Journal
BIOCHEMISTRY-MOSCOW
Volume 81, Issue 13, Pages 1754-1770Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297916130113
Keywords
immuno-PCR; quantitative PCR; antibody; antigen; DNA-antibody conjugate
Categories
Funding
- Russian Science Foundation [16-14-00136]
- RF [SP-4413.2015.4]
- Russian Science Foundation [16-14-00136] Funding Source: Russian Science Foundation
Ask authors/readers for more resources
The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of visualization of antigen-antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available