Article
Medicine, Research & Experimental
Laura Pascual-Reguant, Queralt Serra-Camprubi, Debayan Datta, Damiano Cianferoni, Savvas Kourtis, Antoni Ganez-Zapater, Chiara Cannata, Lorena Espinar, Jessica Querol, Laura Garcia-Lopez, Sara Musa-Afaneh, Maria Guirola, Anestis Gkanogiannis, Andrea Miro Canturri, Marta Guzman, Olga Rodriguez, Andrea Herencia-Ropero, Joaquin Arribas, Violeta Serra, Luis Serrano, Tian Tian, Sandra Peiro, Sara Sdelci
Summary: The simultaneous inhibition of LOXL2 and BRD4 has been found to effectively suppress the proliferation of triple-negative breast cancer (TNBC), offering a potential new strategy for its treatment. By disrupting the interactions between LOXL2 and BRD4, the transcriptional regulation of cell cycle genes is affected, leading to the suppression of TNBC cell proliferation.
EMBO MOLECULAR MEDICINE
(2023)
Article
Genetics & Heredity
Justine Marsolier, Pacome Prompsy, Adeline Durand, Anne-Marie Lyne, Camille Landragin, Amandine Trouchet, Sabrina Tenreira Bento, Almut Eisele, Sophie Foulon, Lea Baudre, Kevin Grosselin, Mylene Bohec, Sylvain Baulande, Ahmed Dahmani, Laura Sourd, Eric Letouze, Anne-Vincent Salomon, Elisabetta Marangoni, Leila Perie, Celine Vallot
Summary: The persistence of drug-resistant cancer cells is a major clinical challenge, particularly in triple-negative breast cancer. This study reveals that the repressive histone mark H3K27me3 plays a crucial role in regulating cell fate and chemotherapy tolerance in cancer cells. Manipulating H3K27me3 levels effectively enhances the potential of cancer cells to tolerate chemotherapy and delays tumor recurrence. These findings underscore the importance of understanding chromatin landscapes in shaping cancer cell response to initial therapy.
Article
Multidisciplinary Sciences
Wanfu Wu, Margaret Warner, Li Wang, Wei-Wei He, Ruipeng Zhao, Xiaoxiang Guan, Cindy Botero, Bo Huang, Charlotte Ion, Charles Coombes, Jan-Ake Gustafsson
Summary: Comparing gene expression profiles of malignant parts and normal adjacent parts of TNBC breasts revealed that CYP-mediated pathways may drive TNBC. However, despite being expressed in TNBC, ER13 is unlikely to be a tumor suppressor as its absence of main tethering partners renders it ineffective on genes related to proliferation and inflammation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Debo Chen, Zhishan Chen, Zhitang Wang, Chengye Hong, Qinglan Wang, Peidong Yang, Zhidong Huang, Weibin Lian, Yiqin Huang, Wei Fu, Junjing Li, Zhipeng Hong
Summary: This research identified a lncRNA called SEMA3B-AS1 that is downregulated in triple negative breast cancer (TNBC) and is associated with unfavorable prognosis. Functional experiments showed that SEMA3B-AS1 inhibits tumor-initiating cell behaviors and suppresses tumor formation, migration, and invasion. Mechanistic insights revealed that SEMA3B-AS1 interacts with MLL4 to activate the expression of SEMA3B, a known tumor suppressor gene. This study enhances our understanding of the role of lncRNAs in TNBC and suggests their potential as therapeutic targets.
MOLECULAR CARCINOGENESIS
(2023)
Article
Biochemistry & Molecular Biology
Sandra Torres-Ruiz, Eduardo Tormo, Iris Garrido-Cano, Ana Lameirinhas, Federico Rojo, Juan Madoz-Gurpide, Octavio Burgues, Cristina Hernando, Begona Bermejo, Maria Teresa Martinez, Ana Lluch, Juan Miguel Cejalvo, Pilar Eroles
Summary: Due to the lack of specific targets, cytotoxic chemotherapy remains the standard treatment for triple-negative breast cancer. However, chemotherapy not only affects tumor cells but also modulates the tumor microenvironment in a way that favors tumor propagation. In this study, we found that the expression of the lymphangiogenic receptor VEGFR3 was higher in doxorubicin-resistant cells compared to parental cells. VEGFR3 silencing reduced cell proliferation and migration in both cell lines, and high VEGFR3 expression was associated with worse survival in chemotherapy-treated patients. Our results suggest that targeting VEGFR3 in combination with chemotherapy could be a potential therapeutic strategy for triple-negative breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Juliana Haydee Enrique Steinberg, Fabiana Alejandra Rossi, Roberto Magliozzi, Laurensia Yuniati, Matteo Santucci, Mario Rossi, Daniele Guardavaccaro, Angela Lauriola
Summary: The research demonstrates that SHARP1 functions as a suppressor of metastasis in TNBC. It inhibits the invasive phenotype of TNBC by blocking hypoxia-inducible factors. Additionally, targeting the beta TrCP-dependent degradation of SHARP1 shows potential as a therapeutic strategy in TNBC.
