Article
Chemistry, Medicinal
Katarina Cerne, Nusa Kelhar, Natasa Resnik, Marusa Herzog, Lana Vodnik, Peter Veranic, Borut Kobal
Summary: Platinum-resistant high-grade serous ovarian cancer (HGSOC) is a fatal disease and new strategies to overcome platinum resistance are needed. Extracellular vesicles (EVs), especially EpCAM-specific EVs, show promise as potential biomarkers for predicting chemoresistance. EVs released from chemoresistant HGSOC cell lines demonstrated greater size heterogeneity, a higher proportion of medium/large EVs, and a higher number of released EpCAM-positive EVs. There was also a strong positive correlation between the concentration of EpCAM-positive EVs and the expression of cellular EpCAM. These findings may contribute to predicting platinum resistance in the future, but further validation in clinical samples is necessary.
Article
Gastroenterology & Hepatology
Rocio Munoz-Hernandez, Sheila Gato, Antonio Gil-Gomez, Rocio Aller, Angela Rojas, Laura Moran, Javier Gallego, Elena Blazquez-Lopez, Rocio Gallego-Duran, Rocio Montero-Vallejo, Vanessa Garcia-Fernandez, Douglas Maya-Miles, Maria del C. Rico, Francisco J. Cubero, Javier Vaquero, Javier Ampuero, Rafael Banares, Manuel Romero-Gomez
Summary: This study evaluated the circulating AV+ EpCAM+ CD133+ EVs as a potential biomarker for the transition from simple steatosis to steatohepatitis. The results showed that these EVs increased during disease progression in both mouse models and clinical validation. Therefore, EpCAM+ CD133+ EVs hold promise as a non-invasive biomarker for evaluating and managing NAFLD patients.
LIVER INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Xingle Yu, Xiaohui Chen, Zixin Sun, Ruyan Niu, Yun Deng, Liu Wang, Ying Zhu, Liangliang Zhang, Hong Zhang, Kang Wang, Jichun Yang, Wei Gu, Guoxiang Liu, Yang Luo
Summary: This study proposed a DNAzyme-triggered assembly and disassembly system for the conversion of single nano-sized sEVs into clusters, which could be enriched by centrifugation and then broken into single sEVs in the presence of magnesium ions. The quantification of sEVs was achieved by recording the increase in fluorescence upon nucleic acid cleavage, with a detection limit as low as 54 particles/μL. The entire process could be completed in 1.5 hours without the need for ultracentrifugation.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Khrystany T. Isebia, Eshwari Dathathri, Noortje Verschoor, Afroditi Nanou, Anouk C. De Jong, Frank A. W. Coumans, Leon W. M. M. Terstappen, Jaco Kraan, John W. M. Martens, Ruchi Bansal, Martijn P. Lolkema
Summary: This study compared the composition of circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (tdEVs) in the blood of patients with metastatic castration-naive prostate cancer (CNPC) and metastatic castration-resistant prostate cancer (CRPC). The study found potential differences between these biomarkers in the two disease stages, with lower CTC and tdEV levels in CNPC compared to CRPC patients. Furthermore, the study observed a decline in CTC/tdEV numbers 6 months after androgen deprivation therapy (ADT).
Article
Chemistry, Analytical
Tianlong Zhang, David W. Inglis, Long Ngo, Yuling Wang, Yoichiroh Hosokawa, Yaxiaer Yalikun, Ming Li
Summary: This work proposes a novel method for continuous separation of particles by size using inertial microfluidics, with the assistance of symmetrical sheath flows in a straight microchannel. Experimental data show that the separation performance of the developed microfluidic device is affected by three main factors: channel length, total flow rate, and flow rate ratio of sheath to sample. Besides, the separation of platelets from whole blood is demonstrated. This systematic study provides a new perspective for particle separation, which is expected to find applications across various fields spanning physics, biology, biomedicine, and industry.
