Article
Multidisciplinary Sciences
Elise Y. Cai, Jose Garcia, Yuzhen Liu, Funda Vakar-Lopez, Sonali Arora, Holly M. Nguyen, Bryce Lakely, Lisha Brown, Alicia Wong, Bruce Montgomery, John K. Lee, Eva Corey, Jonathan L. Wright, Andrew C. Hsieh, Hung-Ming Lam
Summary: The study established an aggressive and cisplatin-resistant bladder cancer patient-derived xenograft (PDX) and organoid system, showing a progression towards an increasingly proliferative and stem-like expression profile. Both models maintained histological fidelity and mutational heterogeneity of the parental tumor.
SCIENTIFIC REPORTS
(2021)
Article
Clinical Neurology
Lisa Kristina Isbell, Cordula Tschuch, Soroush Doostkam, Silvia Waldeck, Geoffroy Andrieux, Khalid Shoumariyeh, Dorothee Lenhard, Hans Eckart Schaefer, Peter Christoph Reinacher, Ingrid Bartsch, Milena Pantic, Janaki Manoja Vinnakota, Vinodh Kakkassery, Elisabeth Schorb, Florian Scherer, Anna Verena Frey, Melanie Boerries, Gerald Illerhaus, Justus Duyster, Julia Schueler, Nikolas von Bubnoff
Summary: This study established a patient-derived central nervous system lymphoma xenograft mouse model, which successfully mimicked the pathology of central nervous system lymphoma and primary vitreoretinal lymphoma. Transcriptomic analysis revealed distinct gene regulation patterns between these two types of lymphoma. This model provides a valuable tool for studying the mechanisms of lymphoma cell tropism to the central nervous system and identifying potential therapeutic targets.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2023)
Review
Medicine, Research & Experimental
Shahrokh Abdolahi, Zeinab Ghazvinian, Samad Muhammadnejad, Mahshid Saleh, Hamid Asadzadeh Aghdaei, Kaveh Baghaei
Summary: The establishment and application of patient-derived xenograft models have played a significant role in cancer treatment. These models accurately represent the biological characteristics of different cancers and have great value in the development of anti-cancer drugs and personalized medicine.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Loraine Jarrosson, Clelia Costechareyre, Fanny Gallix, Severine Cire, Fabien Gay, Olivier Imbaud, Romain Teinturier, Elisabetta Marangoni, Karine Aguera, Celine Delloye-Bourgeois, Valerie Castellani
Summary: The lack of preclinical patient-derived xenograft cancer models for large-scale molecular studies hampers the development of effective personalized therapies. This study introduces an in vivo concept of implanting human tumor cells in avian embryos for targeted therapeutics delivery and evaluating efficacy through light sheet confocal microscopy. It demonstrates the feasibility of this model for characterizing therapeutic-induced changes in gene expression related to triple-negative breast cancer.
Article
Engineering, Biomedical
Yanhong Duo, Daoming Zhu, Xiurong Sun, Meng Suo, Zheng Zheng, Wei Jiang, Ben Zhong Tang
Summary: A novel AIEgen-based SDT system was developed, with DCPy showing advantages over traditional sonosensitizers. Patient-derived MV/AIEgen hybrids were used for personalized SDT, demonstrating superior tumor targeting ability and efficient therapy.
Review
Pathology
Domenico Ribatti
Summary: The chick embryo chorioallantoic membrane (CAM) is a fusion of the chorion with the vascularized allantoic membrane, performing multiple functions during embryonic development. It is a widely used model for studying angiogenesis, tumor growth, and drug efficacy. This review article focuses on the effects of patient-derived xenografts (PDX) on the CAM, highlighting the use of CAM for pharmacological screenings and immune response-free transplantation.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Oncology
T. Magnes, S. Wagner, A. R. Thorner, D. Neureiter, E. Klieser, G. Rinnerthaler, L. Weiss, F. Huemer, N. Zaborsky, M. Steiner, S. Weis, R. Greil, A. Egle, T. Melchardt
Summary: Genetic comparison between primary DLBCL and SCNSL revealed substantial clonal diversification at relapse in some patients, highlighting a potential mechanism of treatment resistance.
Article
Multidisciplinary Sciences
Ben Yi Tew, Bodour Salhia
Summary: PDX models are beneficial in studying CNS metastasis, with various implantation techniques providing insights into different stages of metastasis. The choice of implantation method should be based on the specific scientific question and study goals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Food Science & Technology
Siro Luvisetto
Summary: Botulinum neurotoxins are powerful inhibitors of synaptic vesicle fusion and neurotransmitter release, with effects not only at the peripheral neuromuscular junction but also potentially on the central nervous system. They are highly effective in treating muscle hyperactivity disorders and may have therapeutic applications for conditions of the CNS.
