Article
Immunology
Duo Zhang, Min Zheng, Ying Zhang, Guanrong Feng, Chengcheng Peng, Chenghui Li, Yiquan Li, He Zhang, Nan Li, Pengpeng Xiao
Summary: Metavirome sequencing and PCR analysis of pangolin samples from Guangxi Province, China revealed the presence of various viral sequences, including Japanese encephalitis virus, Getah virus, and chikungunya virus. Additionally, sudden and recurrent viruses were observed, prompting future dissemination tests.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Virology
Yuan Fang, Emad I. M. Khater, Jing-Bo Xue, Enas H. S. Ghallab, Yuan-Yuan Li, Tian-Ge Jiang, Shi-Zhu Li
Summary: This systematic review examines the prevalence of mosquito-borne viruses (MBVs) in Egypt. The study finds that dengue virus (DENV) and West Nile virus (WNV) have shown an increase in seropositivity rates, and spillover events have been recorded. The surveillance system for MBVs in Egypt has deficiencies and calls for the establishment of an early warning system and a joint system for medical care and public health.
Review
Pharmacology & Pharmacy
Ivo C. C. Martins, Rafaela C. C. Ricardo, Nuno C. C. Santos
Summary: Dengue, West Nile, and Zika viruses are vector-borne flaviviruses that cause disease outbreaks with potentially severe symptoms and socioeconomic repercussions. Currently, there are no specific treatments available, and only generic symptom relief is possible. The effectiveness of the first dengue vaccine, Dengvaxia, is limited. Prophylactic approaches against other viruses are even more restricted. Therefore, there is a need for therapeutic strategies.
Article
Immunology
Chengcheng Peng, Duo Zhang, Chenghui Li, Yiquan Li, He Zhang, Nan Li, Pengpeng Xiao
Summary: This study gathered Rhinolophus sinicus samples and used metavirome techniques to obtain enriched viral sequences of the Rhinolophus sinicus-specific virome. Chikungunya virus (CHIKV) and Japanese encephalitis virus (JEV) present in Rhinolophus sinicus were validated through PCR. Notably, it was the first time JEV was isolated from Rhinolophus sinicus. The newly isolated JEV sequence showed increased infectivity during passaging in Vero cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Lukas Wilkman, Clas Ahlm, Magnus Evander, Olivia Wesula Lwande
Summary: Five endemic mosquito-borne viruses in Fennoscandia have significant impact on human health, but their incidence remains unknown due to underdiagnosing and lack of surveillance. The complex transmission and lack of clear case definition criteria make prevention and diagnosis of Fennoscandian moboviruses challenging, leading to potential misdiagnosis. Some infections have long-term effects such as malaise, rash, and joint pain. Research on mobovirus diseases is mainly focused on a few pathogens, neglecting many others. This review aims to summarize current medically relevant knowledge of moboviruses in Fennoscandia while highlighting unknown and overlooked aspects.
FRONTIERS IN MEDICINE
(2023)
Article
Parasitology
Cecilia S. S. Engdahl, Chinmay V. V. Tikhe, George Dimopoulos
Summary: Vector control plays a key role in reducing the burden of mosquito-borne diseases. Synthetic insecticides used in current strategies can have negative environmental impact and become ineffective due to mosquito resistance. This study presents the discovery and evaluation of natural product-based biological control agents as potential biopesticides for mosquito control. Several natural products were identified as capable of killing mosquito larvae, including those responsible for transmitting diseases such as dengue, Zika, chikungunya, and yellow fever. The study also explores the usefulness of crude extracts from two bacterial sources for mosquitocidal activity.
PARASITES & VECTORS
(2022)
Review
Entomology
Zhao-Yang Wang, Kai-Xiao Nie, Ji-Chen Niu, Gong Cheng
Summary: Mosquito-borne viruses (MBVs) pose a significant threat to global public health, including dengue virus, Zika virus, Japanese encephalitis virus, West Nile virus, and chikungunya virus. Mosquito saliva contains proteins that not only facilitate blood feeding but also play important roles in regulating local and systemic infection, as well as modulating the host immune responses. This review discusses the physiological functions of mosquito salivary proteins (MSPs), their impact on MBV transmission, and the current progress and challenges in developing MSP-based MBV transmission blocking vaccines.
