Journal
FRONTIERS IN NEUROSCIENCE
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnins.2019.00579
Keywords
photoacoustic; neuroimaging; near-infrared; voltage-sensitive dye; transcranial; seizure
Categories
Funding
- NIH Brain Initiative [R24MH106083-03]
- NIH National Institute of Biomedical Imaging and Bioengineering [R01EB01963]
- National Institute of Child Health & Human Development [R01HD90884]
- NIH National Institute of Heart, Lung, and Blood Institute [R01HL139543]
- Intuitive Surgical Technology
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2018R1A6A3A03011551]
- National Science Foundation [1653322]
- National Research Foundation of Korea [2018R1A6A3A03011551] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Div Of Information & Intelligent Systems
- Direct For Computer & Info Scie & Enginr [1653322] Funding Source: National Science Foundation
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Minimally-invasive monitoring of electrophysiological neural activities in real-time-that enables quantification of neural functions without a need for invasive craniotomy and the longer time constants of fMRI and PET-presents a very challenging yet significant task for neuroimaging. In this paper, we present in vivo functional PA (fPA) imaging of chemoconvulsant rat seizure model with intact scalp using a fluorescence quenching-based cyanine voltage-sensitive dye (VSD) characterized by a lipid vesicle model mimicking different levels of membrane potential variation. The framework also involves use of a near-infrared VSD delivered through the blood-brain barrier (BBB), opened by pharmacological modulation of adenosine receptor signaling. Our normalized time-frequency analysis presented in vivo VSD response in the seizure group significantly distinguishable from those of the control groups at sub-mm spatial resolution. Electroencephalogram (EEG) recording confirmed the changes of severity and frequency of brain activities, induced by chemoconvulsant seizures of the rat brain. The findings demonstrate that the near-infrared fPA VSD imaging is a promising tool for in vivo recording of brain activities through intact scalp, which would pave a way to its future translation in real time human brain imaging.
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