Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1857, Issue 4, Pages 454-461Publisher
ELSEVIER
DOI: 10.1016/j.bbabio.2016.01.014
Keywords
Hydrogenase; Nitric oxide; Inhibition mechanism; EPR spectroscopy; Protein film voltammetry; Metalloenzyme
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Funding
- CNRS
- Aix-Marseille Universite
- Agence Nationale de la Recherche (ANR HEROS) [ANR-14-CE05-0010-01]
- A*Midex foundation of Aix-Marseille University (project MicrobioE) [ANR-11-IDEX-0001-02]
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Hydrogenases reversibly catalyze the oxidation of molecular hydrogen and are inhibited by several small molecules including O-2, CO and NO. In the present work, we investigate the mechanism of inhibition by NO of the oxygen-sensitive NiFe hydrogenase from Desulfovibrio fructosovorans by coupling site-directed mutagenesis, protein film voltammetry (PFV) and EPR spectroscopy. We show that micromolar NO strongly inhibits NiFe hydrogenase and that the mechanism of inhibition is complex, with NO targeting several metallic sites in the protein. NO reacts readily at the NiFe active site according to a two-step mechanism. The first and faster step is the reversible binding of NO to the active site followed by a slower and irreversible transformation at the active site. NO also induces irreversible damage of the iron-sulfur centers chain. We give direct evidence of preferential nitrosylation of the medial [3Fe-4S] to form dinitrosyl-iron complexes. (C) 2016 Elsevier B.V. All rights reserved.
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