Review
Infectious Diseases
Sabrina Lunara Santos Pavelquesi, Ana Carolina Almeida de Oliveira Ferreira, Angeislenie Ricelle Magalhaes Rodrigues, Calliandra Maria de Souza Silva, Daniela Castilho Orsi, Izabel Cristina Rodrigues da Silva
Summary: The study revealed high resistance rates of Salmonella to tetracyclines and sulfonamides, with poultry and pork being the most frequent samples used to isolate Salmonella. The most frequently detected tetracycline resistance genes in Salmonella were tetA and tetB, while sul1 was the most common sulfonamide resistance gene. These genes are associated with plasmids, transposons, or both, and have potential for transfer to other bacteria, environments, animals, and humans.
Article
Immunology
Soyeon Park, Bogyo Jung, Eunsuk Kim, Hyunjin Yoon, Tae-Wook Hahn
Summary: Non-typhoidal Salmonella (NTS) is a major cause of foodborne gastroenteritis and can cause invasive infection in humans. A live attenuated vaccine candidate, Delta fruR, was constructed and shown to provide 100% protection against NTS infection in mice. The Delta fruR mutant stimulated antibody production and reduced virulence in infected mice.
Article
Food Science & Technology
Zhen Zhang, Yuanyuan Zhao, Xueqin Chen, Wei Li, Wen Li, Jianming Du, Li Wang
Summary: This study investigated the effects of cinnamon essential oil (CEO) on Salmonella enteritidis cells. The results showed that CEO treatment had an obvious oxidative damaging effect on Salmonella enteritidis, leading to an increase in reactive oxygen species (ROS) content and disruption of the cellular antioxidant enzymes system, ultimately inhibiting bacterial growth. It was speculated that the antibacterial mechanism of CEO was the result of multiple effects.
Review
Biochemical Research Methods
Laura G. Leff, Kolapo Fasina, Jean Engohang-Ndong
Summary: Streams and rivers are impacted by human activities, leading to pollution and antibiotic resistance gene occurrence. Current methods of detecting antibiotic resistance genes have limitations, but the use of metagenomics can overcome these barriers. The public health and ecological impacts of antibiotic resistance are understudied.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Immunology
Yosef Daniel Huberman, Melanie Caballero-Garcia, Rober Rojas, Silvia Ascanio, Leandro Hipolito Olmos, Rosana Malena, Jorgelina Lomonaco, Paula Nievas, Paula Chero, Julio Levano-Gracia, Alfredo Mendoza-Espinoza
Summary: Poultry infections by Salmonella cause significant economic losses worldwide. Poultry serves as a common reservoir of Salmonella, posing a risk to humans through the food chain. Vaccination of layers plays an important role in preventing Salmonella infections.
Article
Agriculture, Dairy & Animal Science
E. A. Kimminau, T. P. Karnezos, K. N. Russo, J. A. Baxter, R. D. Berghaus, M. Jones, C. L. Hofacre
Summary: The study found that dietary bacteriophages do not interfere with the colonization or protection provided by the live Salmonella vaccine. There were no significant differences in Salmonella prevalence in cloacal swabs and cecal cultures among all treatment groups, but T2 and T3 had lower mortality and feed conversion ratio compared to T1.
Article
Microbiology
Carlotta Lauteri, Francesca Maggio, Annalisa Serio, Anna Rita Festino, Antonello Paparella, Alberto Vergara
Summary: The study evaluated the antibiotic resistance and synergistic effects of essential oils on 36 Salmonella strains, showing that Corydothymus capitatus essential oil displayed the best bioactivity in terms of inhibiting bacterial growth.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Alexander Meleshko, Nadzeya Piatrouskaya, Katsiaryna Vashkevich, Dzmitry Lutskovich, Chuan Wang, Dmitri Dormeshkin, Natalia Savelyeva, Mikalai Katsin
Summary: This study examined the use of intramuscular DNA-PEI vaccination followed by an oral Salmonella vaccine boost in lymphoma patients, which was found to be safe and well tolerated. The majority of patients showed T-cell immune responses to the vaccine rather than antibody responses. Out of the vaccinated patients, 6 had disease stabilization and 13 were in complete remission.
