Journal
ACS MEDICINAL CHEMISTRY LETTERS
Volume 10, Issue 10, Pages 1393-1399Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsmedchemlett.9b00310
Keywords
Antibody-drug conjugates; maytansinoid; peptide
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Funding
- ImmunoGen, Inc.
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A new type of antibody-drug conjugate (ADC) has been prepared that contains a sulfur-bearing maytansinoid attached to an antibody via a highly stable tripeptide linker. Once internalized by cells, proteases in catabolic vesicles cleave the peptide of the ADC's linker causing self-immolation that releases a thiol-bearing metabolite, which is then S-methylated. Conjugates were prepared with peptide linkers containing only alanyl residues, which were all L isomers or had a single D residue in one of the three positions. A D-alanyl residue in the linker did not significantly impair a conjugate's cytotoxicity or bystander killing unless it was directly attached to the immolative moiety. Increasing the number of methylene units in the maytansinoid side chain of a conjugate did not typically affect an ADC's cytotoxicity to targeted cells but did increase bystander killing activity. ADCs with the highest in vitro bystander killing were then evaluated in vivo in mice, where they displayed improved efficacy compared to previously described types of maytansinoid conjugates.
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