Review
Biochemistry & Molecular Biology
Alicja M. Kmiecik, Piotr Dziegiel, Marzenna Podhorska-Okolow
Summary: Cancer is heterogeneous, and the protein NUCB2/NESF-1 plays a crucial role in cancer development and progression. High expression of NUCB2/NESF-1 is associated with poor outcomes in various cancers and promotes cell proliferation, migration, and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Rafal Lenda, Michal Padjasek, Artur Krezel, Andrzej Ozyhar, Dominika Bystranowska
Summary: This study analyzed the effect of Zn(II) and Ca(II) on the structure of human nesfatin-1, -2, and -1/2. The results revealed that human nesfatin-1 is an intrinsically disordered protein, while nesfatin-2 and nesfatin-1/2 have globular structures with intrinsically disordered regions. Zn(II) treatment led to structurization and compaction of nesfatin-1, as well as destabilization of nesfatin-2 and nesfatin-1/2. Furthermore, nesfatin-1 and -2 seemed to be interdependent when linked together, as the properties of nesfatin-1/2 were not a simple sum of the properties exhibited by the individual peptides.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Oncology
Yunlai Liang, Yating Ma, Kun Wang, Manglin Xiang, Bin Yi
Summary: The association of NUCB-2/Nesfatin-1 with nasopharyngeal carcinoma was unclear, so this study aimed to clarify its role in the development, progression, and diagnosis of NPC. The results showed that NUCB-2 level was higher in NPC tissue than in rhinitis tissue, and suppression of NUCB-2 inhibited NPC cell proliferation while improvement of NUCB-2 promoted cell propagation. Moreover, serum NUCB-2 level could potentially be a serological marker for the early diagnosis of nasopharyngeal carcinoma.
CANCER CELL INTERNATIONAL
(2023)
Article
Cell Biology
Jasmine Hui, Gurpreet Kaur Aulakh, Suraj Unniappan, Baljit Singh
Summary: Deletion of the NUCB2/nesfatin-1 gene led to an increase in accumulation of adherent neutrophils in the lungs of NKO mice, suggesting an anti-inflammatory role of NUCB2/nesfatin-1 in acute lung inflammation.
CELL AND TISSUE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Aristea Psilopanagioti, Sofia Nikou, Souzana Logotheti, Marina Arbi, Dionysios V. Chartoumpekis, Helen Papadaki
Summary: The study focuses on the expression of GLP-1R in the human hypothalamus and its relationship with BMI. The results suggest that GLP-1R may play a role in regulating energy balance in the human hypothalamus, particularly in the LH region, which is associated with appetite and reward. The study also indicates a potential interaction between GLP-1R and nucleobindin-2/nesfatin-1 in signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Siyi Ning, Caiying Liu, Kangtao Wang, Yubo Cai, Zhicheng Ning, Ming Li, Liang Zeng
Summary: NUCB2/Nesfatin-1 promotes breast cancer metastasis by reprogramming cholesterol metabolism. NUCB2/Nesfatin-1 is overexpressed in breast cancer patients and is associated with poor prognosis. Mechanistically, NUCB2/Nesfatin-1 upregulates cholesterol synthesis via the mTORC1 signal pathway, contributing to breast cancer migration and metastasis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Physiology
Vi Pham, Joshua G. Pemberton, John P. Chang, Ayelen Melisa Blanco, Atefeh Nasri, Suraj Unniappan
Summary: This study provides evidence that nesfatin-1 is a stress responsive peptide which modulates stress axis hormones in fish. It was found that nesfatin-1 affects cortisol release, ACTH secretion, and mRNA levels of CRF in various tissues of goldfish under stress conditions. The presence of nucb2/nesfatin-1-like immunoreactivity in the hypothalamus, pituitary, and interrenal cells points to the potential role of nesfatin-1 in regulating the HPI axis.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Cell Biology
Anna Skorupska-Stasiak, Dominika Bystranowska, Jozef Ba Tran, Artur Krezel, Andrzej Ozyhar
Summary: This study investigated the structural and biochemical properties of the C-terminal half of Nucb2 and found that its product, nesfatin-3, had completely different properties from its precursor protein. Despite their similarities, nesfatin-3 is likely to play different roles in physiological processes and have different effects on tissue function, metabolism, and its regulation. The results of this study clearly demonstrated that nesfatin-3 possesses divalent metal ion binding properties that were not found in the nucleobindin-2 precursor protein.