Article
Environmental Sciences
Zengzeng Fan, Sheng Yang, Qingyuan Zhu, Xuezhu Zhu
Summary: The individual and combined effects of tetracycline and divalent copper on activated sludge systems were studied in this research. The addition of tetracycline did not affect system performance, while the addition of copper inhibited biological phosphorous removal. Oxygen conditions had opposite effects on the abundances of high-risk TRGs.
Article
Immunology
Khandra T. Sears, Shamima Nasrin, Scott M. Baliban, Danielle N. Council, Marcela F. Pasetti, Sharon M. Tennant
Summary: Nontyphoidal Salmonella enterica is a common pathogen in food and particularly affects infants. The study evaluated the immunogenicity and protective efficacy of three live attenuated S. Typhimurium vaccines in mice. The results showed that oral immunization had poorer protective capacity and further improvements are needed for suitable protection in infants.
Article
Immunology
Javier Ochoa-Reparaz, Eduard Sebastia, Marta Sitja, Ibai Tamayo, Juan Manuel Irache, Carlos Gamazo
Summary: Researchers have developed a new method for a salmonellosis vaccine, with HE nanoparticles effectively reducing Salmonella excretion in hens. This approach shows potential for use in salmonellosis prevention and control on farms.
Article
Immunology
Fan Zhang, Emily M. Boerth, Joyce Gong, Nicole Ma, Katherine Lucas, Olivia Ledue, Richard Malley, Ying-Jie Lu
Summary: The generation of a bivalent vaccine against Vi and OSP using the Multiple Antigen Presenting System (MAPS) shows promising results in protecting against Salmonella Typhi and Paratyphi A infections.
Article
Microbiology
Penghao Lv, Xuesong Zhang, Mengze Song, Guijuan Hao, Fangkun Wang, Shuhong Sun
Summary: In this study, we constructed a recombinant Bacillus subtilis that can secrete a multi-epitope protein. Oral immunization of chickens with this recombinant B. subtilis induced high levels of specific IgG and specific secretory immunoglobulin A (sIgA), as well as increased levels of IL-2 and T cell-mediated immunity. This recombinant B. subtilis effectively protected chickens against S. Enteritidis and reduced pathological damage to the spleen and jejunum, indicating its potential as a mucosal vaccine candidate for animals to defend against S. Enteritidis in the future.
VETERINARY MICROBIOLOGY
(2023)
Article
Environmental Sciences
Jili Guo, Manhong Huang, Lijun Meng, Nan Jiang, Shengyang Zheng, Mengyu Shao, Xubiao Luo
Summary: The study developed a novel FO membrane for efficient removal of Cu2+, TC, and TRGs micropollutants in aquaculture wastewater. Factors such as Cu2+ concentration, complexation reaction, electrostatic interaction, and adsorption were found to play key roles in achieving high rejection efficiencies.
ENVIRONMENTAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Chen-Si Lin, Tsung-Lin Lu, Yi-An Chen, Hsin-Yi Yu, Chiu-Yi Wu, Wen-Yuan Yang
Summary: The study demonstrated that a commercial bivalent live attenuated vaccine showed desirable safety and effectiveness in SPF chickens and field layers, effectively preventing Salmonella infections and reducing flock mortality while improving egg production. Vaccination significantly reduced Salmonella cloacal shedding and viable cell counts, indicating a positive impact on Salmonella control.
