Article
Polymer Science
Xiangsheng An, Shujun Duan, Zhicong Jiang, Sunan Chen, Wenxuan Sun, Xiaoyan Liu, Zhonghao Sun, Yinping Li, Mingyan Yan
Summary: The study investigated the cross-linking of tilapia collagen fibrillar gels (CFG) with chlorogenic acid (CGA) and procyanidin (PC), and found that PC cross-linking resulted in better properties, including higher cross-linking effect, denser fibril network, improved water absorption and retention capacity, mechanical properties, enzymatic resistance, as well as better antioxidant and antibacterial activity.
POLYMER DEGRADATION AND STABILITY
(2022)
Article
Chemistry, Applied
Jingjing Su, Yaqian Geng, Jinbo Yao, Yuan Huang, Junfu Ji, Fang Chen, Xiaosong Hu, Lingjun Ma
Summary: Non-enzymatic browning induced by polyphenol oxidation is a significant concern during the processing and storage of fruit and vegetable products. The study investigated the mechanism of non-enzymatic browning between catechin (CAT), chlorogenic acid (CQA), and their quinones in model systems during long-term storage. The results showed that CAT and catechin quinone (CATQ) were important precursors for browning, while CQA had a minor effect. The oxidation of CAT by CQAQ was faster than CAT autoxidation, and complex reactions between CATQ and CAT produced brown pigments during storage.
Article
Biochemistry & Molecular Biology
Bogumila Urbanska, Hanna Kowalska, Karolina Szulc, Malgorzata Ziarno, Irina Pochitskaya, Jolanta Kowalska
Summary: The content of polyphenols in chocolate depends on raw materials and manufacturing parameters. Research shows that chocolates made from unroasted cocoa beans have the highest polyphenol content. Both the temperature and time at the conching stage significantly affect the content of polyphenols and the rheological properties of chocolate milk mass.
Article
Chemistry, Medicinal
Abdulwahab Alqahtani, Diaa A. Marrez, Mohamed Aleraky, Nada A. Fagir, Omaish Alqahtani, Samir Othman, Mohamed A. El Raey, Hany G. Attia
Summary: Pathogenic microbes have developed antibiotic resistance worldwide, which is concerning. This study aimed to identify and isolate active components from Ficus retusa bark extract and assess their potential synergistic activity with tetracycline against certain pathogenic bacteria. The combination of catechin and chlorogenic acid with tetracycline significantly enhanced antibacterial action, suggesting their potential as innovative and safe antibiotics to combat microbial resistance.
Article
Plant Sciences
Ji-Hye Kim, Jong-Eun Han, Hosakatte Niranjana Murthy, Ja-Young Kim, Mi-Jin Kim, Taek-Kyu Jeong, So-Young Park
Summary: Sageretia thea was cultivated with the aim of increasing the production of phenolic compounds in plant cell suspension cultures. Optimum callus was induced and methyl jasmonate was found to be a suitable elicitor for phenolic accumulation in cell suspensions. The use of balloon-type bioreactors resulted in the highest biomass yields after four weeks of cultivation.
Article
Food Science & Technology
Emine Kucuker, Muttalip Gundogdu, Erdal Aglar, Ferhat Ogurlu, Tuncer Arslan, Ceyda Kizgin Ozcengiz, Onur Tekin
Summary: In this study, the effect of melatonin applications on peach fruits during cold storage was investigated. The results showed that melatonin treatment reduced weight loss and decay rate, maintained fruit firmness, and preserved the concentration of organic acids.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Nutrition & Dietetics
Joshua P. Nederveen, Alexander J. Mastrolonardo, Donald Xhuti, Alessia Di Carlo, Katherine Manta, Matthew R. Fuda, Mark A. Tarnopolsky
Summary: This study investigated the effects of a multi-ingredient nutritional supplement on metabolic function and weight loss. The results showed that after 12 weeks of supplementation, there were significant improvements in weight, body composition, and molecular markers of liver health and metabolism.