ANALYTICAL CHEMISTRY
(2023)
Article
Biology
Abel Sana, Izadora Volpato Rossi, Bruna Sabatke, Leticia Bassani Bonato, Lia Carolina Soares Medeiros, Marcel Ivan Ramirez
Summary: This study aimed to enrich the large extracellular vesicles (LEVs) of G. intestinalis and better understand their role in the parasite's interaction with the host. The researchers modified their method and compared different centrifugation techniques to concentrate the EVs. They found that direct ultracentrifugation and longer centrifugation time were more effective in enriching the EVs.
Article
Chemistry, Analytical
Xiaohui Chen, Yun Deng, Ruyan Niu, Zixin Sun, Alya Batool, Liu Wang, Chong Zhang, Ningyu Ma, Qingtang Yang, Guoxiang Liu, Jichun Yang, Yang Luo
Summary: In this study, a Programmable Isothermal Cascade Keen Enzyme-free Reporter (PICKER) was constructed for the accurate detection of cancer-derived small extracellular vesicles (csEVs). A novel particle ratio index (PRcsEV/tsEV) was proposed as a potential indicator of breast cancer staging.
ANALYTICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Kecheng Lou, Shangzhi Feng, Hui Luo, Junrong Zou, Guoxi Zhang, Xiaofeng Zou
Summary: Macrophages and their extracellular vesicles play significant roles in tumor microenvironment and cancer development, and show potential for clinical applications in immunomodulation, cancer therapy, infectious defense, and tissue repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Oncology
Yiyang Liu, Yufei Wang, Sheng Sun, Zeyu Chen, Shuai Xiang, Zeyang Ding, Zhao Huang, Bixiang Zhang
Summary: This article investigates the role of EpCAM in cancer and its communication with other signaling pathways. It introduces the impact on the biology of cancers and the latest clinical applications of EpCAM, aiming to shed light on its translational prospects.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2022)
Article
Chemistry, Analytical
Xiaowei Wei, Lijun Cai, Hanxu Chen, Luoran Shang, Yuanjin Zhao, Weijian Sun
Summary: This study proposes a novel system for multiplexed analysis of bladder cancer-derived urine exosomes using magnetic microspheres as barcoded microcarriers. The unique structure of the microcarriers allows for controllable movement and high sensitivity detection of exosomes. The noninvasive nature of the platform, combined with its good sensitivity, specificity, low sample consumption, and ease of use, indicates its great potential for bladder cancer diagnosis in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yanmei Lei, Xiaochen Fei, Yue Ding, Jianhui Zhang, Guihua Zhang, Liang Dong, Jia Song, Ying Zhuo, Wei Xue, Peng Zhang, Chaoyong Yang
Summary: The study developed a precise assay called SORTER to detect tumor-derived exosomal miRNAs and enhance the diagnostic accuracy of prostate cancer (PCa). The SORTER achieved selective sorting of tumor-specific exosome subtypes and enabled in situ profiling of tumor-derived exosomal miRNAs. With a signature of six miRNAs, SORTER differentiated PCa and benign prostatic hyperplasia with high accuracy, and showed significant accuracy in classifying metastatic and nonmetastatic PCa.
