Journal
BIOPHYSICAL JOURNAL
Volume 109, Issue 1, Pages 3-6Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2015.05.026
Keywords
-
Categories
Funding
- European Union Marie Curie Action [FP7-PEOPLE-2013-IEF 630024]
- Sinergia grant from the Swiss National Science Foundation [CRS113-141945]
Ask authors/readers for more resources
Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical challenges. In this Letter we provide a straightforward approach to single-molecule localization microscopy imaging in three dimensions and two colors. We combine biplane imaging and spectral-demixing, which eliminates a number of problems, including color cross-talk, chromatic aberration effects, and problems with color registration. We present 3D dual-color images of nanoscopic structures in hippocampal neurons with a 3D compound resolution routinely achieved only in a single color.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available