Article
Medicine, Research & Experimental
Yan Sun, Lang Xiong, Xiaoran Wang, Liping Wang, Baoxin Chen, Jingqi Huang, Mi Huang, Jieping Chen, Jing Wu, Shan Huang, Yizhi Liu
Summary: This study demonstrated that TGF-beta 2 induces autophagy flux during EMT progression of LECs, with autophagy activation enhancing fibrogenic responses and cell migration, while autophagy inhibition alleviates EMT markers and cell migration. Additionally, modulation of Smad signaling by autophagy was observed, and ATG7-silenced LECs showed antifibrosis effect induced by TGF-beta 2. Overall, intervention/inhibition of autophagy could attenuate TGF-beta 2-induced EMT in LECs, providing insights for preventing and treating fibrotic cataracts and other fibrotic diseases.
Article
Cell Biology
Mary Flokis, Frank J. Lovicu
Summary: FGF-2 and TGF-beta can regulate LEC behavior, with FGF-2 promoting fiber differentiation and TGF-beta inducing EMT. When used together, central LECs undergo EMT while peripheral LECs favor fiber differentiation. This differential regulation is important in the development of lens pathology.
Article
Cell & Tissue Engineering
Le Phuong Hoang Anh, Ken Nishimura, Akihiro Kuno, Nguyen Thuy Linh, Tetsuo Kato, Manami Ohtaka, Mahito Nakanishi, Eiji Sugihara, Taka-Aki Sato, Yohei Hayashi, Aya Fukuda, Koji Hisatake
Summary: Somatic cell reprogramming proceeds through a series of events to generate induced pluripotent stem cells (iPSCs). In this study, the researchers found that the downregulation of Osr2 is crucial for efficient reprogramming by using a cell-based model. Osr2 acts as a novel EMT regulator and its downregulation diminishes TGF-beta signaling and activates Wnt signaling, promoting MET and acquiring pluripotency.
Article
Cell Biology
Yuan Xiao, Jia-Wen Xiang, Qian Gao, Yue-Yue Bai, Zhao-Xia Huang, Xiao-Hui Hu, Ling Wang, David Wan-Cheng Li
Summary: Research has shown that Mab21L1 promotes survival of lens epithelial cells by regulating the expression of αB-crystallin and suppressing the ATR/CHK1/p53 pathway. This finding contributes to a better understanding of the functional mechanism of Mab21L1.
Article
Cell Biology
Lang Xiong, Yan Sun, Jingqi Huang, Pengjuan Ma, Xiaoran Wang, Jiani Wang, Baoxin Chen, Jieping Chen, Mi Huang, Shan Huang, Yizhi Liu
Summary: This study reveals the regulatory mechanism of lncRNA H19 in lens fibrosis. The researchers found that H19 was highly expressed in lens epithelial cells but downregulated after exposure to TGF-beta 2. Silencing H19 exacerbated TGF-beta 2-induced EMT, while overexpressing H19 partially reversed EMT. In vitro and in vivo experiments confirmed the important role of H19 in maintaining lens clarity.
