Article
Multidisciplinary Sciences
Rawad Hodeify, Mohamed Chakkour, Reem Rida, Sawsan Kreydiyyeh
Summary: The Na+/K+ ATPase plays a crucial role in regulating the ionic homeostasis of hepatocytes, and its stimulation by PGE2 is mediated through EP4, PKA, and intracellular calcium pathways.
Article
Cell Biology
Niraj Nepal, Subha Arthur, Jennifer Haynes, Balasubramanian Palaniappan, Uma Sundaram
Summary: PGE2 inhibits the activity of Na-K-ATPase in villus cells by activating the PKA pathway, leading to a transcriptional reduction in the alpha 1 and beta 1 subunit genes of Na-K-ATPase.
Article
Cell Biology
Susana Goncalves, Kelvin Yin, Yoko Ito, Adelyne Chan, Ioana Olan, Sarah Gough, Liam Cassidy, Eva Serrao, Stephen Smith, Andrew Young, Masashi Narita, Matthew Hoare
Summary: COX2 plays a critical role in regulating SASP composition and senescence surveillance during RAS-induced senescence. Its downstream product, prostaglandin E-2 (PGE(2)), is also implicated in tumor suppression and shaping the immune microenvironment during senescence. Loss of COX2 dysregulates immune cells and impairs senescence surveillance in early tumorigenesis.
Article
Multidisciplinary Sciences
Jue Zhang, Jackie Chang, Mirza Ahmar Beg, Wenxin Huang, Yiqiong Zhao, Wen Dai, Xiaopeng Wu, Weiguo Cui, Sneha S. Pillai, Hari Vishal Lakhani, Komal Sodhi, Joseph I. Shapiro, Daisy Sahoo, Ze Zheng, Roy L. Silverstein, Yiliang Chen
Summary: This study found that NKA alpha 1 plays a role in suppressing LPS-induced pro-inflammatory signaling through the inhibition of Lyn.
Article
Biochemistry & Molecular Biology
Xin Yang, Maodi Liang, Yihan Tang, Dingling Ma, Menghuan Li, Chenggang Yuan, Yanting Hou, Chaoyue Sun, Jie Liu, Qianqian Wei, Yongsheng Chang, Cuizhe Wang, Jun Zhang
Summary: In the context of obesity, inflammatory cytokines secreted by adipocytes contribute to insulin resistance and play a key role in the development of metabolic syndrome. Our previous study found that the transcription factor KLF7 promotes the expression of p-p65 and IL-6 in adipocytes, but the specific molecular mechanism remains unclear. In this study, we found that the expression of KLF7, PKC?, p-I?B, p-p65, and IL-6 was significantly increased in epididymal white adipose tissue (Epi WAT) of mice fed a high-fat diet (HFD). However, in KLF7 fat conditional knockout mice, the expression of PKC?, p-I?B, p-p65, and IL-6 was significantly decreased in Epi WAT. In 3T3-L1 adipocytes, KLF7 promotes the expression of IL-6 through the PKC?/NF-?B pathway. Furthermore, luciferase reporter and chromatin immunoprecipitation assays confirmed that KLF7 upregulates the expression of PKC? transcripts in HEK-293T cells. Together, our results demonstrate that KLF7 promotes the expression of IL-6 by upregulating PKC? expression and activating the NF-?B signaling pathway in adipocytes.
Article
Agriculture, Multidisciplinary
Fang Wang, Yuanrong Chen, Kiyoshi Itagaki, Bin Zhu, Yajuan Lin, Haizhao Song, Luanfeng Wang, Ling Xiong, Zebin Weng, Xinchun Shen
Summary: Wheat-germ-derived peptide APEPEPAF (APE) has protective properties against ulcerative colitis by regulating the PKC zeta/NF-kappa B signaling pathway, preserving colon barrier function, and improving intestinal flora imbalance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Francisca B. M. Sousa, Gabriella Pacheco, Ana P. Oliveira, Lucas A. D. Nicolau, Andre L. F. Lopes, Hygor Ferreira-Fernandes, Giovanny R. Pinto, Jand V. R. Medeiros
Summary: The study demonstrated that hydrogen sulfide (H2S) can protect against cholera toxin (CT) by preserving villus and crypt morphology in mice. This protective effect is likely achieved at least in part by reducing the activity of NF-kappa B and PGE2.
