Article
Polymer Science
Ok-Hyeon Kim, Jun-Hyung Park, Jong-In Son, Ok-Ja Yoon, Hyun-Jung Lee
Summary: The study demonstrates that silk fibroin scaffolds can enhance the anti-inflammatory and immunomodulatory functions of mesenchymal stromal cells, showing potential benefits for the treatment of inflammatory disorders.
Review
Biochemistry & Molecular Biology
Giovanni Schepici, Agnese Gugliandolo, Emanuela Mazzon
Summary: Neurological diseases are one of the main causes of disability in human life, making it necessary to investigate new strategies for improving the quality of life in neurological patients. This review focuses on preconditioning MSC therapy as a potential application to major neurological diseases, summarizing both in vitro and in vivo studies that demonstrate the efficacy of MSC preconditioning on neuronal regeneration and cell survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Luise Mueller, Antje Tunger, Manja Wobus, Malte von Bonin, Russell Towers, Martin Bornhaeuser, Francesco Dazzi, Rebekka Wehner, Marc Schmitz
Summary: Mesenchymal stromal cells (MSCs) have the ability to regulate and inhibit the function of immune cells, playing a crucial role in the pathogenesis of inflammatory disorders and showing promise in the treatment of conditions such as acute graft-versus-host disease, graft rejection, and autoimmune diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Mariana A. Antunes, Cassia L. Braga, Taina B. Oliveira, Jamil Z. Kitoko, Ligia L. Castro, Debora G. Xisto, Mariana S. Coelho, Nazareth Rocha, Rodrigo P. Silva-Aguiar, Celso Caruso-Neves, Eduarda G. Martins, Clara Fernandes Carvalho, Antonio Galina, Daniel J. Weiss, Jose R. Lapa e Silva, Miqueias Lopes-Pacheco, Fernanda F. Cruz, Patricia R. M. Rocco
Summary: The study compared MSCs from healthy and emphysematous donors, and observed their therapeutic effects in a mouse model of severe emphysema. MSCs from emphysematous donors exhibited gene downregulation and mitochondrial abnormalities, leading to inferior therapeutic outcomes compared to MSCs from healthy donors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jonathan O. Martinez, Michael Evangelopoulos, Ava A. Brozovich, Guillermo Bauza, Roberto Molinaro, Claudia Corbo, Xuewu Liu, Francesca Taraballi, Ennio Tasciotti
Summary: The study introduces a versatile multicomponent delivery platform that combines MSC tropism with MSV-mediated payload delivery, demonstrating increased drug release and reduced inflammation, resulting in improved outcomes in experimental models.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Cell Biology
Candice Bohaud, Rafael Contreras-Lopez, Jholy de la Cruz, Claudia Terraza-Aguirre, Mingxing Wei, Farida Djouad, Christian Jorgensen
Summary: Osteoarthritis is a multifaceted degenerative and inflammatory joint disorder, with current therapies often only providing short-term benefits without complete structural and functional reconstitution. Identifying novel therapeutic targets and strategies is crucial to achieve long-term clinical benefits for OA patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rosalia Crupi, Salvatore Cuzzocrea
Summary: Chronic inflammatory processes play a role in disease progression, particularly in atherosclerosis. Targeted anti-inflammatory treatment shows promise in reducing cardiovascular risk. Long-chain omega-3 polyunsaturated fatty acids (PUFAs) such as EPA have cardioprotective effects and beneficial effects on atherosclerotic plaques.
Article
Engineering, Biomedical
Jiabin Zhang, Ming Zhang, Rongcai Lin, Yuguang Du, Liming Wang, Qingqiang Yao, Andrew Zannettino, Hu Zhang
Summary: Stem cell therapy using magnetic scaffolds has been developed to treat osteoarthritis. The magnetic scaffolds enhance chondrogenesis of mesenchymal stem cells through mechanical stimulation and magnetic field effects. The fiber topography of the scaffolds can be tuned with different coatings on magnetic nanoparticles, which selectively up-regulate chondrogenesis or osteogenesis-related genes.
