Article
Cell Biology
Teka Khan, Arun S. Seetharam, Jie Zhou, Nathan J. Bivens, Danny J. Schust, Toshihiko Ezashi, Geetu Tuteja, R. Michael Roberts
Summary: The study reveals the complexity of trophoblast lineage emergence using the BAP model, showing distinct characteristics of trophoblast cells under different oxygen conditions. Transcriptomic and cluster analyses suggest potential fusion events and different gene expression patterns in syncytiotrophoblast and cytotrophoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Bianca Dietrich, Victoria Kunihs, Andreas I. Lackner, Gudrun Meinhardt, Bon-Kyoung Koo, Jurgen Pollheimer, Sandra Haider, Martin Knoefler
Summary: This study reveals that autocrine NOTCH3 signaling controls human placental development by promoting self-renewal of cytotrophoblast progenitors.
Review
Biochemistry & Molecular Biology
Rowan M. Karvas, Laurent David, Thorold W. Theunissen
Summary: Trophoblasts play critical roles in blastocyst implantation and maternal-fetal communication during pregnancy. However, our understanding of human trophoblast biology is limited due to the scarcity of first-trimester placental tissue and the inadequacy of animal models. Recent advancements in deriving human trophoblast stem cells from pluripotent and somatic cell sources provide exciting opportunities to study the molecular mechanisms of placental development and pregnancy-related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Joanna L. James, Abbey Lissaman, Yohanes N. S. Nursalim, Lawrence W. Chamley
Summary: The use of in vitro tools to study trophoblast differentiation and function is crucial for understanding placental development. Various models including primary trophoblasts, placental explants, stem cell models, and organoid cultures have provided insights into the complex microenvironment and cell-cell crosstalk involved in this process. However, it is important to consider the strengths and limitations of each model and accurately interpret the in vitro data in relation to in vivo aspects of human placentation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ruth Hornbachner, Andreas Lackner, Henrieta Papuchova, Sandra Haider, Martin Knoefler, Karl Mechtler, Paulina A. Latos
Summary: The study identified MSX2 as a key transcriptional regulator of trophoblast identity, which can prevent premature syncytiotrophoblast differentiation. MSX2 co-operates with the SWI/SNF complex to regulate multiple differentiation genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell & Tissue Engineering
Jiaxing Wang, Ping Long, Shengnan Tian, Weihua Zu, Jing Liu, Bangyong Wu, Jilong Mao, Dan Li, Yanlin Ma, Yuanhua Huang
Summary: Extravillous trophoblast (EVT) cells are crucial for maternal-fetal interaction, and their abnormal development and function are associated with severe pregnancy disorders. This study established a cell model by differentiating human-induced pluripotent stem cells and human embryonic stem cells into EVT cells and investigated the effects of the immunosuppressant Cyclosporin A (CsA) on the invasion and migration of these cells. The results suggest that CsA promotes the invasion and migration of EVT cells, providing insights into the mechanism of pregnancy-related disorders and offering a potential treatment option.
STEM CELLS AND DEVELOPMENT
(2023)
Review
Genetics & Heredity
Sehee Choi, Teka Khan, R. Michael Roberts, Danny J. Schust
Summary: Understanding the process of human placentation is crucial for the treatment of pregnancy complications. Animal and in vitro models, including stem cell-derived approaches and transcriptomic technologies, have been extensively used for studying human placentation. Recent advancements in induced pluripotent stem cells (iPSCs) have allowed for disease- and patient-specific research on pregnancy disorders. The application of RNA-seq and single nuclei RNA sequencing (snRNA-seq) technologies has improved our understanding of the transcriptome of the syncytiotrophoblast (STB) and its role in diseases like preeclampsia (PE).
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Jiaye Chen, Tinglin Song, Sizhu Yang, Qingxue Meng, Xiaoyu Han, Ze Wu, Jung-Chien Cheng, Lanlan Fang
Summary: This study aimed to investigate the effect of GDF-8 on invasion and MMP2 expression in human EVT cells, and found that Snail is a key factor in GDF-8-induced MMP2 expression and cell invasion.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Developmental Biology
Robert Morey, Tony Bui, Kathleen M. Fisch, Mariko Horii
Summary: This article describes in vitro models of human trophoblast differentiation, including primary and hPSC-derived TSC as well as trophoblast organoids. These models help study early placental development and diseases in various settings.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Obstetrics & Gynecology
Zahra Anvar, Imen Chakchouk, Momal Sharif, Sangeetha Mahadevan, Li Su, Swathi Anikar, Fatemeh Alavi Naini, Alloysius Budi Utama, Ignatia B. van den Veyver
Summary: Differentiation of human embryonic stem cells (hESCs) into trophoblast-like cells can be achieved by culturing them in media containing bone morphogenic protein 4 (BMP4). Various media containing BMP4 were compared, and it was found that they differentially drove early differentiation towards trophoblast-like lineages. Each media had its advantages and disadvantages, and selection of a protocol for specific experiments needs to consider these factors.
