Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Article
Multidisciplinary Sciences
Peter R. Corridon
Summary: This study examines the microarchitecture of acellular kidney scaffolds using intravital microscopy (IVM) data. The effectiveness of decellularization and the performance of acellular nephron compartments after transplantation were evaluated. The results contribute to the broader understanding and application of this imaging technique in analyzing scaffold systems.
Article
Cardiac & Cardiovascular Systems
Adam Nyul-Toth, Stefano Tarantini, Jordan DelFavero, Feng Yan, Priya Balasubramanian, Andriy Yabluchanskiy, Chetan Ahire, Tamas Kiss, Tamas Csipo, Agnes Lipecz, Attila E. Farkas, Imola Wilhelm, Istvan A. Krizbai, Qinggong Tang, Anna Csiszar, Zoltan Ungvari
Summary: Age-related blood-brain barrier disruption and cerebromicrovascular rarefaction play important roles in the pathogenesis of vascular cognitive impairment and dementia (VCID) and Alzheimer's disease (AD). Recent advances in geroscience research have provided new interventions for preventing these diseases. Sensitive and easy-to-adapt imaging methods are urgently needed for longitudinal assessment of changes in BBB permeability and brain capillarization in aged mice.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Markus Seeger, Antonios Stylogiannis, Ludwig Prade, Sarah Glasl, Vasilis Ntziachristos
Summary: By using overdriving continuous-wave laser diodes and frequency-wavelength multiplexing based on illumination pulse-trains, we demonstrate concurrent multi-wavelength optoacoustic microscopy with high signal-to-noise ratios and high resolution, allowing visualization of vascular oxygenation dynamics and circulating tumor cells.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Yingxian Zhang, Jincheng Zhong, Hui Cheng, Jie Huang, Zhenhui Li, Chi Zhang, Zhiang Gao, Zhourui Xu, Gaixia Xu, Ping Qiu, Ke Wang
Summary: By detailed measurements and comparison of the effective attenuation length (l(e)) in different excitation wavelengths, this study demonstrates that 3-photon microscopy at 1700 nm enables the deepest penetration in brain imaging in vivo.
NANOSCALE ADVANCES
(2023)
Review
Urology & Nephrology
Joana R. Martins, Dominik Haenni, Milica Bugarski, Marcello Polesel, Claus Schuh, Andrew M. Hall
Summary: The development of intravital imaging with multiphoton microscopy has revolutionized kidney research by allowing real-time visualization of dynamic cellular behavior and organelle function, providing insights into structure-function relationships in unprecedented detail. Advances in technology and image analysis have overcome practical challenges, opening up huge new possibilities for studying cellular mechanisms and disease states in the kidney.
KIDNEY INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Michael Hamersky, Khushi Tekale, L. Matthew Winfree, Mathew J. M. Rowan, Lindsey Seldin
Summary: Understanding cell behaviors is crucial for disease intervention, and optimizing imaging methods is necessary for observing cell dynamics. This study presents a customized microscope stage insert for inverted confocal microscopes, facilitating intravital imaging in intact animals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Multidisciplinary Sciences
Juo-Nang Liao, Wei-Liang Chen, Chao-Yuan Lo, Man-Hong Lai, Huang-Lung Tsai, Yu-Ming Chang
Summary: In this study, a nondestructive method using two-photon fluorescence and second-harmonic generation imaging was demonstrated to quantify starch granules within chloroplasts of intact leaves across a day-night cycle. The results provided micron scale spatial resolution of starch distribution within leaves and showed starch circadian patterns consistent with previous studies. This method is a potential tool for revealing the real-time heterogeneity of starch circadian rhythm in leaf cells without the need for destructive sample preparation.
SCIENTIFIC REPORTS
(2022)
Article
Optics
Kong Gao, Yizhou Liu, Wenchao Qiao, Yuzhi Song, Xian Zhao, Aimin Wang, Tao Li
Summary: In this study, an ytterbium-doped stretched-pulse mode-locked fiber oscillator was fabricated using a nonlinear amplifier loop mirror (NALM). The central wavelength of the laser can be modified by tuning the intra-cavity loss. The laser achieved high output power and short compressed pulse durations.