Article
Virology
Megan A. Wallace, Kelsey A. Coffman, Clement Gilbert, Sanjana Ravindran, Gregory F. Albery, Jessica Abbott, Eliza Argyridou, Paola Bellosta, Andrea J. Betancourt, Herve Colinet, Katarina Eric, Amanda Glaser-Schmitt, Sonja Grath, Mihailo Jelic, Maaria Kankare, Iryna Kozeretska, Volker Loeschcke, Catherine Montchamp-Moreau, Lino Ometto, Banu Sebnem Onder, Dorcas J. Orengo, John Parsch, Marta Pascual, Aleksandra Patenkovic, Eva Puerma, Michael G. Ritchie, Omar Rota-Stabelli, Mads Fristrup Schou, Svitlana Serga, Marina Stamenkovic-Radak, Marija Tanaskovic, Marija Savic Veselinovic, Jorge Vieira, Cristina P. Vieira, Martin Kapun, Thomas Flatt, Josefa Gonzalez, Fabian Staubach, Darren J. Obbard
Summary: A study identified multiple DNA viruses in Drosophila in Europe, including new nudiviruses, entomopoxvirus, and viruses related to other insects. The prevalence of viruses in European Drosophila reflects that seen in publicly available datasets, with DNA viruses showing lower prevalence in Drosophila compared to RNA viruses.
Article
Virology
Megan A. Wallace, Kelsey A. Coffman, Clement Gilbert, Sanjana Ravindran, Gregory F. Albery, Jessica Abbott, Eliza Argyridou, Paola Bellosta, Andrea J. Betancourt, Herve Colinet, Katarina Eric, Amanda Glaser-Schmitt, Sonja Grath, Mihailo Jelic, Maaria Kankare, Iryna Kozeretska, Volker Loeschcke, Catherine Montchamp-Moreau, Lino Ometto, Banu Sebnem Onder, Dorcas J. Orengo, John Parsch, Marta Pascual, Aleksandra Patenkovic, Eva Puerma, Michael G. Ritchie, Omar Rota-Stabelli, Mads Fristrup Schou, Svitlana Serga, Marina Stamenkovic-Radak, Marija Tanaskovic, Marija Savic Veselinovic, Jorge Vieira, Cristina P. Vieira, Martin Kapun, Thomas Flatt, Josefa Gonzalez, Fabian Staubach, Darren J. Obbard
Summary: The study identified 14 DNA viruses from Drosophila collected in Europe, including three new nudiviruses and a virus related to entomopoxvirus. Drosophila Kallithea nudivirus and Drosophila Vesanto virus were found to be relatively common, while the other viruses were rare. This suggests that DNA viruses are less prevalent in Drosophila compared to RNA viruses and may not persist as well in laboratory cultures.
Article
Biochemical Research Methods
Fahmida Nasrin, Indra Memdi Khoris, Ankan Dutta Chowdhury, Sjakurrizal El Muttaqein, Enoch Y. Park
Summary: To prevent viral diseases, the development of a disposable, rapid, and convenient biosensor with high sensitivity and reliability is highly desired. In this study, a practical impedimetric biosensor has been created on a disposable electrode for the detection of two mosquito-borne viruses. The biosensor fabrication involves the formation of a conductive film, physical binding of gold nanoparticles-polyaniline, and biofunctionalization. The disposable electrode has been designed to detect Chikungunya virus and Zika virus separately with a wide linear range and low detection limits.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Microbiology
Matheus Augusto Calvano Cosentino, Mirela D'arc, Filipe Romero Rebello Moreira, Liliane Tavares de Faria Cavalcante, Ricardo Mouta, Amanda Coimbra, Francine Bittencourt Schiffler, Thamiris dos Santos Miranda, Gabriel Medeiros, Cecilia A. Dias, Antonizete R. Souza, Maria Clotilde Henriques Tavares, Amilcar Tanuri, Marcelo Alves Soares, Andre Felipe Andrade dos Santos
Summary: This study detected and characterized novel anelloviruses in the common black-tufted marmoset through high-throughput sequencing, expanding the host range of Anelloviridae and providing important insights into viral evolution dynamics.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Virology
Eric Agboli, Julien B. Z. Zahouli, Athanase Badolo, Hanna Joest
Summary: Mosquito-associated viruses, especially arboviruses like dengue, yellow fever, chikungunya, and Zika, pose a significant threat to human and animal health in West Africa. There is a lack of in-depth knowledge about the ecology of these viruses and related mosquitoes in the region. Enhancing understanding of mosquito-associated viruses and their impact on host mosquito ecology is crucial for improving arbovirus prevention and control strategies in West Africa.