CELL DEATH & DISEASE
(2023)
Article
Biology
Kanishk Jain, Matthew R. Marunde, Jonathan M. Burg, Susan L. Gloor, Faith M. Joseph, Karl F. Poncha, Zachary B. Gillespie, Keli L. Rodriguez, Irina K. Popova, Nathan W. Hall, Anup Vaidya, Sarah A. Howard, Hailey F. Taylor, Laylo Mukhsinova, Ugochi C. Onuoha, Emily F. Patteson, Spencer W. Cooke, Bethany C. Taylor, Ellen N. Weinzapfel, Marcus A. Cheek, Matthew J. Meiners, Geoffrey C. Fox, Kevin E. W. Namitz, Martis W. Cowles, Krzysztof Krajewski, Zu-Wen Sun, Michael S. Cosgrove, Nicolas L. Young, Michael-Christopher Keogh, Brian D. Strahl
Summary: This study reveals that H3 tail acetylation promotes nucleosomal accessibility to H3K4 methyl readers and writers, resolving the long-standing question of why H3K4me3 levels are coupled with H3 acetylation.
Article
Medicine, Research & Experimental
Cuiyu Zhong, Jie Li, Suiping Liu, Weirong Li, Qiang Zhang, Junpeng Zhao, Menghua Xiong, Yan Bao, Yandan Yao
Summary: Researchers developed a nanoblock-mediated strategy to selectively induce anticancer activity of broad-spectrum oncolytic peptides (Olps) for the treatment of triple-negative breast cancer (TNBC). The results showed that the strategy significantly reduced the toxicity of Olps and demonstrated high antitumor efficacy in TNBC cells.
Article
Biotechnology & Applied Microbiology
Liyang Cui, Arvin M. Gouw, Edward L. LaGory, Shenghao Guo, Nabeel Attarwala, Yao Tang, Ji Qi, Yun-Sheng Chen, Zhou Gao, Kerriann M. Casey, Arkadiy A. Bazhin, Min Chen, Leeann Hu, Jinghang Xie, Mingxi Fang, Cissy Zhang, Qihua Zhu, Zhiyuan Wang, Amato J. Giaccia, Sanjiv Sam Gambhir, Weiping Zhu, Dean W. Felsher, Mark D. Pegram, Elena A. Goun, Anne Le, Jianghong Rao
Summary: The research developed a mitochondria-targeted copper-depleting nanoparticle that effectively inhibited the growth of triple-negative breast cancer cells and improved survival rates in mouse models. By depriving copper within cancer cells, this approach induced metabolic changes and apoptosis, highlighting its potential clinical relevance.
NATURE BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Arushi Jaiswal, Kiichi Murakami, Andrew Elia, Yukiko Shibahara, Susan J. Done, Stephen A. Wood, Nicholas J. Donato, Pamela S. Ohashi, Michael Reedijk
Summary: The study suggests that inhibition of USP9x can treat TNBC by reducing Notch activity, decreasing tumor inflammation, enhancing antitumor immune response, and suppressing tumor growth. Pharmacological inhibition of USP9x can remodel the tumor immune landscape, reduce tumor growth without toxicity, and target Notch in metabolically vulnerable tissues like TNBC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Wei Wang, Dong Han, Qinbo Cai, Tao Shen, Bingning Dong, Michael T. Lewis, Runsheng Wang, Yanling Meng, Wolong Zhou, Ping Yi, Chad J. Creighton, David D. Moore, Feng Yang
Summary: This study demonstrates that in a subset of TNBC, MAPK4 activation can occur independently of PI3K and promote tumor growth, and that inhibiting MAPK4 can increase the sensitivity of these tumors to PI3K blockade.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Xiangwei Ge, Deyu Zhang, Songze Song, Yue Mi, Yanjie Shen, Qiwei Jiang, Yingchun Liang, Jinliang Wang, Qinong Ye
Summary: USP18 expression is increased in paclitaxol-resistant TNBC cells. Overexpression of USP18 reduces paclitaxol sensitivity and induces autophagy, leading to chemotherapy resistance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Mengna Zhang, Lingxian Zhang, Ajun Geng, Xiao Li, Yu Zhou, Liming Xu, Yi Arial Zeng, Jinpeng Li, Cheguo Cai
Summary: In this study, the researchers investigated the function of cyclin-dependent kinase 14 (CDK14) in mammary development and breast cancer progression. The results showed that CDK14 is expressed in mammary basal cells and elevated in triple negative breast cancer (TNBC). CDK14 knockdown or inhibition was found to suppress mammary regeneration and the progression of TNBC.
