Journal
FRONTIERS IN PLANT SCIENCE
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2019.00880
Keywords
Atlantic cod nervous necrosis virus; virus-like particles; transient expression; sea bass; protective immunity
Categories
Funding
- UK Biotechnological and Biological Sciences Research Council (BBSRC) [BB/M027856/1]
- Institute Strategic Programme Grant Understanding and Exploiting Plant and Microbial Secondary Metabolism [BB/J004596/1]
- Institute Strategic Programme Grant Molecules from Nature - Enhanced Research Capacity [BBS/E/J/000PR9794]
- John Innes Foundation
- Ministry of Agriculture and Forestry [2145/312/2014]
- VTT
- Wellcome Trust [102572/B/13/Z]
- BBSRC [BB/M027856/1, BB/R001669/1] Funding Source: UKRI
Ask authors/readers for more resources
Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
