Article
Biochemistry & Molecular Biology
Abdelnaby Khalyfa, Juan F. Masa, Zhuanhong Qiao, Monica Gonzalez, Sergi Marti, Ahamed A. Khalyfa, Leila Kheirandish-Gozal, David Gozal
Summary: Exosomes from OHS patients enhance the aggressiveness of lung cancer cells and this effect can be partially reversed by CPAP treatment.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Nekane Romero-Garcia, Javier Huete-Acevedo, Cristina Mas-Bargues, Jorge Sanz-Ros, Mar Dromant, Consuelo Borras
Summary: The exponential growth in the elderly population and their associated socioeconomic burden have recently brought aging research into the spotlight. To integrate current knowledge and guide potential interventions, nine biochemical pathways are summarized under the term hallmarks of aging. These hallmarks are deeply inter-related and act together to drive the aging process. Altered intercellular communication is particularly relevant since it explains how damage at the cellular level translates into age-related loss of function at the organismal level. As the main effectors of intercellular communication, extracellular vesicles (EVs) might play a key role in the aggravation or mitigation of the hallmarks of aging. This review aims to summarize this role and provide context for the multiple emerging EV-based gerotherapeutic strategies that are currently under study.
Article
Cell Biology
Tom G. Keulers, Sten F. Libregts, Joel E. J. Beaumont, Kim G. Savelkouls, Johan Bussink, Hans Duimel, Ludwig Dubois, Marijke Zonneveld, Carmen Lopez-Iglesias, Karel Bezstarosti, Jeroen A. Demmers, Marc Vooijs, Marca Wauben, Kasper M. A. Rouschop
Summary: GABARAPL1 is essential for cargo loading and secretion of extracellular vesicles, with GABARAPL1(+)EVs being detectable and targetable for blocking pro-angiogenic effects. Silencing GABARAPL1 results in impaired early endosomal pathway and secretion of EVs with pro-angiogenic properties, affecting tumor growth and therapy efficacy.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Medicine, General & Internal
Abdelnaby Khalyfa, Aaron Ericsson, Zhuanghong Qiao, Isaac Almendros, Ramon Farre, David Gozal
Summary: Experiments on mice mimicking obstructive sleep apnea revealed that intermittent hypoxia exposure leads to changes in gut microbiota, increased gut permeability, altered plasma exosome cargo, and adipocyte dysfunction, ultimately resulting in metabolic dysfunction. Additionally, physical activity can improve these alterations.
Article
Oncology
Mona Alharbi, Andrew Lai, Shayna Sharma, Priyakshi Kalita-de Croft, Nihar Godbole, America Campos, Dominic Guanzon, Alexis Salas-Burgos, Flavio Carrion, Felipe A. Zuniga, Lewis Perrin, Yaowu He, Tanja Pejovic, Carmen Winters, Terry Morgan, John D. Hooper, Gregory E. Rice, Carlos Salomon
Summary: Ovarian cancer is one of the most lethal cancers worldwide affecting women, with non-specific early symptoms. sEVs produced under low oxygen tension can induce a metabolic switch in ovarian cancer cells, promoting resistance to carboplatin. The presence of glycolysis pathway proteins in sEVs is closely associated with ovarian cancer recurrence.