Letter
Medicine, General & Internal
Ryan Sun, Lee-Jen Wei
Summary: This article discusses the clinical benefits of pembrolizumab combined with chemotherapy in patients with triple-negative breast cancer. The authors suggest that both hazard values and ratios should be considered when evaluating clinical benefits.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Oncology
Ingrid Espinoza, Chandra Kurapaty, Cheol-Hong Park, Travis Vander Steen, Celina G. Kleer, Elizabeth Wiley, Alfred Rademaker, Elisabet Cuyas, Sara Verdura, Maria Buxo, Carol Reynolds, Javier A. Menendez, Ruth Lupu
Summary: Triple-negative/basal-like breast cancer is characterized by aggressive biological features. Targeting CCN1/CYR61 may have therapeutic value in suppressing the biological aggressiveness of this type of breast cancer.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Marco Di Marsico, Andreu Paytuvi Gallart, Walter Sanseverino, Riccardo Aiese Cigliano
Summary: The GreeNC database is a key reference database for studying plant lncRNAs, with the most recent update adding annotation for a large number of species and applying sequence clustering to provide additional information. The current version of the database can be accessed at the provided link.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Anna Adam-Artigues, Enrique J. Arenas, Alex Martinez-Sabadell, Fara Braso-Maristany, Raimundo Cervera, Eduardo Tormo, Cristina Hernando, Maria Teresa Martinez, Juan Carbonell-Asins, Soraya Simon, Jesus Poveda, Santiago Moragon, Sandra Zazo, Debora Martinez, Ana Rovira, Octavio Burgues, Federico Rojo, Joan Albanell, Begona Bermejo, Ana Lluch, Aleix Prat, Joaquin Arribas, Pilar Eroles, Juan Miguel Cejalvo
Summary: AXL overexpression is identified as an essential mechanism of trastuzumab resistance in HER2-positive breast cancer, and targeting AXL can restore trastuzumab response. AXL may serve as a predictive biomarker for prognosis in HER2-positive breast cancer patients. The study emphasizes the importance of targeting AXL in combination with anti-HER2 drugs to overcome resistance.
Article
Veterinary Sciences
Leyna Diaz, Gemma Castella, M. Rosa Bragulat, Andreu Paytuvi-Gallart, Walter Sanseverino, F. Javier Cabanes
Summary: This study assessed and quantified the presence of Malassezia yeast in canine pododermatitis using various methods, and found that the amount of Malassezia yeast was higher in samples before treatment.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Manuel Gamez-Chiachio, Angela Molina-Crespo, Carmen Ramos-Nebot, Jeannette Martinez-Val, Lidia Martinez, Katja Gassner, Francisco J. Llobet, Mario Soriano, Alberto Hernandez, Marco Cordani, Cristina Bernado-Morales, Eva Diaz, Alejandro Rojo-Sebastian, Juan Carlos Trivino, Laura Sanchez, Ruth Rodriguez-Barrueco, Joaquin Arribas, David Llobet-Navas, David Sarrio, Gema Moreno-Bueno
Summary: The over-expression of GSDMB is associated with poor prognosis and aggressive behavior in HER2 breast cancer. This study reveals a functional link between GSDMB over-expression and protective autophagy in response to HER2-targeted therapies. GSDMB acts as an autophagy adaptor and plays a crucial role in modulating autophagosome maturation through Rab7 activation. These findings provide potential new targeted treatments for HER2/GSDMB aggressive tumors.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Surgery
Anna Buxeda, Laura Llinas-Mallol, Javier Gimeno, Dolores Redondo-Pachon, Carlos Arias-Cabrales, Carla Burballa, Adrian Puche, Miguel Lopez-Botet, Jose Yelamos, Carlos Vilches, Maarten Naesens, Maria Jose Perez-Saez, Julio Pascual, Marta Crespo
Summary: Isolated microvascular inflammation (iMVI) is a rare phenotype that cannot be classified as antibody-mediated rejection (ABMR) in recent Banff classifications because it lacks HLA donor-specific antibodies or C4d deposition. In this study, we compared peripheral blood leukocyte distribution and inflammatory infiltrates in kidney transplant biopsies among ABMR, iMVI, and normal cases. We found that iMVI had fewer lymphocytes and peripheral T cells compared to ABMR and normal cases, but more NKG2A+ NK cells. iMVI also showed decreased plasma cell infiltration and increased cytotoxic T cell infiltration compared to ABMR.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Oncology