Review
Biochemistry & Molecular Biology
Raquel Guillamat-Prats
Summary: MSCs play a vital role in controlling various phases in response to injuries, immune reactions, wound healing, and regeneration. They can regulate coagulation pathways, trigger platelet production, and contribute to platelet activation. Proper characterization of MSCs is important to prevent adverse coagulation events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Critical Care Medicine
Amin M. Ektesabi, Keisuke Mori, James N. Tsoporis, Chirag M. Vaswani, Sahil Gupta, Chris Walsh, Amir K. Varkouhi, Shirley H. J. Mei, Duncan J. Stewart, W. Conrad Liles, John C. Marshall, Pingzhao Hu, Thomas G. Parker, Claudia C. dos Santos
Summary: The study showed that MSC therapy can regulate the production of host miRNAs, protecting cardiomyocytes from sepsis-induced myocardial dysfunction. By modulating miRNA expression, MSC therapy reduced inflammatory and apoptotic pathways activity, while increasing cardiac-specific structural and functional gene expression.
Article
Pharmacology & Pharmacy
Puneet Kaur, Jin Gao, Zhenjia Wang
Summary: Dietary omega 3 polyunsaturated fatty acids (PUFAs), including DHA and EPA, have beneficial effects on cardiovascular diseases and cancer due to their anti-inflammatory properties. This study introduces a novel liposomal formulation of EPA (EPA-liposomes) that shows significant reduction in inflammation-related molecules, providing potential therapeutic applications.
Article
Plant Sciences
Jae-Hun Ahn, Eun-Jung Song, Do-Hyeon Jung, Yeong-Jun Kim, In-Su Seo, Seong-Chan Park, You-Seok Jung, Eun-Seo Cho, Sang Hyun Mo, Jung Joo Hong, Jeong-Yong Cho, Jong-Hwan Park
Summary: The sesquiterpene lactone estafiatin derived from Artemisia scoparia was found to have anti-inflammatory effects on LPS-stimulated macrophages and protect mice from sepsis.
Article
Veterinary Sciences
Jun Ying, Qing You, Zhiguo Wang, Zhiqian Hu
Summary: This study suggests that preconditioning mesenchymal stem cells under hypoxic conditions can enhance their therapeutic effect in treating inflammatory bowel disease.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Endocrinology & Metabolism
Qiong Wu, Shuangli He, Yu Zhu, Shiming Pu, Zuping Zhou
Summary: This study demonstrated the effectiveness of adipose-derived stromal/stem cells (ASCs) in treating obesity and aging in aged mice. ASCs treatment led to significant weight loss, improved organ function, increased antioxidant capacity, enhanced lipid metabolism, and reduced inflammation in old mice. The positive effects of ASCs suggest their potential as primary antiaging and antiobesity agents for the elderly.
Review
Veterinary Sciences
Benjamin Uberti, Anita Plaza, Claudio Henriquez
Summary: Mesenchymal stem/stromal cells (MSCs) therapy is an important part of regenerative medicine and can modulate immune and inflammatory responses. However, there are discrepancies between the effects of MSCs in clinical applications and the results in experimental models, possibly due to limited survival or impaired function of cells in the inflammatory environment. Pre-conditioning strategies can enhance the survival and efficacy of MSCs.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Anesthesiology
Alice Marguerite Conrad, Gregor Loosen, Christoph Boesing, Manfred Thiel, Thomas Luecke, Patricia R. M. Rocco, Paolo Pelosi, Joerg Krebs
Summary: In patients with severe ARDS managed with V-V ECMO, increasing extracorporeal blood flow alters the calculation of lung water index (EVLWI) and intrathoracic blood volume index (ITBVI), resulting in unreliable measurements.
