Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 34, Issue 6, Pages 283-290Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242422.2016.1265948
Keywords
Biodiesel; Fe3O4 hollow sub-microspheres; immobilized lipase; reusability; waste cooking oil
Funding
- National Natural Science Foundation of China [21406012]
- Fundamental Research Funds for the Central Universities [ZY1417, YS1407]
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Superparamagnetic Fe3O4 hollow sub-microspheres (FHSM) with strong response to an external magnet were prepared via a solvothermal method, followed by acid etching. Lipase from Candida sp. 99-125 was directly immobilized onto the amino-functional FHSM by simple adsorption, without glutaraldehyde linkage. The immobilized lipase was used to catalyze the esterification/transesterification of waste cooking oil with methanol to produce fatty acid methyl ester (FAME), a major source of biodiesel. FAME yield exceeded 93.4% over a wide range of temperatures from 10 to 40 degrees C. Notably, stability was clearly improved at the lower temperatures, in particular, giving a FAME yield of 89.6% after eight cycles of use at 10 degrees C.
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