Queralt Serra-Camprubi, Helena Verdaguer, Winona Oliveros, Nuria Lupion-Garcia, Alba Llop-Guevara, Cristina Molina, Maria Vila-Casadesus, Anthony Turpin, Cindy Neuzillet, Joan Frigola, Jessica Querol, Mariana Yanez-Bartolome, Florian Castet, Carles Fabregat-Franco, Carmen Escudero-Iriarte, Marta Escorihuela, Enrique J. Arenas, Cristina Bernado-Morales, Noemi Haro, Francis J. Giles, Oscar J. Pozo, Josep M. Miquel, Paolo G. Nuciforo, Ana Vivancos, Marta Mele, Violeta Serra, Joaquim Arribas, Josep Tabernero, Sandra Peiro, Teresa Macarulla, Tian Tian
Summary: It has been found that advanced cholangiocarcinoma patients with BRCA2 gene mutations are likely to benefit from PARP inhibitor therapy, while those with mutations in IDH1, ARID1A, or BAP1 will not. The patient-derived xenograft models provide new opportunities for evaluating drug response and prioritizing clinical trials.
CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Maria Perez-Peiro, Paula Valenti-Serra, Blanca Leon-Gonzalez, Coral Ampurdanes, Xavier Duran, Jose Yelamos, Esther Barreiro
Summary: In this study, it was found that the PARP inhibitor rucaparib reduced tumor burden in lung adenocarcinoma in mice through multiple biological mechanisms, including apoptosis and oxidative stress. Treatment with rucaparib resulted in decreased PARP activity and cell proliferation, increased DNA damage and apoptosis, and elevated levels of protein oxidation and SOD2. These findings suggest the potential of PARP inhibitors for reducing tumor burden in lung adenocarcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Amandine Moretton, Savvas Kourtis, Antoni Ganez Zapater, Chiara Calabro, Maria Lorena Espinar Calvo, Frederic Fontaine, Evangelia Darai, Etna Abad Cortel, Samuel Block, Laura Pascual-Reguant, Natalia Pardo-Lorente, Ritobrata Ghose, Matthew G. Vander Heiden, Ana Janic, Andre C. Mueller, Joanna Loizou, Sara Sdelci
Summary: This study investigates the role of cellular metabolism in DNA damage repair and identifies Peroxiredoxin 1 (PRDX1) as a contributor to this process. By integrating functional genomics with chromatin proteomics and metabolomics, the interplay between cellular metabolism and the DNA damage response is elucidated.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Genetics & Heredity
Lise Mangiante, Nicolas Alcala, Alexandra Sexton-Oates, Alex Di Genova, Abel Gonzalez-Perez, Azhar Khandekar, Erik N. Bergstrom, Jaehee Kim, Xiran Liu, Ricardo Blazquez-Encinas, Colin Giacobi, Nolwenn Le Stang, Sandrine Boyault, Cyrille Cuenin, Severine Tabone-Eglinger, Francesca Damiola, Catherine Voegele, Maude Ardin, Marie-Cecile Michallet, Lorraine Soudade, Tiffany M. Delhomme, Arnaud Poret, Marie Brevet, Marie-Christine Copin, Sophie Giusiano-Courcambeck, Diane Damotte, Cecile Girard, Veronique Hofman, Paul Hofman, Jerome Mouroux, Charlotte Cohen, Stephanie Lacomme, Julien Mazieres, Vincent Thomas de Montpreville, Corinne Perrin, Gaetane Planchard, Nathalie Rousseau, Isabelle Rouquette, Christine Sagan, Arnaud Scherpereel, Francoise Thivolet, Jean-Michel Vignaud, Didier Jean, Anabelle Gilg Soit Ilg, Robert Olaso, Vincent Meyer, Anne Boland-Auge, Jean-Francois Deleuze, Janine Altmuller, Peter Nuernberg, Alejandro Ibanez-Costa, Justo P. Castano, Sylvie Lantuejoul, Akram Ghantous, Charles Maussion, Pierre Courtiol, Hector Hernandez-Vargas, Christophe Caux, Nicolas Girard, Nuria Lopez-Bigas, Ludmil B. Alexandrov, Francoise Galateau-Salle, Matthieu Foll, Lynnette Fernandez-Cuesta
Summary: By integrating large-scale whole-genome sequencing data, transcriptomic data, and epigenomic data, researchers found that the current World Health Organization classification only explains 10% of the molecular differences between patients. Instead, the MESOMICS project establishes a morphomolecular classification of malignant pleural mesothelioma based on four dimensions: ploidy, tumor cell morphology, adaptive immune response, and CpG island methylator profile. These findings reveal the interplay between MPM functional biology and its genomic history and provide new insights into the variations observed in the clinical behavior of MPM patients.