Article
Cell Biology
Ryan Wallis, Natasa Josipovic, Hannah Mizen, Arturo Robles-Tenorio, Eleanor J. Tyler, Argyris Papantonis, Cleo L. Bishop
Summary: The secretion of small extracellular vesicles (EVs) by senescent cells is found to play an important role in aging and age-related pathologies. Separating the soluble SASP from the vesicular component helps clarify the functional significance of EVs and provides a framework for future investigations.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Cell Biology
Wenyi Zheng, Rui He, Xiuming Liang, Samantha Roudi, Jeremy Bost, Coly Pierre-Michael, Guillaume van Niel, Samir E. L. Andaloussi
Summary: This study presents a reconfigurable glycoengineering strategy to produce extracellular vesicles (EVs) with strong cellular specificity by endogenously displaying glycans of interest on the EV surface. The strategy showcases the surface display of different glycan ligands on EVs and achieves high specificity towards specific cell types, leading to enhanced therapeutic effects and attenuation of cell damage.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Abdelnaby Khalyfa, Jose M. Marin, David Sanz-Rubio, Zhen Lyu, Trupti Joshi, David Gozal
Summary: This study investigated the changes in plasma-derived exosomes in patients with obstructive sleep apnea (OSA) before and after continuous positive airway pressure (CPAP) treatment, and identified potential disease biomarkers. The results showed that CPAP treatment improved endothelial cell function and tube formation in OSA patients. Through multi-omics analysis, the researchers also identified differentially expressed proteins, lipids, and miRNAs associated with OSA. These findings contribute to a better understanding of the pathogenesis of OSA and provide new directions for its treatment and management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Alicja Gluszko, Miroslaw J. Szczepanski, Theresa L. Whiteside, Torsten E. Reichert, Jacek Siewiera, Nils Ludwig
Summary: Tissue hypoxia is a common feature in HNSCC, leading to alterations in tumor cells. The study found that sEVs released by HNSCC cells under hypoxic conditions have a unique protein composition, which can potentially serve as biomarkers for different degrees of tissue hypoxia and as a resource for anti-angiogenic therapies.
Article
Geriatrics & Gerontology
Xiaogang Chu, Kumar Subramani, Bobby Thomas, Alvin V. Terry, Sadanand Fulzele, Raghavan Pillai Raju
Summary: Studies have shown that factors in the blood of young organisms can rejuvenate the old ones. In this study, it was discovered that treating aged mice with plasma-derived factors from young mice can prolong their lives and reduce oxidative stress. The treatment also modulates the Nrf2/Bach1 axis in the antioxidant response pathway, promoting injury resolution.
Article
Biochemistry & Molecular Biology
David Sanz-Rubio, Abdelnaby Khalyfa, Zhuanhong Qiao, Jorge Ullate, Jose M. Marin, Leila Kheirandish-Gozal, David Gozal
Summary: The study found that intermittent hypoxia affects the release of EVs from different types of cells, with some EVs potentially leading to endothelial dysfunction. These findings suggest that cell-derived EVs may play important roles in some adverse outcomes associated with obstructive sleep apnea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Article
Biochemistry & Molecular Biology
Yoon Young Go, Chan Mi Lee, Won Min Ju, Sung-Won Chae, Jae-Jun Song
Summary: Research has shown that trophoblast-derived secretomes significantly promote the migration and proliferation of skin cells, leading to a delay in dermal aging through protection and restoration processes. Additionally, there is an increase in gene expression of dermal extracellular matrix components, indicating the potential for skin regeneration and anti-aging effects mediated by trophoblast-derived secretomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Giorgia Manni, Sandra Buratta, Maria Teresa Pallotta, Davide Chiasserini, Alessandro Di Michele, Carla Emiliani, Stefano Giovagnoli, Luisa Pascucci, Rita Romani, Ilaria Bellezza, Lorena Urbanelli, Francesca Fallarino
Summary: Extracellular vesicles (EVs) are membrane-enclosed particles secreted by cells and circulating in body fluids. They not only serve as a tool for waste disposal, but also transmit cell signals. Aging is characterized by genomic instability, telomere erosion, epigenetic alteration, and defective proteostasis. Studies have shown that EVs released in cell models are associated with these aging hallmarks and can spread senescence signals. Furthermore, EVs have great potential as anti-aging tools. Although this is a recent field, altered EV secretion in aging suggests it as an emerging hallmark of aging.
Article
Cell Biology
Akaitz Dorronsoro, Fernando E. Santiago, Diego Grassi, Tianpeng Zhang, Ruenn Chai Lai, Sara J. McGowan, Luise Angelini, Mitra Lavasani, Lana Corbo, Aiping Lu, Robert W. Brooks, Marta Garcia-Contreras, Donna B. Stolz, Antonio Amelio, Siddaraju V. Boregowda, Mohammad Fallahi, Adrian Reich, Camillo Ricordi, Donald G. Phinney, Johnny Huard, Sai Kiang Lim, Laura J. Niedernhofer, Paul D. Robbins
Summary: The injection of BM-MSCs from young mice can prolong lifespan and healthspan, while conditioned media (CM) from young BM-MSCs can rescue the function of aged stem cells and senescent fibroblasts. Extracellular vesicles (EVs) from young BM-MSC CM can extend lifespan in mice, and EVs from MSCs generated from human ES cells can reduce senescence and improve healthspan.