Article
Multidisciplinary Sciences
Takayuki Amemiya, Katsuhisa Horimoto, Kazuhiko Fukui
Summary: The study focused on applying computational drug repositioning to five mosquito-borne viral infections, identifying 77 drug candidates and 146 proteins for these diseases. Signature molecules and pathways for each virus infection were determined based on omics analyses, and drugs were classified according to the degree of target proteins in the protein-protein interaction network.
SCIENTIFIC REPORTS
(2021)
Article
Parasitology
Daniel Chieh-Ding Lin, Shih-Che Weng, Po-Nien Tsao, Justin Jang Hann Chu, Shin-Hong Shiao
Summary: Co-infection of dengue virus and Zika virus in Aedes aegypti mosquitoes enhance viral replication and suggest that the NS5 protein is a potential target for vector control.
PARASITES & VECTORS
(2023)
Article
Microbiology
Lu Tan, Yiwen Zhang, Dal Young Kim, Runsheng Li
Summary: Mosquito-borne alphaviruses pose a significant threat to human and animal health, but specific antivirals or vaccines are currently unavailable. Eilat virus (EILV) and its chimeras have been developed as potential biological control measures against these viruses. This study investigated the interactions between these insect-specific viruses (ISVs) and mosquito cells, revealing their roles in vector competence and arbovirus transmission. The chimeras induced stronger antiviral responses in host cells compared to the parental EILV, and initial chimera infections inhibited subsequent pathogenic alphavirus replication. These findings highlight the potential of insect-specific chimeras as promising candidates for developing biological control measures against pathogenic alphaviruses.
MICROBIOLOGY SPECTRUM
(2023)
Article
Environmental Sciences
Lu Zhao, Evans Atoni, Raphael Nyaruaba, Yao Du, Huaiyu Zhang, Oscar Donde, Doudou Huang, Shuqi Xiao, Nanjie Ren, Teng Ma, Zhu Shu, Zhiming Yuan, Lei Tong, Han Xia
Summary: Wastewater-based epidemiology (WBE) is an effective environmental surveillance tool for monitoring fecal-oral pathogen infections within a community. This study focused on detecting SARS-CoV-2 RNA in various samples from wastewater systems and related environments in Wuhan during the COVID-19 middle and low risk periods. The findings demonstrated the significance of WBE in continuous surveillance of SARS-CoV-2, even when the COVID-19 prevalence is low.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Virology
Yi Huang, Hongqing Zhang, Xiaodan Li, Lu Zhao, Dirui Cai, Shunlong Wang, Nanjie Ren, Haixia Ma, Doudou Huang, Fei Wang, Zhiming Yuan, Bo Zhang, Han Xia
Summary: This study detected a strain of mosquito flavivirus (MFV) in Culicoides collected from Yunnan, China, and successfully rescued the virus using an infectious clone. Further analysis of the biological features of the virus in vitro and in vivo showed strong replication ability in cells but low infection rate in mosquitoes.