Article
Chemistry, Medicinal
Shui Aoki, Hisae Oku, Fumika Kitagawa, Naoki Inoue, Chie Honda
Summary: This study demonstrated that the ethanol extract of Kummerowia striata has a significant effect on improving stagnant blood flow. Fourteen flavone and flavonol derivatives were isolated from K. striata, and three of them showed better improvement of blood flow compared to the positive control.
NATURAL PRODUCT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yashika Gandhi, Hemant Rawat, Daljeet Singh Dhanjal, Vijay Kumar, Vaibhav Charde, Hemant Soni, Sujeet K. Mishra, Gagandeep Singh, Simranjeet Singh, Preeti Sharma, Santosh K. Shakya, C. V. Narsimhaji, Ajay K. Meena, Arjun Singh, Ravindra Singh, Naryanam Srikanth, Rabinarayan Acharya
Summary: The leaves of Salix alba L. contain higher amounts of Salicin, Procyanidin B1, and Catechin compared to the stem bark, making them a potential substitute for the bark. They also exhibit significant sources of nutrients, phytochemicals, and medicinal properties. Further pharmacological and toxicological studies are needed to confirm their suitability.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Integrative & Complementary Medicine
Carla Marrassini, Elina Malen Saint Martin, Maria Rosario Alonso, Claudia Anesini
Summary: This study evaluated the anti-inflammatory and antioxidant activities of Urtica circularis extracts and identified two compounds that contribute to the biological activity. The ethanol extract of U. circularis was effective against inflammation, while the aqueous extract showed potential for treating oxidative stress-related diseases. The observed differences in activity between the extracts may be due to variations in chemical composition and/or synergistic effects between compounds.
BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS
(2023)
Article
Chemistry, Applied
Caiyun Li, Xuefeng Ding, Jie Li, Shoulei Yan
Summary: The effect and oxidation pathway of AA on the stability of Cat were investigated at different temperatures. The results showed that adding AA can enhance the stability of Cat, but excessive AA can promote the consumption of Cat.
Article
Food Science & Technology
Shaohua Liang, Quan Zhao, Xianzhi Wei, Cong Sun
Summary: In this study, chlorogenic oleate (CGO) was efficiently synthesized through acylation of chlorogenic acid (CGA) and oleoyl chloride. By optimizing the reaction conditions, the researchers successfully obtained a high purity product. The best synthesis conditions, including substrate molar ratio, adding method, reaction time, and temperature, were determined through experiments.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Shan Wang, Yue Li, Xiangyong Meng, Shangwei Chen, Dejian Huang, Yongmei Xia, Song Zhu
Summary: In this study, chlorogenic acid was acylated with different carbon chain lengths to produce derivatives with varying lipophilicities, which were found to have enhanced antioxidant activities in cellular antioxidant activity tests. The results indicated that the lipophilicities of the derivatives positively correlated with their digestive stabilities and cellular antioxidant activities. Among the derivatives, C12-CA exhibited the best antioxidant activity with a significantly lower effective dose compared to CA.
Article
Agriculture, Dairy & Animal Science
Huawei Liu, Xuemin Li, Shuyan Shi, Yingjun Zhou, Kai Zhang, Yang Wang, Jinshan Zhao
Summary: Chlorogenic acid (CGA) has positive effects on growth performance and intestinal health in oxidatively stressed broilers, acting through the activation of the autophagy-mediated Nrf2 pathway.
Article
Biochemistry & Molecular Biology
Keren Bindon, Song Qi, Stella Kassara, Luca Nicolotti, Alicia Jouin, Maggie Beer
Summary: Recent research has focused on improving the use of apple pomace waste in the food and nutraceutical industries. This study quantified the polymeric procyanidins in apple pomace extracts for the first time and found that they were the most abundant polyphenol type. The presence of polymeric procyanidins in apple pomace extracts may overestimate their antioxidant activity in vivo.