Article
Biochemistry & Molecular Biology
Lillian G. Acuna, M. Jose Barros, Paula Nunez, Diego Penaloza, Fernanda Montt, Diego Pedraza, Katherine Crossley, Fernando Gil, Juan A. Fuentes, Ivan L. Calderon
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Alexander Carreno, Dayan Paez-Hernandez, Plinio Cantero-Lopez, Cesar Zuniga, Jan Nevermann, Angelica Ramirez-Osorio, Manuel Gacitua, Poldie Oyarzun, Felipe Saez-Cortez, Ruben Polanco, Carolina Otero, Juan A. Fuentes
Article
Microbiology
R. Alvarez, C. Ortega-Fuentes, C. Queralto, O. Inostroza, F. Diaz-Yanez, R. Gonzalez, I. L. Calderon, J. A. Fuentes, D. Paredes-Sabja, F. Gil
MICROBIOLOGICAL RESEARCH
(2020)
Article
Microbiology
Lillian G. Acuna, M. Jose Barros, Fernanda Montt, Diego Penaloza, Paula Nunez, Ivan Valdes, Fernando Gil, Juan A. Fuentes, Ivan L. Calderon
Summary: Small noncoding RNAs, such as RyhB, play vital roles in gene expression and physiology regulation in bacteria. In this study, two RyhB homologs, RyhB-1 and RyhB-2, were identified and characterized in the fish pathogen Yersinia ruckeri. Despite similar expression patterns under iron starvation, mutant strains lacking RyhB-1 or RyhB-2 exhibited distinct phenotypes, indicating non-redundant roles in modulating bacterial physiology.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Applied
Alexander Carreno, Dayan Paez-Hernandez, Cesar Zuniga, Angelica Ramirez-Osorio, Nancy Pizarro, Andres Vega, Eduardo Solis-Cespedes, Maria Macarena Rivera-Zaldivar, Andres Silva, Juan A. Fuentes
Summary: Two complexes, fac-Re(CO)(3)(4,5-diazafluoren-9-one)Br (ReL1) and fac-Re(CO)(3)(5,6-epoxy-5,6-dihydro-1,10-phenanthroline)Br (ReL2), were synthesized and characterized in this study, with ReL2 being a novel compound. Structural characterization of both complexes was conducted using various analytical techniques, alongside UV-Vis and luminescence experiments in organic solvents. Relativistic theoretical calculations were also performed to understand electronic transitions and optical properties. Cytotoxicity assessments revealed low or no toxicity for both complexes, with ReL2 showing potential as a luminescent probe for walled cells, such as yeasts and bacteria, observed via confocal microscopy.
Article
Microbiology
Pedro Marchant, Alexander Carreno, Eduardo Vivanco, Andres Silva, Jan Nevermann, Carolina Otero, Eyleen Araya, Fernando Gil, Ivan L. Calderon, Juan A. Fuentes
Summary: While some bacteria's outer membrane vesicles (OMVs) provide resistance to various antimicrobial agents, their role in polymyxin resistance in S. Typhi has not been fully explored.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Alexander Carreno, Manuel Gacitua, Eduardo Solis-Cespedes, Dayan Paez-Hernandez, Wesley B. Swords, Gerald J. Meyer, Marcelo D. Preite, Ivonne Chavez, Andres Vega, Juan A. Fuentes
Summary: SDS-PAGE is used to separate proteins based on size, with anionic dyes being the most commonly used staining method. A newly synthesized monocationic rhenium complex has been found to be a potential luminescent dye for proteins, showing preferential interaction with proteins over the gel matrix. Further studies may explore the use of cationic complexes in combination with traditional anionic compounds for protein staining.
FRONTIERS IN CHEMISTRY
(2021)
Article
Microbiology
Diego Penaloza, Lillian G. Acuna, M. Jose Barros, Paula Nunez, Fernanda Montt, Fernando Gil, Juan A. Fuentes, Ivan L. Calderon
Summary: The sRNAs RyhB-1 and RyhB-2 from S. Typhimurium play vital roles in bacterial infection by regulating intracellular survival, replication, metabolic status, and gene expression associated with Salmonella pathogenicity. Additionally, they directly interact with key bacterial genes, contributing to virulence modulation inside macrophages.