Article
Cell Biology
Seungjin Ryu, Jeehae Han, Trina M. Norden-Krichmar, Quanwei Zhang, Seunggeun Lee, Zhengdong Zhang, Gil Atzmon, Laura J. Niedernhofer, Paul D. Robbins, Nir Barzilai, Nicholas J. Schork, Yousin Suh
Summary: Gene variants associated with longevity were found to be enriched in the nPKC and NF-kappa B signaling pathways, with non-coding variants modulating the expression of cognate genes to reduce signaling. This matches genetic studies in other model organisms and suggests evolutionary conservation of reduced PKC and NF-kappa B signaling in exceptional longevity, possibly including humans.
Article
Biology
Junxiao Ren, Zhengzheng Hu, Quanlin Li, Shuang Gu, Fangren Lan, Xiqiong Wang, Jianbo Li, Junying Li, Liwa Shao, Ning Yang, Congjiao Sun
Summary: Chicken embryonic diapause is a state of temporary arrest of embryonic development triggered by cold stress-induced IRF1 expression, mediated by the PKC-NF-kappa B signaling pathway. This is in contrast to mammalian diapause which is mTOR-dependent. The study characterized the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in different diapause states and identified key regulators of diapause.
Article
Cell Biology
Dean Thumkeo, Siwakorn Punyawatthananukool, Somsak Prasongtanakij, Ryuma Matsuura, Kentaro Arima, Huan Nie, Rie Yamamoto, Naohiro Aoyama, Hisao Hamaguchi, Shingo Sugahara, Shinobu Takeda, Varodom Charoensawan, Atsushi Tanaka, Shimon Sakaguchi, Shuh Narumiya
Summary: In the tumor microenvironment, active inflammation and immunosuppression occur simultaneously and are regulated by the PGE(2)-EP2/EP4 signaling pathway. This study demonstrates that EP2/EP4 expression is strongly correlated with gene signatures of active inflammation and the mregDC-Treg axis, and has significant prognostic value in various human cancers.
Article
Biochemistry & Molecular Biology
Lei Xu, Yangfan Ye, Zeqiang Tao, Tian Wang, Yutian Wei, Wanzhi Cai, Xin Wan, Pengzhan Zhao, Wei Gu, Bin Gu, Liuchao Zhang, Yufei Tian, Ning Liu, Yiming Tu, Jing Ji
Summary: This study elucidated the role of MLPH in GBM radiation resistance, showing that MLPH promotes radiotherapy resistance by regulating the NF-kappa B pathway. Additionally, O-GlcNAcylation of MLPH protects it from degradation and stabilizes its presence in cells. These findings provide insights into a potential mechanism of GBM radiation resistance and suggest a therapeutic strategy for GBM treatment.
Article
Chemistry, Medicinal
Jingge Xu, Yan Wang, Zhen Wang, Lanping Guo, Xinpeng Li
Summary: The study investigated the effects of sulfated polysaccharides from Laminaria japonica on diabetic nephropathy symptoms, showing that the polysaccharides regulate diabetic nephropathy through the PKC/NF-kappa B pathway.