Article
Public, Environmental & Occupational Health
Kim Moesgaard Iburg, Periklis Charalampous, Peter Allebeck, Elsa Jonsson Stenberg, Ronan O'Caoimh, Lorenzo Monasta, Jose L. Penalvo, David M. Pereira, Grant M. A. Wyper, Vikram Niranjan, Brecht Devleesschauwer, Juanita Haagsma
Summary: This study examines the development of disease burden among older adults in Europe over the last 30 years. The results indicate an overall increase in disease burden among older Europeans, especially in Western Europe. Eastern Europe experienced a decrease in DALYs between 2006 and 2013. There are significant differences in DALY rates among regions and genders, with cardiovascular diseases being the major contributor to changes in YLL rates.
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Silvia Montoro-Garcia, Sara Martinez-Sanchez, Miguel Carmena-Bargueno, Horacio Perez-Sanchez, Maria Campillo, Camille Oger, Jean-Marie Galano, Thierry Durand, Angel Gil-Izquierdo, Jose Antonio Gabaldon
Summary: The effects of 9-D1t-PhytoP on platelet activation and vascular cells were tested, along with possible interactions with platelets and the endothelial EP3 receptor. The results showed that 9-D1t-PhytoP has prothrombotic and wound-healing properties, and mimics the migration stimulus of PGE(2) in endothelial cells. Computational analysis revealed its potential as an EP3 agonist by forming a stable complex with the EP3 receptor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lucia Bada, Renato B. Pereira, David M. Pereira, Marta Lores, Maria Celeiro, Elias Quezada, Eugenio Uriarte, Jose Gil-Longo, Dolores Vina
Summary: The genus Ulex consists of thirteen accepted species of perennial shrubs in the Fabaceae family. In Galicia, Spain, many of these species are considered spontaneous colonizers that are easy to establish and maintain. Among them, Ulex gallii Planch. is used in traditional medicine for its anti-infective, hypotensive, and diuretic properties. Limited scientific studies have been conducted on Ulex gallii Planch., and its composition has not been reported. In this study, different metabolites, mainly flavonoids, were tentatively identified in the sub-fractions of Ulex gallii Planch., which exhibited antiproliferative activity against lung and stomach cancer cell lines.
Article
Oncology
Daniela Correia da Silva, Patricia Valentao, David M. Pereira
Summary: Cancer is the second leading cause of death globally, and finding effective treatments is urgent. This study aimed to identify selective cytotoxic natural products that target the endoplasmic reticulum (ER) of cancer cells. The ER plays a key role in cellular protein synthesis and folding. ER stress is a known vulnerability of cancer cells due to their abnormal protein synthesis rates. Several natural products were found to induce selective cytotoxicity in cancer cells by acting on the ER. Berberine was the most promising molecule, disrupting Ca2+ homeostasis and activating the unfolded protein response (UPR) and ER-resident caspase-4 in cancer cells. These findings suggest that berberine and emodin have potential for the development of new cancer treatments.
Article
Biology
Vera Ribeiro, Federico Ferreres, Andreia Oliveira, Nelson G. M. Gomes, Angel Gil-Izquierdo, Luisa Araujo, David Pereira, Patricia Valentao
Summary: This study aimed to investigate the mechanisms and bioactives involved in the cytotoxicity of Allophylus africanus stem bark extract in human stomach cancer cells. The extract exhibited selective cytotoxicity against AGS cells and induced apoptosis. It was found that the cytotoxic effects were attributed to the presence of vicenin-2 and other apigenin derivatives. These findings suggest the potential of A. africanus stem bark as an anticancer agent against gastric adenocarcinoma and highlight the importance of exploring herbal-based products and apigenin derivatives in chemotherapy drug development.
