Article
Plant Sciences
Patrick Treffon, Jacopo Rossi, Giuseppe Gabellini, Paolo Trost, Mirko Zaffagnini, Elizabeth Vierling
Summary: Nitric oxide (NO) is a crucial signaling molecule in plants, regulated by the formation of S-nitrosoglutathione (GSNO) and the activity of the enzyme S-nitrosoglutathione reductase (GSNOR). Proteomic analysis of an Arabidopsis thaliana GSNOR null mutant revealed changes in proteins associated with chlorophyll metabolism and redox stress, as well as a significant increase in proteins from the aldo-keto reductase (AKR) protein superfamily. The study identified NADPH-dependent activity in GSNO reduction in plants, adding a new component to NO metabolism in plant cells.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Judong Kim, S. M. Touhidul Islam, Fei Qiao, Avtar K. Singh, Mushfiquddin Khan, Jeseong Won, Inderjit Singh
Summary: The research shows that regulating the cytokines released by B cells can protect against neuroinflammatory diseases like experimental autoimmune encephalomyelitis. Using GSNO and GSNOR inhibitor N6022 can modulate IL-10 and IL-6, offering potential in treating autoimmune disorders.
Article
Biochemistry & Molecular Biology
Eva Esther Rudolf, Patrick Huether, Ignasi Forne, Elisabeth Georgii, Yongtao Han, Ruediger Hell, Markus Wirtz, Axel Imhof, Claude Becker, Joerg Durner, Christian Lindermayr
Summary: The study reveals that GSNO reductase-deficient plants accumulate SAM, leading to increased methylation activity and significant changes in histone methylation status, which in turn affect chromatin accessibility and enhance DNA methylation.
Article
Biochemistry & Molecular Biology
Qianjin Liu, Tianle Gu, Ling-Yan Su, Lijin Jiao, Xinhua Qiao, Ting Xie, Lu-Xiu Yang, Dandan Yu, Ling Xu, Chang Chen, Yong-Gang Yao
Summary: GSNOR deficiency impairs antiviral innate immune response, promotes HSV-1 and VSV replication, leading to higher mortality and immune cell infiltration. GSNOR deficiency also enhances S-nitrosation of TBK1, inhibiting kinase activity and reducing interferon production.
Article
Biology
Rui Liu, Ting Zhu, Xin Chen, Zi Wang, Zhengyan Yang, Ang Ren, Liang Shi, Hanshou Yu, Mingwen Zhao
Summary: This study reveals that GSNOR regulates secondary metabolite production in microorganisms through S-nitrosylation of target proteins, specifically catalase. The findings shed light on the physiological functions of S-nitrosylated proteins and provide insights into the mechanism by which GSNOR responds to stress.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chunliu Pan, Xia Li, Shaochang Yao, Shuzhen Luo, Songying Liu, Aiqin Wang, Dong Xiao, Jie Zhan, Longfei He
Summary: Nitric oxide-mediated S-nitrosation through GSNOR plays important roles in cellular processes and signaling in plants, but the regulatory mechanism of programmed cell death by S-nitrosation is unclear. This study investigated the S-nitrosated proteomic and functions of GSNOR during Al-induced PCD in peanut. Al stress increased SNO content and GSNOR activity, with a significant positive correlation between SNO content and hydrogen peroxide content. S-nitrosated proteins were involved in regulating various biological processes, and the activity of GSNOR was inhibited by S-nitrosation.
Article
Biochemistry & Molecular Biology
Lijin Jiao, Ling-Yan Su, Qianjin Liu, Rongcan Luo, Xinhua Qiao, Ting Xie, Lu-Xiu Yang, Chang Chen, Yong-Gang Yao
Summary: The S-nitrosoglutathione reductase (GSNOR) is a key denitrosating enzyme that regulates protein S-nitrosation. Research has shown that GSNOR plays an important physiological role in Parkinson's disease (PD), controlling CDK5-mediated autophagy and contributing to the pathology.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Applied
Xiaozhao Wang, Jixuan Cao, Jin Qiao, Jing Pan, Song Zhang, Qingqing Li, Qingguo Wang, Biao Gong, Jingying Shi
Summary: This study found that treating cherry tomatoes with GABA can enhance their disease resistance by regulating GSNOR to maintain endogenous NO balance.
Article
Biochemistry & Molecular Biology
Elias Herrero-Galan, Ines Martinez-Martin, Cristina Sanchez-Gonzalez, Natalia Vicente, Elena Bonzon-Kulichenko, Enrique Calvo, Carmen Suay-Corredera, Maria Rosaria Pricolo, Angel Fernandez-Trasancos, Diana Velazquez-Carreras, Claudio Badia Careaga, Mahmoud Abdellatif, Simon Sedej, Peter P. Rainer, David Giganti, Raul Perez-Jimenez, Jesus Vazquez, Jorge Alegre-Cebollada
Summary: Titin's mechanical properties can be modulated by oxidative modifications of cysteines, which may contribute to the pathogenesis of heart disease.