JOURNAL OF CLINICAL MONITORING AND COMPUTING
(2023)
Article
Pharmacology & Pharmacy
Mafalda Bacalhau, Filipa C. Ferreira, Arthur Kmit, Felipe R. Souza, Veronica D. da Silva, Andre S. Pimentel, Margarida D. Amaral, Camilla D. Buarque, Miqueias Lopes-Pacheco
Summary: In this study, a collection of triazole compounds was screened to identify novel F508del-CFTR correctors. Four hit compounds, LSO-18, LSO-24, LSO-28, and LSO-39, were identified and shown to rescue F508del-CFTR processing, plasma membrane trafficking, and function. The mechanism of action of these compounds was investigated and it was found that LSO-18, LSO-24, and LSO-28 exhibited additive effects with the clinically approved drugs VX-661 and VX-445.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Respiratory System
Hugo Goulart de Oliveira, Amarilio Macedo-Neto, Mauricio Saueressig, Paulo Sanches, Lea Fialkow, Edino Parolo, Fabiano Nagel, Tiago Garcia, Patricia Rieken Macedo Rocco
Summary: This study aimed to demonstrate whether a new prototype implantable artificial bronchus (IAB) can release trapped air from the lungs of recently deceased patients with emphysema. By mechanically ventilating seven deceased patients and measuring ventilation parameters and fraction of inspired oxygen before and after IAB placement, it was found that IAB implantation significantly reduced respiratory system plateau pressure and intrinsic positive end-expiratory pressure in most patients with emphysema. These findings provide a basis for further clinical studies on the safety and efficacy of IAB in patients with emphysema and its short- and long-term effects.
Article
Medicine, General & Internal
Sergio Augusto Lopes de Souza, Roberta Silveira Santos Laurindo, Gabriel Gutfilen-Schlesinger, Felippe Felix, Jose Luiz de Medeiros Amarante, Bianca Gutfilen
Summary: This study aimed to evaluate the sensitivity and specificity of Tc-99m-mononuclear leukocyte scintigraphy associated with Tc-99m-phytate in suspected Necrotizing External Otitis (NEO) patients, and found that Tc-99m-mononuclear leukocyte scintigraphy has high specificity and is the best option for NEO.
Article
Cell & Tissue Engineering
Soraia Abreu, Leonardo Alves, Luiza Carvalho, Debora Xisto, Natalia Blanco, Ligia Castro, Priscilla Olsen, Jose Roberto Lapa e Silva, Marcelo Marcos Morales, Miqueias Lopes-Pacheco, Daniel Weiss, Patricia Rieken Macedo Rocco
Summary: This study aims to evaluate the therapeutic effect of serum preconditioning on human mesenchymal stromal cells (hMSCs) in patients with asthma. The results show that serum preconditioning increases the apoptosis rate of hMSCs and the expression of transforming growth factor-β, interleukin-10, tumor necrosis factor-α-stimulated gene 6 protein, and indoleamine 2,3-dioxygenase-1. Serum preconditioning also promotes M2 polarization of macrophages and enhances their phagocytic activity, thereby reducing inflammation and remodeling.
Article
Infectious Diseases
Patricia Sola Penna, Sergio Augusto Lopes De Souza, Paulo Gustavo Limeira Nobre De Lacerda, Izabela Jardim Rodrigues Pitta, Clarissa Neves Spitz, Ana Maria Sales, Flavio Alves Lara, Ana Caroline Siquara De Souza, Euzenir Nunes Sarno, Roberta Olmo Pinheiro, Marcia Rodrigues Jardim
Summary: This study used 18F-FDG PET/CT to evaluate the peripheral nerves of nine leprosy patients and found that the uptake of 18F-FDG correlates with clinical neuritis. The results suggest that 18F-FDG PET-CT could be a useful tool to confirm neuritis, especially in difficult-to-diagnose cases.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Review
Pharmacology & Pharmacy
Miqueias Lopes-Pacheco, Patricia R. M. Rocco
Summary: Respiratory diseases pose significant health concerns worldwide, with high morbidity and mortality rates and considerable burdens on patients. Despite advances in understanding the pathologic mechanisms, current therapies for severe respiratory diseases are predominantly supportive and cannot improve lung function or reverse tissue remodeling. Mesenchymal stromal cells (MSCs) have shown promise in promoting immunomodulation, tissue repair, and other therapeutic properties in experimental models, but have had limited efficacy in early-stage clinical trials. Functional enhancement strategies, such as genetic engineering and preconditioning methods, have emerged to enhance the therapeutic actions of MSCs and improve clinical outcomes. This review discusses various strategies investigated in experimental settings to potentiate the therapeutic properties of MSCs for respiratory diseases, including changes in culture conditions, exposure to inflammatory environments, pharmacological agents, and genetic manipulation.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Respiratory System
Monique Martins Melo, Fernanda Ferreira Cruz, Patricia Rieken Macedo Rocco
Summary: Cell therapy has emerged as an alternative option for chronic lung diseases with high morbidity and mortality rates worldwide. This review discusses the definition and properties of mesenchymal stromal cells (MSCs), their mechanisms of action, as well as preclinical and clinical studies in various chronic lung diseases. Ongoing clinical trials are also presented.
