Article
Biotechnology & Applied Microbiology
Neelam Mishra, Kavita Rana, Siva Deepthi Seelam, Rakesh Kumar, Vijyendra Pandey, Bharathi P. Salimath, Dayanand Agsar
Summary: Pseudomonas aeruginosa DNM50 was identified as a biosurfactant-producing bacterium with 12 different congeners of rhamnolipid identified through structural characterization. The rhamnolipid showed radical scavenging activity with an IC50 value of 101.8 μg/ml and exhibited cytotoxic activity against MDA-MB-231 breast cancer cell line with a low IC50 of 0.05 μg/ml at 72 h of treatment. This suggests that DNM50RL may have potential in breast cancer treatment by targeting inhibition of p38MAPK.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Chun-Wen Cheng, Gwo-Tarng Sheu, Jing-Shiuan Chou, Pei-Han Wang, Yu-Chun Cheng, Chane-Yu Lai
Summary: The study demonstrated that soluble PM2.5 from building demolition sites can promote the survival and metastasis of triple-negative breast cancer cells, with increased cell viability, migration capacity, and activation of malignant signaling pathways in cancer cells exposed to the particulates.
Article
Biochemistry & Molecular Biology
Sara Di Carlo, Georg Haecker, Ian E. Gentle
Summary: GM-CSF promotes the ubiquitination and enhanced processing of IL-1 beta through suppression of antioxidant responses, leading to its pro-inflammatory effect.
Article
Biophysics
Rahman Hallaj, Zhaleh Ghafary, Osama kamal Mohammed, Raheleh Shakeri
Summary: The study developed an electrochemical biosensor for detecting the endogenous protein of breast cancer cells using RNA-cleaving DNAzymes. Thionine-modified gold nanoparticles and modified magnetic nanoparticles were used to prepare a probe, which was then pulled to the electrode surface with the help of a magnetic field. The presence of gold nanoparticle-thionine hybrid as an electroactive label ensured strong detection signals. The biosensor showed a linear range of (1.0E-06 to 1.0E+01) pg/ml for the protein marker and a detection limit of (1.0129E-07 pg/ml) using differential pulse voltammetry and electrochemical impedance spectroscopy (EIS).
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Xingjian Zhang, Trevor Chan, Michael Mak
Summary: This study investigated the phenotypes of highly aggressive cancer cells in confined microenvironments, revealing two distinct migratory phenotypes in fast migrating single cells and an increase in protrusions at the leading edge of cells under geometric confinement. The findings elucidate the impact of geometric constraints on cell behavior and morphodynamics associated with different cell phenotypes.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kamylla Fernanda Souza de Souza, Danilo Tofoli, Indiara Correia Pereira, Kelly Juliana Filippin, Ana Tereza Gomes Guerrero, Edgar Julian Paredes-Gamero, Maria de Fatima Cepa Matos, Walmir Silva Garcez, Fernanda Rodrigues Garcez, Renata Trentin Perdomo
Summary: The study isolated three compounds from the leaves of Aniba heringeri, with compound 3 showing strong cytotoxicity against breast cancer cells and a high selective index. This compound exhibited promising apoptosis induction effects on triple-negative breast cancer cells, demonstrating potential as a prototype for targeting TNBC cells that are usually resistant to conventional therapies.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Florian Keller, Roman Bruch, Franziska Clauder, Mathias Hafner, Ruediger Rudolf
Summary: The study found that extracellular matrix components play a significant role in regulating BSP expression, suggesting mechanisms for the formation of bone-associated metastasis in breast cancer that may involve local control of BSP levels through extracellular matrix degradation and release of growth factors.
