Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 470, Issue 4, Pages 936-940Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2016.01.158
Keywords
Ubiquitination; Proteolysis; Proteasome; PROTAP; Bcl-xL
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Funding
- National Natural Science Foundation of China [21302108, 21572115]
- Shenzhen Municipal government [JSGG20141016150327538, CXB201104210014A]
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Ubiquitination proteasome pathway (UPP) is the most important and selective way to degrade proteins in vivo. Here, a novel proteolysis targeting peptide (PROTAP) strategy, composed of a target protein binding peptide, a linker and a ubiquitin E3 ligase recognition peptide, was designed to recruit both target protein and E3 ligase and then induce polyubiquitination and degradation of the target protein through UPP. In our study, the PROTAP strategy was proved to be a general method with high specificity using Bcl-xL protein as model target in vitro and in cells, which indicates that the strategy has great potential for in vivo application. (C) 2016 Elsevier Inc. All rights reserved.
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