Article
Fisheries
Yuhe Zhao, Yang Liu, Jianfen Wu, Dan Kong, Sifan Zhao, Guopan Li, Wei Li
Summary: The authors cloned the ALR gene from swamp eel and expressed and purified the recombinant protein. The rALR protein showed efficient reductive activity against various aldehydes, ketones, and GSNO. Administration of rALR in swamp eels increased hepatic GSH levels, decreased hepatic NO content and levels of reactive oxygen and nitrogen species, and regulated the expression of related genes, suggesting a role of ALR in response to nitrosative stress.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Narayani Nagarajan, Shin-ichi Oka, Jihoon Nah, Changgong Wu, Peiyong Zhai, Risa Mukai, Xiaoyong Xu, Sanchita Kashyap, Chun-Yang Huang, Eun-Ah Sung, Wataru Mizushima, Allen Sam Titus, Koichiro Takayama, Youssef Mourad, Jamie Francisco, Tong Liu, Tong Chen, Hong Li, Junichi Sadoshima
Summary: Modification of cysteine residues by oxidative and nitrosative stress affects protein structure and function, contributing to cardiovascular disease. Thioredoxin 1 (Trx1) acts as a denitrosylase or transnitrosylase, and in this study, it was found to transnitrosylate Atg7, promoting autophagy. During ischemia, Trx1 was oxidized and S-nitrosylated, and Atg7 reduced the disulfide bond in Trx1, allowing NO to be released and transferred to Atg7. Experiments with Atg7 C402S-knockin mice showed that S-nitrosylation of Atg7 at Cys402 promotes autophagy, protecting the heart against ischemia.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Behavioral Sciences
Yu-Xin Zhang, Qi-Yao Wei, Ya-Tao Wang, Li-Ping Zeng, Shi-Yu Sun, Yong-Fang Wu, Chong-Yang Ren, Fang Wang, Gui-Hai Chen, Lei Cao
Summary: This study found that gestational inflammation accelerated age-related learning and memory impairments, and postpartum exposure to EEs could alleviate these changes. These effects may be related to the expression of hippocampal TET1 and GSNOR.
BRAIN AND BEHAVIOR
(2022)
Article
Plant Sciences
Tereza Jedelska, Michaela Sedlarova, Jan Lochman, Lucie Cincalova, Lenka Luhova, Marek Petrivalsky
Summary: The regulation of protein function by reversible S-nitrosation has been identified as a key mechanism of NO signaling in plant development and stress responses. Through analyzing modulations of S-nitrosothiols and protein S-nitrosation mediated by S-nitrosoglutathione reductase in two different tomato genotypes infected with Phytophthora infestans and Phytophthora parasitica, it was found that protein S-nitrosation plays an important regulatory role in coordinating plant resistance mechanisms against hemibiotrophic pathogens.
HORTICULTURE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Rumi Mikami, Shunsuke Tsukagoshi, Yoshiki Oda, Kenta Arai
Summary: This study developed a new class of S-denitrosylase mimic, which promoted the denitrosylation of nitrosothiols and showed potential in inhibiting protein misfolding induced by S-nitrosylation. The structural analysis indicated that the hydrogen bond between the imidazole ring and selenol group may play a key role in enhancing catalytic activity.
Article
Biochemistry & Molecular Biology
Sana Qausain, Faez Iqbal Khan, Md Khurshid Alam Khan
Summary: 1-Cys peroxiredoxin6 (Prdx6) is a unique and inducible bifunctional enzyme in the mammalian lungs, playing a role in the progression and inhibition of cancer cells. It possesses two distinct active sites for phospholipase A2 and peroxidase activity. The conserved residues surrounding the peroxidase active site, known as second shell residues, include Glu50, Leu71, Ser72, His79, and Arg155. The study aimed to explore the role of Glu50 in the stabilization of the transition state of Prdx6 through mutation analysis and comparative analyses of biochemical, biophysical, and in silico methods. The results demonstrate that Glu50 significantly affects the structure, stability, and active site stabilization of Prdx6.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Erik G. Marklund, Yichen Zhang, Eman Basha, Justin L. P. Benesch, Elizabeth Vierling
CELL STRESS & CHAPERONES
(2018)
Review
Reproductive Biology
Damian D. Guerra, K. Joseph Hurt
BIOLOGY OF REPRODUCTION
(2019)
Article
Plant Sciences
Fionn McLoughlin, Minsoo Kim, Richard S. Marshall, Richard D. Vierstra, Elizabeth Vierling
Article
Multidisciplinary Sciences
Damian D. Guerra, Rachael Bok, Vibhuti Vyas, David J. Orlicky, Ramon A. Lorca, K. Joseph Hurt
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Pharmacology & Pharmacy
Damian D. Guerra, Rachael Bok, Ramon A. Lorca, K. Joseph Hurt
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Review
Plant Sciences
Elizabeth R. Waters, Elizabeth Vierling
Article
Plant Sciences
Junzhe Wang, Xiaolong Guo, Qiang Xiao, Jianchu Zhu, Alice Y. Cheung, Li Yuan, Elizabeth Vierling, Shengbao Xu
Summary: The nucellus tissue plays a crucial role in plant reproduction by influencing the degeneration of the nucellus and the expansion of the female gametophyte through the regulation of auxin. Auxin efflux is essential for controlling the precise distribution of auxin around the nucellus and the female gametophyte. Maternal tissues control auxin concentration and efflux, serving as a key communication between maternal and filial tissues during development.