Article
Food Science & Technology
Catarina Andrade, David M. Pereira, Nelson G. M. Gomes, Federico Ferreres, Angel Gil-Izquierdo, Paula B. Andrade, Sutsawat Duangsrisai, Patricia Valenta
Summary: Kitul (Caryota urens L.) inflorescences are widely used for sweet sap production in Asian countries and have potential antidiabetic properties. In this study, the methanol extract of the inflorescences was shown to reduce reactive species in pancreatic cells and inflammatory markers in stimulated macrophages. The extract also inhibited 5-lipoxygenase activity. These findings suggest that Kitul inflorescences may have pleiotropic mechanisms as an antidiabetic agent.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Mari Merce Cascant-Vilaplana, Eduardo Viteritti, Victor Sadras, Sonia Medina, Maria Puerto Sanchez-Iglesias, Camille Oger, Jean-Marie Galano, Thierry Durand, Jose Antonio Gabaldon, Julian Taylor, Federico Ferreres, Manuel Sergi, Angel Gil-Izquierdo
Summary: This study investigates the impact of aphids feeding on wheat plants and their role in triggering a defensive plant reaction to oxidative stress. The experiment involves different nitrogen and CO2 concentrations, as well as two types of aphids. The results show that aphids significantly affect the levels of certain oxylipins in wheat leaves, indicating their ability to manipulate plant defense mechanisms.
Article
Biochemistry & Molecular Biology
Raquel Martins, Fatima Fernandes, Patricia Valentao
Summary: This study reveals the phenolic profile of the aqueous extract of Solanum betaceum leaves for the first time, and demonstrates its potential as an antidiabetic agent and antioxidant.
Article
Agronomy
Maria Pinciroli, Raul Dominguez-Perles, Sonia Medina, Camille Oger, Alexandre Guy, Thierry Durand, Mari Merce Cascant-Vilaplana, Jose Antonio Gabaldon-Hernandez, Federico Ferreres, Angel Gil-Izquierdo
Summary: This study aimed to investigate the effects of exogenously foliar-applied salicylic acid (SA) on oxidative stress in rice. The concentrations of PhytoPs and PhytoFs in immature and mature grains of three different genotypes of rice were monitored, and their influence on grains per panicle and chalkiness was examined. The results showed that SA reduced oxylipin levels and increased rice yield.
Article
Biochemistry & Molecular Biology
Raul Arcusa, Juan Angel Carillo, Begona Cerda, Thierry Durand, Angel Gil-Izquierdo, Sonia Medina, Jean-Marie Galano, Maria Pilar Zafrilla, Javier Marhuenda
Summary: This study aimed to evaluate the efficacy of long-term consumption of high polyphenolic content from fruits and vegetables (such as berries) as a nutraceutical against central nervous system lipid peroxidation, as assessed through urine biomarkers. The results showed significant reductions in AdA and DHA-derived metabolites after consumption of the nutraceutical, indicating its effectiveness in preventing CNS lipid peroxidation.
Article
Infectious Diseases
Rute Moreira, Federico Ferreres, Angel Gil-Izquierdo, Nelson G. M. Gomes, Luisa Araujo, Eugenia Pinto, Paula B. Andrade, Romeu A. Videira
Summary: Although the crude methanol extract (CME) and the methanol fraction (MF) of Guiera senegalensis leaves exhibited poor antifungal activity, the ethyl acetate fraction (EAF) demonstrated strong antifungal activity against various fungi, as well as potent effectiveness against certain yeasts.
Article
Biochemical Research Methods
Daniela Mendes, Ana Maria Silva, Maria Manuel Oliveira, Paula B. B. Andrade, Romeu A. A. Videira
Summary: Mitochondrial dysfunction and cytosolic oxidative stress are interconnected pathological biomarkers in chronic diseases and cellular toxicity induced by high-energy radiation or xenobiotics. Thus, evaluating the activities of mitochondrial redox chain complexes and cytosolic antioxidant enzymes in the same cell culture system is a valuable approach for studying chronic diseases and understanding the molecular mechanisms underlying physical and chemical stress agents' toxicity. This article provides experimental procedures to obtain mitochondria-free cytosolic and mitochondria-rich fractions from isolated cells, as well as methodologies to assess the activities of antioxidant enzymes and mitochondrial complexes. The protocols were optimized to enable testing with a single T-25 flask of 2D cultured cells, as demonstrated in the typical results discussed.
METHODS AND PROTOCOLS
(2023)