PHYTOTHERAPY RESEARCH
(2021)
Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Cell Biology
Nieves M. Gabrielli, Luciana C. Mazzocchi, Vitalii Kryvenko, Khodr Tello, Susanne Herold, Rory E. Morty, Friedrich Grimminger, Laura A. Dada, Werner Seeger, Jacob Sznajder, Istvan Vadasz
Summary: Alveolar hypoventilation leads to CO2 accumulation, impairing Na,K-ATPase beta subunit and disrupting cell-cell junctions, affecting alveolar integrity. Identification of the E3 ligase for Na,K-ATPase beta subunit may offer a novel therapeutic target for restoring epithelial function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Toxicology
Reem Rida, Rawad Hodeify, Sawsan Kreydiyyeh
Summary: FTY720P, a potential causative agent of inflammatory bowel disease, reduces the activity and membrane abundance of Na+/K+ ATPase through the activation of S1PR2, inhibition of ERK, activation of p38 MAPK, PKC, and PI3K, and release of PGE2.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Cell Biology
Ola El-Zein, Julnar Usta, Sawsan Ibrahim Kreydiyyeh
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2015)
Article
Food Science & Technology
Katherine Jaafar, Janay Haidar, Sawsan Kuraydiyyah, Tarek Ghaddar, Khouzama Knio, Baraem Ismail, Imad Toufeili
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2017)
Article
Cell Biology
Nadine Al Alam, Sawsan Ibrahim Kreydiyyeh
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2017)
Article
Multidisciplinary Sciences
Layla El Moussawi, Mohamed Chakkour, Sawsan I. Kreydiyyeh
Article
Biochemistry & Molecular Biology
Sawsan Ibrahim Kreydiyyeh, Zeina Dakroub
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2014)
Article
Plant Sciences
Hwaida Jaber, Elias Baydoun, Ola El-Zein, Sawsan Ibrahim Kreydiyyeh
PLANT FOODS FOR HUMAN NUTRITION
(2013)
Article
Multidisciplinary Sciences
Layla El Moussawi, Mohamed Chakkour, Sawsan Kreydiyyeh
Article
Fisheries
Reem Rida, Razan Zein-Eddine, Sawsan Kreydiyyeh, Antonio Garza de Yta, Imad Patrick Saoud
Summary: This study evaluated the effects of different salinities on the survival, growth, and osmoregulation in redclaw crayfish. The results showed that as salinity increased, the growth rate of redclaw crayfish decreased while sodium potassium ATPase activity and sodium potassium chloride cotransporter expression increased.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2021)
Article
Multidisciplinary Sciences
Rawad Hodeify, Mohamed Chakkour, Reem Rida, Sawsan Kreydiyyeh
Summary: The Na+/K+ ATPase plays a crucial role in regulating the ionic homeostasis of hepatocytes, and its stimulation by PGE2 is mediated through EP4, PKA, and intracellular calcium pathways.
Article
Toxicology
Reem Rida, Rawad Hodeify, Sawsan Kreydiyyeh
Summary: FTY720P, a potential causative agent of inflammatory bowel disease, reduces the activity and membrane abundance of Na+/K+ ATPase through the activation of S1PR2, inhibition of ERK, activation of p38 MAPK, PKC, and PI3K, and release of PGE2.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Food Science & Technology
Zena Wehbe, Sawsan Kreydiyyeh
Summary: The increase in bovine dairy consumption has been associated with the development of inflammatory diseases. Milk exosomes, which contain cytokines elevated in inflammatory bowel disease, are potential causative agents. This study investigates the relationship between exosomes and Na+/K+ ATPase using Caco-2 cells. The exosomes were found to dose and time-dependently increase ATPase activity, mediated by PGE2 and involving EP3 and EP4 receptors. The activation of ATPase was influenced by PKA and affected by EP3 and EP4 action.
Article
Cell Biology
Christine Khalil, Rawad Hodeify, Sawsan Kreydiyyeh
Summary: This study investigates the effect of S1P on renal Na+/K+ ATPase using FTY720P and LLC-PK1 cells. The results suggest that FTY720P activates S1PR2, Rho kinase, PI3K, leading to NO release and PKG stimulation, resulting in increased ATPase activity and protein expression.
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Review
Nutrition & Dietetics
Zena Wehbe, Sawsan Kreydiyyeh
Summary: Mammalian milk plays a crucial role in post-natal development, with microRNAs regulating over 60% of human genes. When humans consume cow's milk, bovine exosomes can be transported intact through the bloodstream and alter protein expression in target cells.
Article
Plant Sciences
Taha Deeb, Khouzama Knio, Zabta K. Shinwari, Sawsan Kreydiyyeh, Elias Baydoun
PAKISTAN JOURNAL OF BOTANY
(2013)