Article
Obstetrics & Gynecology
Hanna Huebner, Hannah Lubrich, Simon Blum, Sophia Antoniadis, Johannes Lermann, Arif Ekici, Peter A. Fasching, Matthias W. Beckmann, Matthias Ruebner, Stefanie Burghaus
Summary: The study compared two extraction methods for isolating and quantifying ccfDNA from endometriosis patients, with the Maxwell method showing significantly higher yield and the QIAamp method showing higher ccfDNA integrity. Both methods are suitable for downstream applications in non-invasive detection and analysis of somatic mutations within endometriosis tissue.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Genetics & Heredity
Nicholas Kueng, Severine Arcioni, Fanny Sandberg, Christian Kuhn, Vanessa Banz, Carlo R. Largiader, Daniel Sidler, Ursula Amstutz
Summary: Liquid biopsy using quantification of donor-derived cell-free DNA (dd-cfDNA) in plasma has become a novel approach for allograft monitoring in solid organ transplant recipients. This study compared different dd-cfDNA quantification methods and found that the presented high-throughput sequencing method showed strong correlation with existing methods. Absolute levels of dd-cfDNA in urine may be required for allograft surveillance in stable kidney recipients due to extensive variability of relative amounts.
FRONTIERS IN GENETICS
(2023)
Review
Biochemistry & Molecular Biology
Anna Ligasova, Karel Koberna
Summary: Cell quantification is widely used in both basic and applied research, with a special emphasis on techniques based on fluorescent DNA dyes. This review aims to guide readers through the various methods of cell quantification and highlight the strengths and weaknesses of these methods, particularly in terms of sensitivity, accuracy, and time. Additionally, some approaches for analyzing cell proliferation and viability are also described.
Article
Microbiology
Byeong Seob Oh, Won Jung Choi, Ji-Sun Kim, Seoung Woo Ryu, Seung Yeob Yu, Jung-Sook Lee, Seung-Hwan Park, Se Won Kang, Jiyoung Lee, Won Yong Jung, Young-Min Kim, Jae-Ho Jeong, Ju Huck Lee
Summary: This study isolated gut microbiota from healthy individuals and evaluated their anti-colorectal cancer activity in vitro and in vivo. They found that Odoribacter splanchnicus induced anti-proliferative activity through apoptosis, with malic acid identified as a potential active molecule. In a colorectal cancer mouse model, peri-tumoral injection of O. splanchnicus CFS significantly decreased CRC formation.
FRONTIERS IN MICROBIOLOGY
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Peiyong Jiang, Y. M. Dennis Lo
Summary: A new assay for cell-free DNA fragmentation enhances the prediction of gene expression in liquid biopsies.
NATURE BIOTECHNOLOGY
(2022)
Article
Dermatology
Reza Asadzadegan, Negar Haratian, Mahsa Sadeghi, Saman Maroufizadeh, Mohammadreza Mobayen, Hadi Sedigh Ebrahim Saraei, Meysam Hasannejad-Bibalan
Summary: This study investigated the antimicrobial and anti-biofilm effects of indigenous Lactobacillus probiotic strains on Pseudomonas aeruginosa isolated from burn wound infection. The probiotics showed inhibitory effects on pathogenicity factors of P. aeruginosa, including protease, elastase, antibiofilm, and pyocyanin production. Certain strains exhibited a 100% inhibitory effect, while others had a lower effect. The probiotics' cell free supernatant (CFS) significantly decreased the elastolytic activity and protease production of P. aeruginosa, and certain strains exhibited a high inhibitory effect on pyocyanin production and biofilm formation.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Medicine, General & Internal
Eleni Polatoglou, Zsuzsanna Mayer, Vida Ungerer, Abel J. Bronkhorst, Stefan Holdenrieder
Summary: Plasma cell-free DNA (cfDNA) can be extracted from various tissues and cell types and used for non-invasive diagnosis, treatment, and monitoring of cancer and other diseases. This study compared the performance of six commercial cfDNA extraction kits and found significant differences in DNA yield, with the manual QIAamp Circulating Nucleic Acid Kit and automated MagNA Pure Total NA Isolation Kit performing the best. These results highlight the importance of standardizing preanalytical conditions based on downstream applications.