Article
Cell Biology
Marco A. Lacerda-Abreu, Thais Russo-Abrahao, Raissa Leite Tenorio Aguiar, Robson de Queiroz Monteiro, Franklin D. Rumjanek, Jose R. Meyer-Fernandes
Summary: Breast cancer is associated with genetic mutations that lead to uncontrolled growth of breast cells. Tumor cells have rewired energy metabolism compared to normal cells, with inorganic phosphate playing a crucial role in cancer cell proliferation. Understanding the activity of ectophosphatase can provide insights into cancer cell energy requirements and growth mechanisms.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Biology
Fatma Mousa AlGhalban, Amir Ali Khan, Muhammad Nasir Khan Khattak
Summary: The latex of Ficus salicifolia and Ficus carica exhibit anti-proliferative and anti-metastatic effects in TNBC cells. They differ in the level of toxicity and molecular mechanisms involved in their anti-cancer activity.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
C. Cantonero, P. J. Camello, G. M. Salido, J. A. Rosado, P. C. Redondo
Summary: TMEM97 is involved in the proliferation of breast cancer cells by regulating calcium influx and promoting cholesterol uptake. It acts by influencing the interaction between SOCE calcium channel and cholesterol in cancer cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Oncology
Rahil Ghanbarnasab Behbahani, Amir Danyaei, Ali Teimoori, Mohammad Javad Tahmasbi, Niloofar Neisi
Summary: This study investigated the synergistic effects of OPN gene knockout and radiotherapy in MDA-MB-231 breast cancer cells using CRISPR/Cas9 technique. The results showed that the combination of OPN gene knockout and radiotherapy promoted cell apoptosis, suppressed downstream gene expression, reduced cell viability, and inhibited cell cycle progression. Additionally, this approach substantially altered DNA damage responses.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Tina Rabizadeh, Reyhaneh Varshochian, Athar Mahdieh, Mahsa Rezaei, Negin Pazouki, Mahsa Zardkanlou, Shiva Irani, Rassoul Dinarvand
Summary: In this study, polydopamine-coated superparamagnetic iron oxide nanoparticles were used to deliver the immunomodulatory drug teriflunomide to breast cancer cells. The nanoparticles showed promising potential by increasing cytotoxicity and gene expression in the cells. The study suggests that the designed teriflunomide-loaded nanoparticles could be considered for future breast cancer therapy research.
JOURNAL OF CLUSTER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hadi Chavoshi, Mahsa Taheri, Murphy Lam Yim, Mehdi Sabzichi
Summary: Triple-negative breast cancer (TNBC) is a highly lethal subtype of breast cancer. Crocin-loaded liposomes showed enhanced cytotoxicity when combined with chemotherapy drug doxorubicin (DOX) in MDA-MB-231 cells. The liposome formulation had monodisperse distribution with an average size of 80 nm, about 70% encapsulation efficiency, and 11% loading capacity. The study suggested that crocin-loaded liposomes can be a promising approach against tumor cells, especially when combined with DOX.
PROCESS BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Ling Chen, Li-Quan Ren, Zhong Liu, Xin Liu, Han Tu, Xu-Ying Huang
Summary: This study investigated the anti-cancer mechanism of Schisandrin A in breast cancer through bio-informatics analysis and in vitro experiments. The results showed that SchA could promote cell migration and inhibit apoptosis by regulating specific targets, making it a potential adjuvant treatment for breast cancer.
Article
Biochemistry & Molecular Biology
Ha Thi Thu Do, Jungsook Cho
Summary: The study revealed that XCL1 enhances cell migration and epithelial-mesenchymal transition in breast cancer cells MDA-MB-231 and SK-BR-3 through the activation of ERK/HIF-1α/EMT signaling pathway. The XCL1-XCR1 interaction may serve as a specific target for preventing breast cancer cell migration and metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Mohammad Qaddoumi, Prashantha Hebbar, Mohamed Abu-Farha, Aseelah Al Somaly, Motasem Melhem, Fadi Al-Kayal, Irina AlKhairi, Preethi Cherian, Muath Alanbaei, Fahd Al-Mulla, Jehad Abubaker, Thangavel Alphonse Thanaraj
Summary: N-Acetylgalactosaminyltransferase 2 (GALNT2) is associated with serum lipid levels, insulin resistance, and adipogenesis. This study investigated the association between GALNT2 rs4846914 variant and plasma levels of ANGPTL proteins, apolipoproteins, lipids, and obesity traits in individuals of Arab ethnicity. The rs4846914_G allele of GALNT2 was associated with increased ANGPTL3, higher BMI and weight, increased ApoC1 levels, and reduced HDL levels. Individuals carrying the GG genotype showed decreased HDL and increased BMI, WC, ApoC1, and TG. GALNT2 is a potential link between lipid metabolism and obesity.