EXPERT REVIEW OF RESPIRATORY MEDICINE
(2023)
Review
Medicine, General & Internal
Denise Battaglini, Brigitta Fazzini, Pedro Leme Silva, Fernanda Ferreira Cruz, Lorenzo Ball, Chiara Robba, Patricia R. M. Rocco, Paolo Pelosi
Summary: Over the last decade, management of acute respiratory distress syndrome (ARDS) has advanced significantly in terms of supportive and pharmacologic therapies. Lung protective mechanical ventilation is crucial for ARDS management, with recommendations including low tidal volume, plateau pressure, and driving pressure. Other therapies such as recruitment maneuvers and prone positioning are considered for severe ARDS cases. Despite extensive research, effective pharmacotherapies for ARDS are yet to be found, but sub-phenotypes of ARDS have shown potential for personalized pharmacologic treatments.
JOURNAL OF CLINICAL MEDICINE
(2023)
Letter
Respiratory System
L. Z. Salomao, R. A. Athanazio, S. Z. Rached, M. Lopes-Pacheco, M. Camargo
Review
Chemistry, Medicinal
Mafalda Bacalhau, Mariana Camargo, Grace A. V. Magalhaes-Ghiotto, Sybelle Drumond, Carlos Henrique M. Castelletti, Miqueias Lopes-Pacheco
Summary: Cystic fibrosis is a potentially fatal monogenic disease that has been significantly improved by the introduction of CFTR modulator drugs, particularly the triple combination therapy of elexacaftor, tezacaftor, and ivacaftor (ETI), which has shown safety and efficacy in reducing various disease symptoms. However, close monitoring by a healthcare team is necessary due to possible adverse effects associated with ETI therapy. This review aims to discuss the therapeutic benefits and adverse effects of ETI therapy for cystic fibrosis patients.
Article
Anesthesiology
Christoph Boesing, Laura Schaefer, Jochen Johannes Schoettler, Alena Quentin, Grietje Beck, Manfred Thiel, Patrick Honeck, Karl-Friedrich Kowalewski, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: This study compares the effects of different individualized PEEP titration strategies during Trendelenburg position with pneumoperitoneum. The results show that PEEP Delta P strategy can reduce lung stress and strain, and improve gas exchange. However, PEEP Delta P may result in decreased cardiac output.
EUROPEAN JOURNAL OF ANAESTHESIOLOGY
(2023)
Review
Engineering, Biomedical
Denise Battaglini, Ida Giorgia Iavarone, Chiara Robba, Lorenzo Ball, Pedro Leme Silva, Patricia R. M. Rocco
Summary: Despite extensive research, treatment for acute respiratory distress syndrome (ARDS) remains mainly supportive. New ventilatory modes and strategies have been proposed, but their clinical impact is uncertain and not supported by guidelines. This review provides an overview and update on ventilatory management for ARDS patients.
EXPERT REVIEW OF MEDICAL DEVICES
(2023)
Article
Physiology
Jakob Wittenstein, Martin Scharffenberg, Xiuli Yang, Thomas Bluth, Thomas Kiss, Marcus J. Schultz, Patricia R. M. Rocco, Paolo Pelosi, Marcelo Gama de Abreu, Robert Huhle
Summary: Global and regional transpulmonary pressure and driving pressure increase during protective low tidal volume one-lung ventilation compared to two-lung ventilation, and vary with body position. Lung instability is more likely to occur in the lateral and semilateral decubitus positions during one-lung ventilation in pigs after thoracic surgery.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Critical Care Medicine
Christoph Boesing, Laura Schaefer, Peter T. Graf, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: In ARDS patients treated with VV ECMO, adjusting PEEP to 10 cmH(2)O can reduce MP, improve hemodynamic parameters, and increase oxygen delivery.
JOURNAL OF CRITICAL CARE
(2024)