Article
Chemistry, Analytical
Xiaohui Chen, Yun Deng, Ruyan Niu, Zixin Sun, Alya Batool, Liu Wang, Chong Zhang, Ningyu Ma, Qingtang Yang, Guoxiang Liu, Jichun Yang, Yang Luo
Summary: In this study, a Programmable Isothermal Cascade Keen Enzyme-free Reporter (PICKER) was constructed to accurately detect and acquire the relative abundance of cancer-derived small extracellular vesicles (csEVs) in total small extracellular vesicles (tsEVs). By integrating dual-aptamer recognition and catalytic hairpin assembly amplification, the PICKER showed a detection limit of 420 particles/μL csEVs. A novel particle ratio index of csEV against tsEV (PRcsEV/tsEV) was proposed to eliminate errors from inconsistent centrifugation. PRcsEV/tsEV value was found to increase proportionally to breast cancer stages.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Rafal Szatanek, Monika Baj-Krzyworzeka
Summary: Cancer metastasis involves complex interactions between various cell types and the tumor microenvironment, with tumor cells releasing proteins and nucleic acids through extracellular vesicles to facilitate dissemination. This process plays a crucial role in tumor progression and escape from immune surveillance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Su Jin Kang, Si Eun Kim, Myung-Ji Seo, Eunjung Kim, Won Jong Rhee
Summary: The study found that onion-derived extracellular vesicles (Onex) have potential anti-inflammatory activity, suppressing the secretion and expression of inflammatory factors and showing promise in treating diseases accompanied by severe inflammation.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Review
Cell Biology
Su-Ran Li, Qi-Wen Man, Xin Gao, Hao Lin, Jing Wang, Fu-Chuan Su, Han-Qi Wang, Lin-Lin Bu, Bing Liu, Gang Chen
Summary: Extracellular vesicles (EVs) are lipid-bilayer membrane structures secreted by most cell types, acting as messengers through the transfer of lipids, proteins, and nucleic acids to influence cellular processes. Tissue-derived EVs (Ti-EVs) offer advantages such as tissue specificity and accurate reflection of the tissue microenvironment, playing important roles in intercellular communication and disease development insights. Current research on Ti-EVs is limited compared to body fluid or cell culture-derived EVs, highlighting the need for further exploration in basic and clinical applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Cell Biology
Linda G. Rikkert, Frank A. W. Coumans, Chi M. Hau, Leon W. M. M. Terstappen, Rienk Nieuwland
Summary: A study developed a fast and simple single-step centrifugation protocol for producing high-quality platelet-free plasma for EV research. Compared to the traditional two-step protocol, the single-step protocol is faster, removes platelets effectively, and has a slightly higher plasma yield.
Article
Oncology
Pauline A. J. Mendelaar, Jaco Kraan, Mai Van, Leonie L. Zeune, Leon W. M. M. Terstappen, Esther Oomen-de Hoop, John W. M. Martens, Stefan Sleijfer
Summary: The study analyzed the size characteristics of CTCs in different cancer patients and found significant size differences among different tumor types, with CTCs being significantly smaller than cell line tumor cells. This has implications for designing and optimizing size-based isolation methods for CTCs.
MOLECULAR ONCOLOGY
(2021)
Article
Genetics & Heredity
Lars C. Steggink, Hink Boer, Coby Meijer, Joop D. Lefrandt, Leon W. M. M. Terstappen, Rudolf S. N. Fehrmann, Jourik A. Gietema
Summary: Genetic variation may play a role in the increased risk of cardiovascular disease in chemotherapy-treated testicular cancer patients. The study identified 141 independent genomic loci associated with CVD occurrence in this patient population, and DEPICT highlighted ten biological themes related to CVD.
PHARMACOGENOMICS JOURNAL
(2021)
Article
Medicine, General & Internal
Michiel Stevens, Peng Liu, Tom Niessink, Anouk Mentink, Leon Abelmann, Leon Terstappen
Summary: This study optimized the immunomagnetic enrichment process using a flow-through magnetophoretic system to increase sample size for more accurate treatment effects measurement and personalized therapy guidance. Experimental data confirmed the effectiveness of optimizing magnetophoretic separation methods in combination with cell lines of varying EpCAM antigen expression.
Correction
Biology
Pepijn Beekman, Agustin Enciso-Martinez, Sidharam P. Pujari, Leon W. M. M. Terstappen, Han Zuilhof, Severine Le Gac, Cees Otto
COMMUNICATIONS BIOLOGY
(2021)
Article
Biology
Pepijn Beekman, Agustin Enciso-Martinez, Sidharam P. Pujari, Leon W. M. M. Terstappen, Han Zuilhof, Severine Le Gac, Cees Otto
Summary: The study found that organosilicon compounds may interact with cell membranes passively and be adsorbed in or on the cell membrane; these compounds are commonly present in everyday items and widely used in food, cosmetics, and pharmaceuticals; with the increasing application of organosilicon compounds as replacements for other plastics, there is a need for a deeper understanding of these interactions.