Article
Immunology
Amal Hasan, Hossein Arefanian, Arshad Mohamed Channanath, Irina AlKhairi, Preethi Cherian, Sriraman Devarajan, Thangavel Alphonse Thanaraj, Mohamed Abu-Farha, Jehad Abubaker, Fahd Al-Mulla
Summary: COVID-19 vaccines can cause elevation of coagulation factors and antithrombin, with differences observed between different vaccines. The clinical significance of the disruption of coagulation homeostasis remains unclear and requires further research.
Article
Cell Biology
Mohammed J. A. Haider, Zahraa Albaqsumi, Fahd Al-Mulla, Rasheed Ahmad, Fatema Al-Rashed
Summary: This study evaluated the expression and function of Dectin-2 in peripheral blood mononuclear cells (PBMCs) from diabetic patients and found that Dectin-2 expression was significantly increased in the monocytes of diabetic patients compared with non-diabetic controls. The activation of Dectin-2 was associated with markers of glucose homeostasis and elevated levels of inflammatory markers in PBMCs from diabetic patients.
Review
Biochemistry & Molecular Biology
Abdulmohsen Alterki, Mohamed Abu-Farha, Eman Al Shawaf, Fahd Al-Mulla, Jehad Abubaker
Summary: Obstructive sleep apnoea (OSA) is a prevalent but underdiagnosed disorder that disrupts the circadian rhythm and is characterized by frequent interruptions in breathing during sleep, leading to systemic consequences. OSA is a well-recognized risk factor for cardiovascular and cerebrovascular diseases, and it is also linked to obesity. The pathogenesis of both OSA and obesity involve oxidative stress, inflammation, and metabolic dysregulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ashraf Al Madhoun, Shihab Kochumon, Dania Haddad, Reeby Thomas, Rasheeba Nizam, Lavina Miranda, Sardar Sindhu, Milad S. Bitar, Rasheed Ahmad, Fahd Al-Mulla
Summary: Obesity is associated with chronic low-grade inflammation. Obese individuals have higher levels of CAV1 protein in adipose tissues. This study found a positive correlation between CAV1 gene expression and TNF-a. and NF -KB gene expression in obese adipose tissues.
Article
Biochemistry & Molecular Biology
Nadeem Akhter, Ajit Wilson, Hossein Arefanian, Reeby Thomas, Shihab Kochumon, Fatema Al-Rashed, Mohamed Abu-Farha, Ashraf Al-Madhoun, Fahd Al-Mulla, Rasheed Ahmad, Sardar Sindhu
Summary: Obesity and metabolic syndrome are associated with metabolic inflammation, endotoxin, and free fatty acids. The study found that the interaction between metabolic stress and endoplasmic reticulum (ER) stress promotes inflammation and triggers TNF-alpha production through the ROS/CHOP/HIF-1 alpha and MAPK/NF-kappa B pathways. Antioxidants show therapeutic potential in inflammatory conditions involving metabolic/ER stresses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Anwar Mohammad, Eman Alshawaf, Hossein Arefanian, Sulaiman K. Marafie, Abbas Khan, Dong-Qing Wei, Fahd Al-Mulla, Jehad Abubaker
Summary: In this study, natural medicinal compounds were investigated as potential inhibitors of SARS-CoV-2-NSP3 macrodomain-I. Computational modeling and simulation tools were used to explore the structural dynamics and binding affinity of the compounds. Two significant hits were identified, with strong binding affinity and inhibitory potential. The findings provide a basis for novel drug development against SARS-CoV-2 and its variants.