CELL COMMUNICATION AND SIGNALING
(2023)
Review
Biochemistry & Molecular Biology
Saleh Rahmati, Behnam Mohammadi, Zahra Karimi-Mehr, David Robert Broom
Summary: The aim of this rapid review is to assess the impact of physical activity and exercise on NUCB2 gene expression and Nesfatin-1 concentration. Human studies show that Nesfatin-1 concentration either remains unchanged or increases after exercise training, with higher intensity and longer duration of exercise training leading to a greater increase. Animal studies using mouse models generally demonstrate increased tissue NUCB2/Nesfatin-1 with regular exercise training. However, there is a lack of research on the acute response and biopsy studies in humans, as well as investigations on the effects of acute exercise in animals. Comparative studies and further research are recommended to enhance our understanding of the effects of exercise on NUCB2/Nesfatin-1 in humans and animals.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Biochemistry & Molecular Biology
Sophia Kristina Rupp, Andreas Stengel
Summary: Studies have shown that NUCB2/nesfatin-1 is widely distributed in the brain and spinal cord and plays a role in autonomic regulation, with nesfatin-1 modulating autonomic functions in various regions. Additionally, the autonomic nervous system influences the activity of nesfatin-1 neurons, with the vagus nerve playing a crucial role in nesfatin-1 regulation. While data is still limited, research has clearly demonstrated the complex interplay between nesfatin-1 and the autonomic nervous system.
Article
Behavioral Sciences
Vida Bitarafan, Penelope C. E. Fitzgerald, Sally D. Poppitt, John R. Ingram, Christine Feinle-Bisset
Summary: Gastrointestinal functions, including pyloric motility and gut hormones, play a role in regulating acute energy intake. Bitter tastants can modulate these functions, but higher doses may cause gastrointestinal symptoms. This study evaluated the effects of dose and delivery location of a bitter hop extract on various gastrointestinal factors in healthy-weight men.
Article
Biochemistry & Molecular Biology
Murielle Abou-Samra, Koen Venema, Carole Ayoub Moubareck, Mirey Karavetian
Summary: This cross-sectional analysis examined the association of peptide hormones with obesity markers, insulin resistance, and dyslipidemia. The study found that obese individuals had higher levels of leptin, insulin, insulin resistance, and triglycerides, while normal-weight individuals had higher levels of ghrelin.
Article
Agriculture, Dairy & Animal Science
Jithine Jayakumar Rajeswari, Suraj Unniappan
Summary: The study found that nesfatin-1 and Nlp can stimulate insulin production in adipose tissue and modulate growth hormone and insulin-like growth factors and glucose transporters in goldfish. These results strongly support the tissue-specific regulatory roles of nesfatin-1 and Nlp in growth and glucose metabolism in goldfish.
Article
Gastroenterology & Hepatology
Zarife N. Ozdemir-Kumral, Turkan Koyuncuoglu, Sevil Arabaci-Tamer, Ozlem T. Cilingir-Kaya, Ayca K. Koroglu, Meral Yuksel, Berrak C. Yegen
Summary: High-fat diet induced mild systemic inflammation, disrupted enteric innervation, enhanced gastric contractility, inhibited ileal contractility, and eliminated the inhibitory effect of NES-1 on gastric motility.
JOURNAL OF NEUROGASTROENTEROLOGY AND MOTILITY
(2021)
Review
Biochemistry & Molecular Biology
Martha A. Schalla, Andreas Stengel
Summary: Ghrelin and nesfatin-1, as enteroendocrine peptide hormones, play crucial roles not only in food intake but also in various physiological systems, including the reproductive system. Research has shown their distribution along the hypothalamus-pituitary-gonadal (HPG) axis, their modulation by reproductive hormones, and their impact on reproductive functions. Further exploration is needed to fill the gaps in current knowledge.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)