Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 293, Issue -, Pages 304-311Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.05.013
Keywords
Electrochemiluminescence; Self-enhancement; Coreaction accelerator; Strand displacement reaction; Helicobacter pylori
Funding
- National Natural Science Foundation of China [81873980, 21804015, 81672080]
- Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0206]
- Youth Project of Chongqing Municipal Education Commission Science and Technology [KJQN201800440]
- China Postdoctoral Science Foundation [2017M622979]
- Chongqing Special Postdoctoral Science Foundation [XmT2018073]
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Herein, a novel ternary electrochemiluminescence (ECL) nanosphere containing the emitter of luminol (Lum), the coreactant of polyethylenimine (PEI) and the coreaction accelerator of amino-terminated perylene derivative (PTC-NH2) was prepared for the first time, which possessed strong ECL emission due to intramolecular coreaction of self-enhanced PEI-Lum and intermolecular coreaction acceleration of PTC-NH2 to Lum/H2O2 system. Combining the Lum-based ternary ECL nanosphere with target-triggered strand displacement reaction, a highly sensitive and enzyme-free biosensor was constructed for determination of Helicobacter pylori DNA. This developed ECL biosensing method exhibited a wide linear range from 10 fM to 10 nM and a detection limit down to 2.4 fM for Helicobacter pylori DNA detection. Therefore, this work provides a new avenue to develop high-performance ECL nanomaterials and an enzyme-free bioassay to be applied in the clinical molecular diagnosis of gastric diseases.
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