Article
Biochemistry & Molecular Biology
George Rasti, Maximilian Becker, Berta N. Vazquez, Maria Espinosa-Alcantud, Irene Fernandez-Duran, Andres Gamez-Garcia, Alessandro Ianni, Jessica Gonzalez, Laia Bosch-Presegue, Anna Marazuela-Duque, Anna Guitart-Solanes, Sandra Segura-Bayona, Joan-Josep Bech-Serra, Michael Scher, Lourdes Serrano, Uma Shankavaram, Hediye Erdjument-Bromage, Paul Tempst, Danny Reinberg, Mireia Olivella, Travis H. Stracker, Carolina de la Torre, Alejandro Vaquero
Summary: SIRT1 plays a crucial role in regulating the DNA damage response by interacting with the PP4 phosphatase complex to control the signaling of DNA damage and repair.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Genetics & Heredity
Santiago A. Gonzalez, Nuria J. Lopez-Bigas, Abel A. Gonzalez-Perez
Summary: Chemotherapies can cause DNA damage and specific single nucleotide variants. This study found that platinum-based chemotherapies can also lead to larger mutational footprints, characterized by an increase in chromosomal fragments of copy number. These structural variants induced by platinum-based chemotherapies may have implications for tumor evolution and long-term side effects.
Review
Oncology
Santiago Duro-Sanchez, Macarena Roman Alonso, Joaquin Arribas
Summary: A variety of treatments are available for HER2-positive breast cancer, but resistance to these therapies is common and associated with poor prognosis. Immunotherapeutic approaches are being explored to eradicate tumor cells and prevent relapse and progression. This review discusses the different immunotherapeutic strategies being tested and their potential benefits for HER2-positive breast cancer.
Article
Biochemistry & Molecular Biology
Giovanni Sarnelli, Alessandro Del Re, Marcella Pesce, Jie Lu, Giovanni Esposito, Walter Sanseverino, Chiara Corpetti, Silvia Basili Franzin, Luisa Seguella, Irene Palenca, Sara Rurgo, Fatima Domenica Elisa De Palma, Aurora Zilli, Giuseppe Esposito
Summary: Researchers proposed an engineered Escherichia coli strain with SARS-CoV-2 spike protein to elicit potent mucosal immunity. The intragastrically administered, engineered EcN demonstrated the ability to produce specific systemic and mucosal immunoglobulins against SARS-CoV-2 spike protein in mice, indicating its potential as an oral vaccine against COVID-19. The vaccine is safe, inexpensive, and capable of stimulating the production of both systemic and mucosal anti-SARS-CoV-2 spike protein antibodies.
Article
Plant Sciences
Simone Landi, Paola Punzo, Roberta Nurcato, Rossella Albrizio, Walter Sanseverino, Riccardo Aiese Cigliano, Pasquale Giorio, Florinda Fratianni, Giorgia Batelli, Sergio Esposito, Stefania Grillo
Summary: 'Corbarino' (COR) and 'Lucariello' (LUC) are Mediterranean long shelf-life tomato landraces that produce high-quality fruits under low water input cultivation. Understanding their unique drought stress tolerance at morpho-physiological and molecular levels may be crucial for their utilization in breeding programs.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