Article
Plant Sciences
Tianxiang Liu, Jesse Arsenault, Elizabeth Vierling, Minsoo Kim
Summary: The research highlights the essential role of the mitochondrial ATP synthase d subunit in normal plant growth and heat stress tolerance. Downregulation of ATPd affects only subunits of the mitochondrial ATP synthase complex V, leading to increased sensitivity to heat stress, slower growth, and abnormal leaf morphology in plants.
Article
Biochemistry & Molecular Biology
Rachael Bok, Damian D. Guerra, Ramon A. Lorca, Sara A. Wennersten, Peter S. Harris, Abhishek K. Rauniyar, Sally P. Stabler, Kenneth N. MacLean, James R. Roede, Laura D. Brown, K. Joseph Hurt
Summary: The study revealed that cystathionine gamma-lyase (CSE) plays a crucial role in maintaining the glutathione (GSH)/glutathione disulfide (GSSG) balance in uterine arteries for nitric oxide signaling, which is necessary for normal estrogen (E-2) stimulation. These findings have implications for pregnancy pathophysiology and the selective hormone responses of specific vascular beds.
Correction
Plant Sciences
Alice Y. Cheung, Xiaolong Guo, Elizabeth Vierling, Junzhe Wang, Qiang Xiao, Shengbao Xu, Li Yuan
Article
Plant Sciences
Minsoo Kim, Vincent Schulz, Lea Brings, Theresa Schoeller, Kristina Kuehn, Elizabeth Vierling
Summary: The study found that mutations in the Arabidopsis thaliana mTERF18/SHOT1 gene enable plants to better tolerate heat and oxidative stresses, potentially due to lower ROS production in mitochondria. Additionally, the research revealed that the SHOT1 protein binds DNA and localizes to mitochondrial nucleoids, disruption of which affects the accumulation of oxidative phosphorylation complexes.
Review
Biochemistry & Molecular Biology
Patrick Treffon, Elizabeth Vierling
Summary: This review provides an overview of the biochemistry and regulation of nitro-oxidative modifications of protein Cys residues, focusing on NO production and S-nitrosation. It also discusses the importance and recent advances in understanding the enzymatic systems involved in regulating S-denitrosation.
Article
Biochemistry & Molecular Biology
Minsoo Kim, John Swenson, Fionn McLoughlin, Elizabeth Vierling
Summary: Studies revealed that heat shock protein 101 (HSP101) is essential for thermotolerance in plants, bacteria, and yeast. Through a suppressor screen in Arabidopsis thaliana, a mutation in the CLEAVAGE STIMULATION FACTOR77 (CstF77) gene was identified as a suppressor of hot1-4, suggesting its critical role in mRNA 3' end maturation. The dominant role of HSP101 in severe heat stress outcome was also demonstrated.
Article
Plant Sciences
Elizabeth R. Waters, Magdalena Bezanilla, Elizabeth Vierling
Summary: ATPase family AAA domain-containing 3 (ATAD3) proteins are unique mitochondrial proteins that are essential in metazoans and Arabidopsis thaliana. They play a critical role in mitochondrial protein quality control and their disruption leads to reduced growth and aberrant mitochondrial function in plants.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Lingling Zhu, Andrew P. Scafaro, Elizabeth Vierling, Marilyn C. Ball, Bradley C. Posch, Frederike Stock, Owen K. Atkin
Summary: The study investigates the response of Polyscias elegans, a tropical-subtropical rainforest tree species, to heat stress. The results show that within 2 hours of exposure to 40 degrees Celsius, the leaf heat tolerance increased along with the accumulation of metabolites and heat shock proteins. However, it took more than 2 days for the membrane lipid fatty acid composition to change. These findings highlight the importance of multi-faceted responses in plant survival during heat waves in the context of ongoing global climate change.