Article
Biochemistry & Molecular Biology
Sammy Hermans, Jacob Pilon, Dennis Eschweiler, Johannes Stegmaier, Carmen A. H. Severens-Rijvers, Salwan Al-Nasiry, Marc van Zandvoort, Dimitrios Kapsokalyvas
Summary: Pre-eclampsia is a severe complication in pregnancy with limited diagnostic and therapeutic options, and there is controversy surrounding its etiology and phenotypes. Using multiphoton microscopy (MPM) imaging, the native placental three-dimensional morphology can be visualized with subcellular resolution, allowing for the identification of various imaging targets such as trophoblast organization, villous tree structure, syncytial knots, fibrosis, and vascular networks. Preliminary data suggests that pre-eclampsia is characterized by increased syncytial knot density, paddle-like villous sprouts, abnormal villous volume-to-surface ratio, and decreased vascular density. Quantifying 3D microscopic images shows potential for phenotyping pre-eclampsia in placental villous tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
A. A. Gopal, A. Kazarine, J. M. Dubach, P. W. Wiseman
Summary: Nonlinear microscopy utilizes nonlinear interactions between light and matter to image fluorescence and scattering in biological tissues, requiring high peak intensities and spatial confinement for deep imaging. This technique offers various contrast mechanisms and instrumentation for different purposes.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Defu Chen, David W. Nauen, Hyeon-Cheol Park, Dawei Li, Wu Yuan, Ang Li, Honghua Guan, Carmen Kut, Kaisorn L. Chaichana, Chetan Bettegowda, Alfredo Quinones-Hinojosa, Xingde Li
Summary: This study systematically investigated the feasibility of using multiphoton microscopy for rapid histopathological assessment of label-and processing-free surgical specimens, focusing on infiltrating glioma. The results showed that multiphoton microscopy robustly captures diagnostic features of glioma and deep learning can assist with image recognition.
Article
Archaeology
M. Martinez-Weinbaum, L. Maestro-Guijarro, P. M. Carmona-Quiroga, S. Siano, D. Ciofini, M. Castillejo, M. Oujja
Summary: This study investigates the extent of degradation of varnish coatings at different depths due to aging. By determining factors such as varnish type, aging time, and initial thickness, we can assess the degree of varnish deterioration non-invasively.
JOURNAL OF CULTURAL HERITAGE
(2023)
Review
Immunology
Meirong Li, Qian Hou, Lingzhi Zhong, Yali Zhao, Xiaobing Fu
Summary: The review highlights the persistent hyper-inflammation as a distinguishing characteristic of chronic wounds, with macrophage malfunction identified as a major contributor. The study explores the origin, heterogeneity, and dysregulation of macrophages in wound healing, while also evaluating therapeutic approaches for restoring macrophage response. Multidimensional analysis using high-throughput technologies helps assess the diversity, activation states, and communication of macrophages in healing and non-healing wounds, providing promising interventions for chronic wounds.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Despoina Kantere, Noora Neittaanmaki, Kristina Maltese, Ann-Marie Wennberg Larko, Petra Tunback
Summary: This study investigated the potential of reflectance confocal microscopy (RCM) for diagnosing genital lichen sclerosus (LS). Prominent fiber-like structures observed in RCM images were able to accurately diagnose LS. RCM can be a valuable tool for monitoring LS patients for abnormal growth, providing a non-invasive diagnostic tool for genital disorders.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Dermatology
Jake D. Jones, Marcos R. Rodriguez, Kyle P. Quinn
Summary: In this study, convolutional neural networks (CNNs) were successfully trained and applied to automatically segment skin wounds imaged by multiphoton microscopy (MPM), enabling automated and rapid calculation of wound geometry and metabolism. The CNNs achieved high accuracy on different sets of samples, providing a broader prospect for the application of MPM technology.
LASERS IN SURGERY AND MEDICINE
(2021)
Letter
Dermatology
Jake D. Jones, Kyle P. Quinn
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Biology
Jake D. Jones, Hallie E. Ramser, Alan E. Woessner, Kyle P. Quinn
COMMUNICATIONS BIOLOGY
(2018)