Article
Cell Biology
Jonas Carmona-Pirez, Beatriz Poblador-Plou, Jesus Diez-Manglano, Manuel Jesus Morillo-Jimenez, Jose Maria Marin Trigo, Ignatios Ioakeim-Skoufa, Antonio Gimeno-Miguel, Alexandra Prados-Torres
Summary: By using network analysis to compare the multimorbidity patterns of HF and COPD in men and women, we identified specific diseases highly connected in all networks and detected common clusters as well as group-specific patterns, providing insights for further study and clinical implications. Network analysis serves as a powerful tool to analyze and visualize the complex relationships in multimorbidity patterns of COPD and HF.
MECHANISMS OF AGEING AND DEVELOPMENT
(2021)
Review
Biochemistry & Molecular Biology
Abdelnaby Khalyfa, David Sanz-Rubio
Summary: Sleep is crucial for overall health and quality of life, but sleep disorders can lead to the development of various diseases, including neurodegenerative diseases. Obstructive sleep apnea (OSA) is a common sleep disorder that may cause decreased blood oxygenation, disrupted sleep cycles, and the generation of free radicals impacting nitric oxide bioavailability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Respiratory System
Rene Cortese, David Sanz-Rubio, Leila Kheirandish-Gozal, Jose Maria Marin, David Gozal
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Clinical Neurology
Juan F. Masa, Ivan D. Benitez, Shahrokh Javaheri, Maria Victoria Mogollon, Maria A. Sanchez-Quiroga, Francisco J. Gomez de Terreros, Jaime Corral, Rocio Gallego, Auxiliadora Romero, Candela Caballero-Eraso, Estrella Ordax-Carbajo, Maria F. Troncoso, Monica Gonzalez, Soledad Lopez-Martin, Jose M. Marin, Sergi Marti, Trinidad Diaz-Cambriles, Eusebi Chiner, Carlos Egea, Javier Barca, Ferran Barbe, Babak Mokhlesi
Summary: This study aims to evaluate the risk factors involved in the pathogenesis of pulmonary hypertension (PH) in untreated obesity hypoventilation syndrome (OHS) patients. The results indicate that obesity per se is a major independent risk factor for PH, and weight loss interventions may have a critical role in improving PH in this patient population.
JOURNAL OF CLINICAL SLEEP MEDICINE
(2022)
Article
Respiratory System
Ana Ezponda, Ciro Casanova, Miguel Divo, Marta Marin-Oto, Carlos Cabrera, Jose M. Marin, Gorka Bastarrika, Victor Pinto-Plata, Angela Martin-Palmero, Francesca Polverino, Bartolome R. Celli, Juan P. de Torres
Summary: This study found that conducting chest CT scans on COPD patients can help diagnose related comorbidities, and identified some comorbidities associated with all-cause mortality, most of which are difficult to detect clinically.
Article
Respiratory System
Alberto Alonso-Fernandez, Maria Cerda Moncadas, Ainhoa Alvarez Ruiz De Larrinaga, Andres Sanchez Baron, Mercedes Codina Marcet, Paula Rodriguez Rodriguez, Ana Victoria Gil Gomez, Maria P. Gimenez Carrero, Carla Pia Martinez, Jose Pablo Cubero Marin, Monica de la Pena, Antonia Barcelo, Daniel Morell-Garcia, Javier Pierola, Jose Antonio Pena Zarza, Joaquin Duran Cantolla, Jose Maria Marin Trigo, Joan B. Soriano, Francisco Garcia-Rio
Summary: This study aimed to evaluate the prevalence of obstructive sleep apnea (OSA) in pregnant women and its association with gestational diabetes mellitus (GDM), as well as the relationship between sleep parameters and insulin resistance (IR). The results showed no significant difference in OSA prevalence during the third trimester of pregnancy between GDM patients and non-GDM controls, and no associations between OSA and GDM determinants were found. Multiple regression analysis revealed that total sleep time, time spent with oxygen saturation< 90%, and maximum duration of respiratory events were independently associated with IR.