COMMUNICATIONS BIOLOGY
(2021)
Correction
Biology
Pepijn Beekman, Agustin Enciso-Martinez, Sidharam P. Pujari, Leon W. M. M. Terstappen, Han Zuilhof, Severine Le Gac, Cees Otto
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Menno Tamminga, Kiki C. Andree, Hilda van den Bos, T. Jeroen N. Hiltermann, Anouk Mentink, Diana C. J. Spierings, Peter Lansdorp, Wim Timens, Ed Schuuring, Leon W. M. M. Terstappen, Harry J. M. Groen
Summary: DLA detected nine times more CTCs than in the peripheral blood, and the sustained presence of CTCs in DLA after treatment was associated with therapy failure and shortened PFS.
BRITISH JOURNAL OF CANCER
(2022)
Article
Oncology
Fikri Abali, Narges Baghi, Lisanne Mout, Joska J. Broekmaat, Arjan G. J. Tibbe, Leon W. M. M. Terstappen
Summary: The study highlights the importance of measuring protein secretion from individual cancer cells and how it can be influenced by different drugs. It shows that this method has the potential to accelerate drug development, enhance understanding of therapy resistance, and improve personalized cancer treatment.
Article
Oncology
Michiel Stevens, Afroditi Nanou, Leon W. M. M. Terstappen, Christiane Driemel, Nikolas H. Stoecklein, Frank A. W. Coumans
Summary: This article explores the application of a deep learning-based segmentation method called StarDist in the enumeration of circulating tumor cells (CTC). Compared to the CellSearch system, StarDist can accurately segment CTC, especially in samples with high cell density.
Article
Oncology
Khrystany T. Isebia, Eshwari Dathathri, Noortje Verschoor, Afroditi Nanou, Anouk C. De Jong, Frank A. W. Coumans, Leon W. M. M. Terstappen, Jaco Kraan, John W. M. Martens, Ruchi Bansal, Martijn P. Lolkema
Summary: This study compared the composition of circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (tdEVs) in the blood of patients with metastatic castration-naive prostate cancer (CNPC) and metastatic castration-resistant prostate cancer (CRPC). The study found potential differences between these biomarkers in the two disease stages, with lower CTC and tdEV levels in CNPC compared to CRPC patients. Furthermore, the study observed a decline in CTC/tdEV numbers 6 months after androgen deprivation therapy (ADT).
Article
Oncology
Tao Lu, Lisa Oomens, Leon W. M. M. Terstappen, Jai Prakash
Summary: Cancer-associated fibroblasts (CAFs) are important drivers in the tumor microenvironment and facilitate the growth and survival of tumor cells, as well as metastasis formation. In this study, we aimed to study circulating CAFs (cCAFs) and circulating tumor cells (CTCs) in a preclinical breast tumor model in mice in order to understand the effect of chemotherapy on cCAFs and CTC formation. We found that tumors with CAFs grew faster than tumors without CAFs. Furthermore, chemotherapy may exacerbate the formation of cCAFs and CTCs, which may eventually support the formation of a metastasis niche in breast cancer.
Meeting Abstract
Oncology
Christina Guo, Mateus Crespo, Bora Gurel, David Dolling, Jan Rekowski, Adam Sharp, Antonella Petremolo, Semini Sumanasuriya, Daniel N. Rodrigues, Ana Ferreira, Rita Pereira, Ines Figueiredo, Niven Mehra, Maryou B. K. Lambros, Antje Neeb, Veronica Gil, Leon Terstappen, Andrea Alimonti, Charles G. Drake, Wei Yuan, Johann S. de Bono
CANCER IMMUNOLOGY RESEARCH
(2021)