Article
Pharmacology & Pharmacy
Minli Huang, Mengru Fu, Jia Wang, Chunhua Xia, Hong Zhang, Yuqing Xiong, Jiake He, Jianming Liu, Bingchen Liu, Siyi Pan, Fanglan Liu
Summary: Cancer-associated fibroblasts (CAFs) play a crucial role in breast cancer initiation, metastasis, and invasion. This study investigated the requirement of autophagy and FAP-alpha in breast cancer cell invasion and metastasis, finding that both are necessary. Targeting autophagy or FAP-alpha individually may be a potential approach to improve the prognosis of human breast cancer.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Cell Biology
Xi Chen, Hong Yan, Ying Chen, Guo Li, Yue Bin, Xiyuan Zhou
Summary: The study demonstrates that both TGF-beta/Smad and canonical Wnt/beta-catenin pathways play a significant role in regulating epithelial-mesenchymal transformation of lens epithelial cells but might be in a different stage.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Article
Biochemistry & Molecular Biology
Lauren A. Griggs, Christopher A. Lemmon
Summary: Epithelial to Mesenchymal Transition (EMT) is a process in which epithelial cells transition to a motile and invasive mesenchymal phenotype. The EMT process is associated with an increase in fibronectin (FN) assembly and the localization of Transforming Growth Factor-beta 1 (TGF-beta 1) to FN fibrils. This study investigated the spatial gradients of EMT and FN fibrillogenesis in mammary epithelial cells cultured on square micropatterns in response to TGF-beta 1. The results showed that increasing TGF-beta 1 concentration induced a spatial gradient of both E-Cadherin and FN fibrils, and inhibition of FN fibril assembly globally reduced both E-Cadherin-containing adherens junctions and FN fibrillogenesis, but did not eliminate the spatial gradients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Wan Hua, Sarantos Kostidis, Oleg Mayboroda, Martin Giera, Marten Hornsveld, Peter ten Dijke
Summary: TGF-beta induces metabolic reprogramming in epithelial cells during EMT, affecting glycolysis, TCA cycle, glutaminolysis, choline metabolism, cellular redox state, and other pathways. Inhibiting choline kinase alpha with a kinase inhibitor can attenuate TGF-beta-induced changes associated with EMT.
Review
Cell Biology
Aftab Taiyab, Judith West-Mays
Summary: Injury to the ocular lens disrupts cell-cell and cell-capsule/basement membrane interactions, leading to a series of interconnected signaling events. These events include cell-adhesion and growth factor-mediated signaling pathways that play a crucial role in the induction and progression of epithelial-mesenchymal transition (EMT) and fibrosis of lens epithelial cells. The lens provides a simple and ideal system to study injury-induced signaling pathways associated with EMT and fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Hyun-Wook Lee, Cynthia C. Jose, Suresh Cuddapah
Summary: Nickel compounds, prevalent in the atmosphere due to industrial processes and fossil fuel burning, are associated with chronic inflammatory lung diseases, including asthma and lung cancer, primarily through the mechanism of epithelial-mesenchymal transition (EMT).
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jia-Qi Huang, Hao Zhang, Xue-Wei Guo, Yan Lu, Si-Nian Wang, Bo Cheng, Su-He Dong, Xiao-Li Lyu, Feng-Sheng Li, Yong-Wang Li
Summary: This study demonstrates the critical role of PIEZO1 signaling in radiation-induced epithelial-mesenchymal transition (EMT) by forming a positive feedback loop with TGF-beta 1. Activation of PIEZO1 can upregulate TGF-beta 1 expression and promote EMT through the Ca2+/HIF-1 alpha signaling pathway, while knockdown of PIEZO1 or HIF-1 alpha partially reverses radiation-induced EMT. Additionally, the expression of PIEZO1 is regulated by the transcription factor C/EBP beta, which further influences the EMT process in response to radiation and TGF-beta 1.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Long Xu, Jian Wang, Buhan Liu, Jiaying Fu, Yuanxin Zhao, Sihang Yu, Luyan Shen, Xiaoyu Yan, Jing Su
Summary: Epithelial ovarian cancer has the highest mortality rate among gynecological malignant tumors, largely due to metastasis. This study found that HDAC9 plays different roles in different histological subtypes of ovarian cancer and may be a potential target for personalized treatment.
Article
Nutrition & Dietetics
Wei-Chung Chiou, Cheng Huang, Zi-Jun Lin, Lian-Sheng Hong, Yu-Heng Lai, Jui-Chieh Chen, Hsiu-Chen Huang
Summary: Alpha-viniferin and epsilon-viniferin show significant inhibitory effects on EMT, invasion, and migration in non-small cell lung cancer, and can reverse the expression changes induced by TGF-beta 1.