiufiuc
Summary: DNA methylation is a critical epigenetic marker in cancer development, but there is a lack of experimental methods to prove nanoscale modifications. Raman and surface-enhanced Raman scattering (SERS) have become promising techniques for investigating nanoscale DNA modifications. In our study, we used Raman/SERS to differentiate normal and leukemia DNA samples and evaluate the effects of 5-azacytidine treatment on leukemia cells.
Article
Health Care Sciences & Services
Cosmin Ioan Faur, Cristian Dinu, Valentin Toma, Anca Jurj, Radu Marginean, Anca Onaciu, Rares Calin Roman, Carina Culic, Magdalena Chirila, Horatiu Rotar, Alexandra Falamas, Gabriela Fabiola Stiufiuc, Mihaela Hedesiu, Oana Almasan, Rares Ionut Stiufiuc
Summary: Raman spectroscopy is a non-invasive, rapid, and reliable method for cancer detection, and it has recently shown great potential in identifying disease-specific markers. In this study, vibrational spectra of salivary exosomes from oral and oropharyngeal squamous cell carcinoma patients and healthy controls were recorded using surface enhancement Raman spectroscopy (SERS). The method's ability to discriminate between malignant and non-malignant samples was assessed using chemometric analysis, with a discrimination sensitivity of up to 79.3%.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Rares-Mario Borsa, Valentin Toma, Anca Onaciu, Cristian-Silviu Moldovan, Radu Marginean, Diana Cenariu, Gabriela-Fabiola Stiufiuc, Cristian-Mihail Dinu, Simion Bran, Horia-Octavian Opris, Sergiu Vacaras, Florin Onisor-Gligor, Dorin Sentea, Mihaela-Felicia Baciut, Cristina-Adela Iuga, Rares-Ionut Stiufiuc
Summary: Cancer remains a major challenge in medical practice. Oral cancer, in particular, has a significant impact due to its visibility, leading to social stigma and increased anxiety. New early diagnosis methods are needed to improve treatment efficiency and patients' quality of life.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Bogdan Adrian Buhas, Valentin Toma, Nicolae Crisan, Guillaume Ploussard, Teodor Andrei Maghiar, Rares-Ionut Stiufiuc, Constantin Mihai Lucaciu
Summary: This study investigated the use of serum label-free SERS combined with two multivariate analysis tests for the discrimination of RCC cancer patients from healthy individuals. The results showed that the combination of LDA-PCA and SVM methods can effectively distinguish RCC patients from healthy controls. This technique holds great promise for non-invasive early detection of RCC.