REPRODUCTIVE SCIENCES
(2023)
Article
Cell Biology
Robert Morey, Omar Farah, Sampada Kallol, Daniela F. Requena, Morgan Meads, Matteo Moretto-Zita, Francesca Soncin, Louise C. Laurent, Mana M. Parast
Summary: During pregnancy, the EVT invades and remodels maternal blood vessels to ensure blood supply to the placenta, exhibiting polyploidy and senescence in early gestation. Comprehensive profiling of genomic organization and transcriptome shows that both first trimester and term EVT undergo senescence, are primarily tetraploid, and lack high rates of copy number variations. Genes and pathways related to senescence and polyploidy are important for normal EVT differentiation and maturation, with a key role identified for the unfolded protein response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Jiaying Qin, Wanqiong Li, Bo Lv, Zhigang Xue, Jinfeng Xue
Summary: During embryo development, the totipotent blastomere undergoes the first lineage decision, resulting in the formation of the inner cell mass (ICM) and the trophectoderm (TE). The ICM develops into the fetus, while the TE forms the placenta, which acts as the interface between maternal and fetal bloodstreams. Proper differentiation of trophoblast lineage is crucial for placental and fetal development, and abnormalities in this process are associated with pregnancy disorders and fetal growth restriction. This review focuses on the early differentiation and key regulatory factors of trophoblast lineage, which have been poorly understood. Additionally, recent developments in trophoblast stem cells, trophectoderm stem cells, and blastoids provide accessible models to study embryo implantation and placentation.
BIOLOGY OF REPRODUCTION
(2023)
Review
Cell Biology
Adam Jaremek, Mariyan J. Jeyarajah, Gargi Jaju Bhattad, Stephen J. Renaud
Summary: Proper development of the placenta, specifically the syncytiotrophoblast, is crucial for successful pregnancy as it regulates the exchange of nutrients and gases between maternal and fetal blood. Dysregulated formation of syncytiotrophoblast can have negative impacts on maternal and fetal health. 'Omics' approaches have been used to gain multidimensional insights into the formation and function of syncytiotrophoblast, but there are still limitations and future avenues for exploration in this area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Fisheries
Zhuosi Li, Yutaka Shimizu, Hiroki Saeki
Article
Biochemistry & Molecular Biology
Jiawen Zheng, Zhuosi Li, Yuki Manabe, Minji Kim, Tsuyoshi Goto, Teruo Kawada, Tatsuya Sugawara
Article
Nutrition & Dietetics
Zhuo-Si Li, Kenji Noda, Eriko Fujita, Yuki Manabe, Takashi Hirata, Tatsuya Sugawara
JOURNAL OF NUTRITION
(2015)
Review
Chemistry, Medicinal
Tatsuya Sugawara, Ponesakki Ganesan, Zhuosi Li, Yuki Manabe, Takashi Hirata
Article
Biochemistry & Molecular Biology
Zhuosi Li, Osamu Kurosawa, Hiroo Iwata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Nutrition & Dietetics
Zhuo-Si Li, Jia-Wen Zheng, Yuki Manabe, Takashi Hirata, Tatsuya Sugawara
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Yuki Manabe, Misato Ichihara, Kyoko Fukuda, Nami Tomonaga, Zhuo-Si Li, Yoshihide Yamanashi, Hiroshi Suzuki, Tappei Takada, Michinori Matsuo, Tatsuya Sugawara
Article
Biochemistry & Molecular Biology
Zhuosi Li, Osamu Kurosawa, Hiroo Iwata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Chemistry, Medicinal
Zhuosi Li, Jiawen Zheng, Xiaolin Luo, Yuki Manabe, Takashi Hirata, Tatsuya Sugawara
Article
Cell & Tissue Engineering
Zhuosi Li, Osamu Kurosawa, Hiroo Iwata
TISSUE ENGINEERING PART A
(2020)
Article
Food Science & Technology
Tianmei Sun, Yangtai Liu, Xiaojie Qin, Zafeiro Aspridou, Jiaming Zheng, Xiang Wang, Zhuosi Li, Qingli Dong
Summary: This study evaluated the prevalence and epidemiology of Salmonella contamination in raw poultry meat commercialized in China. Different prevalence levels were found among different categories of poultry meat, with chilled poultry meat being more susceptible to cross-contamination. Higher prevalence levels of Salmonella in raw poultry meat were identified in Shaanxi, Henan, Sichuan, and Beijing regions.