Article
Nutrition & Dietetics
Rashed Alhammad, Mohamed Abu-Farha, Abdur Rahman, Thangavel Alphonse Thanaraj, Lemia Shaban, Reem Alsabah, Samar Hamad, Maha M. Hammad, Arshad Channanath, Fahd Al-Mulla, Jehad Abubaker
Summary: This study investigated the association between LRG1 and iron deficiency anemia markers in school-aged children. High LRG1 levels were observed in anemic children compared to non-anemic children. Significant correlations were found between LRG1 and various markers including Hb, albumin, iron, and RDW. This research provides valuable insights into the relationship between LRG1 and iron deficiency anemia in children.
Article
Cell Biology
Mohamed Abu-Farha, Dhanya Madhu, Prashantha Hebbar, Anwar Mohammad, Arshad Channanath, Sina Kavalakatt, Nada Alam-Eldin, Fatima Alterki, Ibrahim Taher, Osama Alsmadi, Mohammad Shehab, Hossein Arefanian, Rasheed Ahmad, Thangavel Alphonse Thanaraj, Fahd Al-Mulla, Jehad Abubaker
Summary: The ANGPTL8 R59W variant is associated with increased levels of TNF alpha, IL7, and NF-kappa B p65 activity. The weak transient binding of the ANGPTL8 R59W variant explains its regulatory role on the NF-kappa B pathway and inflammation.
Review
Cell Biology
Sulaiman K. Marafie, Fahd Al-Mulla
Summary: Post-translational modifications are crucial for regulating human diseases, and furin, a type of irreversible post-translational modifier, plays a role in the context of type 2 diabetes and the mTOR signaling pathway. Understanding the role of furin in diabetes and exploring its genetic variants is important for future therapeutic interventions.
Article
Biochemistry & Molecular Biology
Mohamed Abu-Farha, Shibu Joseph, Anwar Mohammad, Arshad Channanath, Ibrahim Taher, Fahd Al-Mulla, Muhammad Mujammami, Thangavel Alphonse Thanaraj, Jehad Abubaker, Anas M. Abdel Rahman
Summary: This study used metabolomics to investigate the impact of the ANGPTL8 R59W variant on metabolites in Arab individuals. The results showed significant associations between the variant and concentrations of lipid- and inflammation-related metabolites, highlighting the importance of this gene in lipid metabolism.
Article
Biochemistry & Molecular Biology
Zhi Liu, Aleksandar Krstic, Ashish Neve, Cristina Casalou, Nora Rauch, Kieran Wynne, Hilary Cassidy, Amanda McCann, Emma Kavanagh, Brendan McCann, Alfonso Blanco, Jens Rauch, Walter Kolch
Summary: KSR1 plays a critical role in mutant BRAF transformation, and its loss results in impaired cell proliferation and migration, cell cycle abnormalities, cellular senescence, and apoptosis. The study reveals that KSR1 directs ERK to phosphorylate substrates essential for cell survival, and its loss activates p38 MAPK pathway leading to cell cycle aberrations and senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell Biology
N. K. Aboud, L. Garcia-Gutierrez, W. Kolch, D. Matallanas
MOLECULAR BIOLOGY OF THE CELL
(2023)
Meeting Abstract
Cell Biology
N. K. Aboud, L. Garcia-Gutierrez, W. Kolch, D. Matallanas
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Biochemistry & Molecular Biology
Hana Th. Almajed, Mohamed Abu-Farha, Eman Alshawaf, Sriraman Devarajan, Zahra Alsairafi, Ashraf Elhelaly, Preethi Cherian, Irina Al-Khairi, Hamad Ali, Rose Mol Jose, Thangavel Alphonse Thanaraj, Ebaa Al-Ozairi, Fahd Al-Mulla, Abdulnabi Al Attar, Jehad Abubaker
Summary: This study found a significant increase in circulating ANGPTL8 levels in patients with diabetic nephropathy (DN), and the levels of ANGPTL8 were positively correlated with IGFBP-4 levels. This suggests a potential role of both ANGPTL8 and IGFBP-4 in the pathophysiology of DN. These findings highlight the significance of these biomarkers and suggest them as promising diagnostic molecules for the detection of DN.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)