Article
Infectious Diseases
Cihan Yang, Fei Wang, Doudou Huang, Haixia Ma, Lu Zhao, Guilin Zhang, Hailong Li, Qian Han, Dennis Bente, Ferdinand Villanueva Salazar, Zhiming Yuan, Han Xiai
Summary: The global impact of mosquito-borne diseases has increased in recent decades. This study found that Aedes aegypti mosquitoes have the potential to spread Ebinur Lake virus (EBIV) through biting, with a transmission rate of up to 11.8%. The study also revealed a complex interplay between EBIV and the mosquito's immune system, which could affect its vector competence.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Letter
Cell Biology
Dali Tong, Mei Zhang, Yunru Yang, Han Xia, Haiyang Tong, Huajun Zhang, Weihong Zeng, Muziying Liu, Yan Wu, Huan Ma, Xue Hu, Weiyong Liu, Yuan Cai, Yanfeng Yao, Yichuan Yao, Kunpeng Liu, Shifang Shan, Yajuan Li, Ge Gao, Weiwei Guo, Yun Peng, Shaohong Chen, Juhong Rao, Jiaxuan Zhao, Juan Min, Qingjun Zhu, Yanmin Zheng, Lianxin Liu, Chao Shan, Kai Zhong, Zilong Qiu, Tengchuan Jin, Sandra Chiu, Zhiming Yuan, Tian Xue
Article
Infectious Diseases
Yanshuang Wang, Xuemiao Li, David A B Dance, Han Xia, Chen Chen, Nini Luo, Anyang Li, Yanmei Li, Qiao Zhu, Qinghui Sun, Xingyong Wu, Yingfei Zeng, Lin Chen, Shen Tian, Qianfeng Xia
Summary: The study evaluated the clinical potential of Burkholderia pseudomallei phages isolated from Hainan, China, for treating melioidosis. Results showed that the phage exhibited promising lytic properties and stability, significantly improving cell and nematode survival rates.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Multidisciplinary Sciences
Fei Wang, Cihan Yang, Shunlong Wang, Qun Wu, Christabel Ochieng, Zhiming Yuan, Han Xia
Summary: Mosquito-borne viruses pose a severe threat to public health as they are transmitted by many mosquito species. This study presents a method for detecting viruses in the salivary glands of Aedes aegypti, which can help assess the risk of these viruses spread by mosquitoes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Infectious Diseases
Jinxin Meng, Fei Wang, Yuwen He, Nan Li, Zhenxing Yang, Jun Yao, Shunlong Wang, Guodian Xiong, Zhiming Yuan, Han Xia, Jinglin Wang
Summary: A novel Oya virus (OYAV) isolate SZC50 was sequenced from midge samples and its host tropism was tested in vitro, showing high susceptibility in pig-derived cell lines. High prevalence of OYAV SZC50 neutralization antibody was found in Yunnan pig serum samples. Pathogenicity of OYAV SZC50 was demonstrated in animal models, with adult and suckling mice being extremely susceptible and chicken embryos showing a fatality rate of 37.5%.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Multidisciplinary Sciences
Ying Huang, Shunlong Wang, Hong Liu, Evans Atoni, Fei Wang, Wei Chen, Zhaolin Li, Sergio Rodriguez, Zhiming Yuan, Zhaoyan Ming, Han Xia
Summary: This study created a comprehensive dataset of arboviruses and ASVs, revealing the diversity, distribution, and biosafety recommendations of these viruses globally. It provides insights into potential interactions, evolution, and risks associated with arboviruses and ASVs.
Article
Biochemistry & Molecular Biology
Guoguo Ye, Zhixiang Xu, Minghui Yang, Jun Wang, Jinhu Liang, Juzhen Yin, Yang Yang, Han Xia, Yingxia Liu
Summary: Based on the surveillance of dengue virus (DENV) infections in Shenzhen, it was found that the majority of DENV-infected patients are young to middle-aged males, and the disease is accompanied by abnormal changes in blood cell percentages. The concentration of these patients in Futian District may be due to higher mosquito density and temperature. Mosquito infection experiments confirmed the effect of temperature on DENV proliferation and transmission, with constant temperatures enhancing the spread of the virus and increasing the risk of epidemics.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Biochemical Research Methods
Zhaoyan Ming, Xiangjun Chen, Shunlong Wang, Hong Liu, Zhiming Yuan, Minghui Wu, Han Xia
Summary: The article presents HostNet, a deep learning framework for predicting virus hosts from genomic sequences. HostNet utilizes a Transformer-CNN-BiGRU architecture and two enhanced sequence representation modules to overcome the challenges of data deficiency and imbalance. The results show that HostNet outperforms the state-of-the-art deep learning-based method in host prediction accuracy and F1 score. The improved sequence representation modules significantly enhance HostNet's training generalization, performance in challenging classes, and stability.
BMC BIOINFORMATICS
(2023)
Letter
Virology
Mengying Liang, Wanbo Tai, Yunyun Wang, Yulong Dai, Yanfeng Yao, Juan Min, Yiwu Zhou, Liang Liu, Chao Shan, Han Xia, Zhiming Yuan
Letter
Virology
Ge Gao, Xue Hu, Yiwu Zhou, Juhong Rao, Xiaoyu Zhang, Yun Peng, Jiaxuan Zhao, Yanfeng Yao, Kunpeng Liu, Mengying Liang, Hang Liu, Fei Deng, Han Xia, Chao Shan, Zhiming Yuan