Article
Medicine, General & Internal
Ami Patel, Erika Hissong, Lucelina Rosado, Robert Burkhardt, Lin Cong, Susan A. Alperstein, Momin T. Siddiqui, Hyeon Jin Park, Wei Song, Priya D. Velu, Hanna Rennert, Jonas J. Heymann
Summary: This study characterized the analytical and pre-analytical challenges encountered in the utilization of cfDNA extracted from pleural effusion supernatant in patients with metastatic NSCLC for molecular tumor profiling. Despite some technical challenges, PES was shown to be a robust source of DNA for NGS analysis.
FRONTIERS IN MEDICINE
(2021)
Article
Cell Biology
Jure Krasic, Irena Abramovic, Alen Vrtaric, Nora Nikolac Gabaj, Sasa Kralik-Oguic, Ana Katusic Bojanac, Davor Jezek, Nino Sincic
Summary: Though tissue biopsy has been the standard in biomedicine, personalized medicine is shifting towards liquid biopsies, with cfDNA offering early, non-invasive diagnostics. However, both preanalytical procedures and analytical methods can affect results. Research aims to evaluate factors influencing cfDNA diagnostic parameters in blood and semen, with NucleoSnap identified as the best kit overall.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Yu-Hung Cheng, Chih-Hung Wang, Keng-Fu Hsu, Gwo-Bin Lee
Summary: Ovarian cancer is one of the most severe gynecologic cancers with asymptomatic early stages. This study developed an integrated microfluidic chip that can extract cfDNA from plasma, detect and quantify mutations in the OvCa biomarker BRCA1. By automating the cfDNA extraction and qPCR processes, this chip can be used for clinical screening of OvCa-associated mutations.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Du-Min Jo, Seul-Ki Park, Fazlurrahman Khan, Min-Gyun Kang, Jae-Hwa Lee, Young-Mog Kim
Summary: The study shows that treating ribbonfish with LAB cell-free culture can improve food quality, extend shelf life, delay microbial growth, and reduce trimethylamine content. This method has the potential to prolong the shelf life of raw fish fillets and maintain their quality during storage.
Article
Biology
Yaojia Chen, Yuxin Gong, Lijun Dou, Xun Zhou, Ying Zhang
Summary: This review discusses the contributions of bioinformatics tools to the study of cfDNA, focusing on cfDNA identification signals, identification methods, and its relationship with human diseases such as hepatic cancer, lung cancer, end-stage kidney disease, and ischemic stroke. The research significance and existing problems of using cfDNA as a biomarker for diseases are also discussed.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Gokhan Kursad Incili, Muzeyyen Akgol, Pinar Karatepe, Sefa Uner, Ali Tekin, Hilal Kanmaz, Busra Kaya, Mehmet Calicioglu, Ali Adnan Hayaloglu
Summary: The specific aims of this study were to determine and quantify the bioactive compounds in the cell-free supernatant (CFS) of Pediococcus acidilactici and test their protective effect in frankfurters using an edible coating. The results showed that the CFS contained a wide variety of bioactive compounds, and immersing frankfurters in CFS and chitosan solution extended their shelf life by inhibiting oxidation and microbial spoilage.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Medicine, Research & Experimental
Rossella Salemi, Silvia Vivarelli, Daria Ricci, Marina Scillato, Maria Santagati, Giuseppe Gattuso, Luca Falzone, Massimo Libra
Summary: This study found that LGG-SN has anti-proliferative activity against colon cancer and metastatic melanoma cells and enhances the efficacy of anti-cancer drugs. This suggests that LGG could be a new therapeutic approach in oncology to reduce chemotherapy-related side effects and overcome resistance issues, improving the treatment response in cancer patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Microbiology
Sol Lee, Han-Oh Park, Wonbeak Yoo
Summary: This study investigates the effects of cell-free supernatant from Lactobacillus gasseri BNR17 on anti-melanogenesis and reducing oxidative stress in both murine melanoma cells and human keratinocytes. The results show that the cell-free supernatant can effectively inhibit melanin production and has antioxidative effects.