Article
Microbiology
Ivan L. Calderon, Maria Jose Barros, Fernanda Montt, Fernando Gil, Juan A. Fuentes, Lillian G. Acuna
Summary: The RNA chaperone Hfq plays an important role in antibiotic persistence in Yersinia ruckeri, as demonstrated by faster replication and decreased antibiotic tolerance in the hfq-knockout mutant. Hfq is also dependent on the (p)ppGpp synthetase RelA for persister cells production, with the Delta relA and Delta relA Delta hfq strains showing defects in persister cells formation compared to the wild type. The findings shed light on the participation of Hfq in antibiotic persistence in Y. ruckeri.
Article
Chemistry, Applied
Carolina Otero, Sebastian Miranda-Rojas, Felipe M. Llancalahuen, Juan A. Fuentes, Cristian Atala, Gloria Gonzalez-Silva, Diego Verdugo, Paulina Sierra-Rosales, Adrian Moreno, Felipe Gordillo-Fuenzalida
Summary: Peumus boldus is an endemic tree species from Chile, with its leaves and bark being the focus of study for decades. Research has found that extracts contain alkaloids and flavonoids, showing antioxidant and anti-inflammatory activities. In vitro testing of fruit extract demonstrated anti-inflammatory activity, suggesting potential health benefits for the Chilean population.
Review
Food Science & Technology
Maria Carolina Otero, Juan A. Fuentes, Cristian Atala, Sara Cuadros-Orellana, Camila Fuentes, Felipe Gordillo-Fuenzalida
Summary: Food contamination can cause food spoilage and changes in sensory properties. Pathogenic microorganisms in food can lead to serious health problems. Native plants in Chile have antimicrobial properties that can be used in designing new strategies for food pathogen control. Further research is needed to explore their potential effects and applications in active films.
Article
Biochemistry & Molecular Biology
Manuel Gacitua, Alexander Carreno, Rosaly Morales-Guevara, Dayan Paez-Hernandez, Jorge I. Martinez-Araya, Eyleen Araya, Marcelo Preite, Carolina Otero, Maria Macarena Rivera-Zaldivar, Andres Silva, Juan A. Fuentes
Summary: This study explored the antibacterial activities of two Schiff bases derivatives and found that one compound exhibited strong antimicrobial activity against Gram-positive bacteria but was ineffective against Gram-negative bacteria. Furthermore, the compound was comprehensively characterized through various physical and theoretical analyses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Rosaly Morales-Guevara, Juan A. Fuentes, Dayan Paez-Hernandez, Alexander Carreno
Summary: This study focused on luminescent Re(i) tricarbonyl complexes with an asymmetrical pyridine Schiff base (PSB) as ancillary ligand, examining their staining and luminescent properties. Computational studies were performed to determine the complexes' optical properties, revealing that the presence of an intramolecular hydrogen bond (IHB) in the PSB resulted in suitable luminescent emission for biological applications. The choice of substituents at specific positions in the PSB was found to significantly influence the luminescence properties of Re(i) tricarbonyl complexes.
Article
Chemistry, Inorganic & Nuclear
Plinio Cantero-Lopez, Julian Santoyo-Flores, Andres Vega, Alexander Carreno, Juan A. Fuentes, Angelica Ramirez-Osorio, Alejandro Ortiz, Luis Alberto Illicachi, Julio Sanchez, Andres F. Olea, Dayan Paez-Hernandez
Summary: Theoretical methods of the SOC-NEVPT2 type combined with a molecular fragmentation scheme have been used to predict luminescence in a family of compounds, and experimental results confirmed the accuracy of the theoretical predictions.
DALTON TRANSACTIONS
(2021)
Article
Pharmacology & Pharmacy
Mauricio Cabana-Brunod, Pablo A. Herrera, Valeria Marquez-Miranda, Felipe M. Llancalahuen, Yorley Duarte, Danilo Gonzalez-Nilo, Juan A. Fuentes, Cristian Vilos, Luis Velasquez, Carolina Otero
Summary: The study found that iE-DAP encapsulated in PHBV nanoparticles remained stable and efficiently internalized into cells over time. Additionally, the nanoparticles triggered controlled cargo release in vitro, with the encapsulated agonist response higher than its free form, suggesting the potential for activating intracellular receptors and triggering an immune response.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)