ARCHIVOS DE BRONCONEUMOLOGIA
(2022)
Editorial Material
Respiratory System
Francesca Polverino, Jose M. Marin
Summary: Persistent airflow limitation in children and young adults is a term that encompasses various biological and clinical manifestations starting before birth and throughout the lifespan. Events occurring during the first years of life play a key role in predicting lung function trend. Asthma and lower respiratory tract infections are major factors that contribute to airflow limitation, but the association between early-life respiratory infections and the risk of wheezing or asthma in later life remains unknown.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Endocrinology & Metabolism
J. Lazaro, D. Sanz-Rubio, P. Claveria, C. Cabrejas, J. Fernando, A. Castanera, J. M. Marin
Summary: Obstructive sleep apnea and severe obesity alter the profile of extracellular vesicle miRNAs. Bariatric surgery and weight loss do not reverse this effect, while CPAP treatment shows a recovery outcome in extracellular vesicle miRNAs in patients with severe obesity and OSA.
INTERNATIONAL JOURNAL OF OBESITY
(2023)
Article
Medicine, General & Internal
Alvise Casara, Graziella Turato, Marta Marin-Oto, Umberto Semenzato, Davide Biondini, Mariaenrica Tine, Nicol Bernardinello, Elisabetta Cocconcelli, Pablo Cubero, Elisabetta Balestro, Paolo Spagnolo, Jose M. Marin, Manuel G. Cosio, Marina Saetta, Erica Bazzan
Summary: Chronic bronchitis (CB) significantly affects symptoms, quality of life, and survival in smokers without COPD, with 27% of noCOPD smokers having CB. CB does not impact FEV1 decline in noCOPD but does significantly in COPD patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Editorial Material
Critical Care Medicine
Joan B. Soriano, Jose M. Marin, Bartolome R. Celli
LANCET RESPIRATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Elena Diaz-Garcia, Aldara Garcia-Sanchez, David Sanz-Rubio, Enrique Alfaro, Cristina Lopez-Fernandez, Raquel Casitas, Eva Manas Baena, Irene Cano-Pumarega, Pablo Cubero, Marta Marin-Oto, Eduardo Lopez-Collazo, Jose Maria Marin, Francisco Garcia-Rio, Carolina Cubillos-Zapata
Summary: Patients with obstructive sleep apnea (OSA) are more likely to develop atherosclerosis, leading to cardiovascular diseases. The transforming growth factor (TGF-beta) is crucial in atherosclerosis development, and SMAD4 is the central regulator of TGF-beta pathway. This study analyzed SMAD4 levels in OSA patients and found elevated levels in both plasma and monocytes, especially in patients with early subclinical atherosclerosis (eSA). The study also revealed that intermittent hypoxia and NLRP3 play a role in SMAD4 upregulation and release. This research underscores the potential of sSMAD4 as a biomarker for atherosclerosis risk in OSA patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Abdelnaby Khalyfa, Jose M. Marin, David Sanz-Rubio, Zhen Lyu, Trupti Joshi, David Gozal
Summary: This study investigated the changes in plasma-derived exosomes in patients with obstructive sleep apnea (OSA) before and after continuous positive airway pressure (CPAP) treatment, and identified potential disease biomarkers. The results showed that CPAP treatment improved endothelial cell function and tube formation in OSA patients. Through multi-omics analysis, the researchers also identified differentially expressed proteins, lipids, and miRNAs associated with OSA. These findings contribute to a better understanding of the pathogenesis of OSA and provide new directions for its treatment and management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Respiratory System
Marta Marin-Oto, David Sanz-Rubio, Fernando Santamaria-Martos, Ivan Benitez, Ana L. Simon, Marta Forner, Pablo Cubero, Ana Gil, Manuel Sanchez-de-laTorre, Ferran Barbe, Jose M. Marin
Summary: The study found that patients with hypoxia-related diseases (including COPD, OSA, and OSA-COPD overlap) had lower levels of sRAGE compared to healthy individuals. However, patients with OSA who received CPAP treatment, especially those with concurrent COPD, showed a significant increase in sRAGE levels.
RESPIRATORY RESEARCH
(2022)