Article
Biology
Dennis Ma, Grace A. Hernandez, Austin E. Y. T. Lefebvre, Hamad Alshetaiwi, Kerrigan Blake, Kushal R. Dave, Maha Rauf, Justice W. Williams, Ryan T. Davis, Katrina T. Evans, Aaron Longworth, Madona Y. G. Masoud, Regis Lee, Robert A. Edwards, Michelle A. Digman, Kai Kessenbrock, Devon A. Lawson
Summary: A 3D culture-transplant system was developed for studying cancer metastasis using patient-derived xenograft (PDX) tumor cells, showing that OXPHOS inhibition attenuates lung metastatic capacity of breast cancer cells and that overexpression of the metabolic enzyme NME1 increases lung metastasis. The system allows for lentiviral engineering and dissemination of tumor cells, validating its authenticity for investigations of metastasis and revealing a new role for NME1 in promoting breast cancer metastasis.
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Gloria Echeverria, Shirong Cai, Yizheng Tu, Jiansu Shao, Emily Powell, Abena B. Redwood, Yan Jiang, Aaron McCoy, Amanda L. Rinkenbaugh, Rosanna Lau, Alexander J. Trevarton, Chunxiao Fu, Rebekah Gould, Elizabeth E. Ravenberg, Lei Huo, Rosalind Candelaria, Lumarie Santiago, Beatriz E. Adrada, Deanna L. Lane, Gaiane M. Rauch, Wei T. Yang, Jason B. White, Jeffrey T. Chang, Stacy L. Moulder, W. Fraser Symmans, Susan G. Hilsenbeck, Helen Piwnica-Worms
Summary: This study established 369 orthotopic PDX models of breast cancer and found that treatment-resistant cancers were more likely to be successfully engrafted. The molecular subtype of breast cancer was not associated with engraftment rate. A statistical model predictive of engraftment was developed based on clinical indicators. These breast cancer models are valuable for clinical treatment and biomarker research.
Article
Cell Biology
Hui Yao, Jiangang Liu, Chi Zhang, Yunxiang Shao, Xuetao Li, Zhengquan Yu, Yulun Huang
Summary: Our study revealed that apatinib inhibits the malignancy of glioma cells by targeting THBS1, potentially through MYH9. This could provide novel targets for glioma therapy.
CELL DEATH & DISEASE
(2021)
Review
Oncology
Binhua Pan, Xuyong Wei, Xiao Xu
Summary: Animal models are essential for evaluating human cancers and investigating anticancer drugs and prevention. In hepatopancreatobiliary cancer research, mouse models are widely used. While current CDX models have limitations, the emerging PDX model shows promise for precision cancer medicine in hepatopancreatobiliary cancer research.
CANCER CELL INTERNATIONAL
(2022)
Article
Oncology
Angela Mastronuzzi, Giovanna Stefania Colafati, Andrea Carai, Maria D'Egidio, Francesco Fabozzi, Francesca Del Bufalo, Maria Felicia Villani, Giada Del Baldo, Sabina Vennarini, Costanza Canino, Angela Di Giannatale, Paolo Toma, Maria Carmen Garganese, Maria Antonietta De Ioris
Summary: Central nervous system (CNS) metastatic spread in neuroblastoma (NB) is rare and occurs more often at relapse/progression. This retrospective study found that CNS metastasis in NB is confirmed to be rare, occurring in 4.7% of patients, and associated with advanced disease and bone skull involvement. The study also observed a higher incidence of CNS involvement at relapse in the immunotherapy era. Further research is needed to confirm the increased risk of CNS relapse in the immunotherapy era and the necessity of including CNS imaging in follow-up.
Article
Oncology
Mingchen Shi, Yu Wang, Dong Lin, Yuzhuo Wang
Summary: Patient-derived xenografts (PDXs) have gained significant attention in cancer research as relevant models due to their retention of histopathological and molecular heterogeneity from donor tumors. Neuroendocrine prostate cancer (NEPC) PDX models are increasingly being generated and have shown utility in various research applications. This review provides a comprehensive overview of NEPC PDX collections, highlighting their potential for identifying therapeutic targets and evaluating drug responses.
Article
Genetics & Heredity
Jamie L. Grit, Benjamin K. Johnson, Patrick S. Dischinger, Curt J. Essenburg, Marie Adams, Stacy Campbell, Kai Pollard, Christine A. Pratilas, Tim J. Triche, Carrie R. Graveel, Matthew R. Steensma
Summary: This study confirms that CNFs and PNFs are epigenetically distinct tumors with differences in higher-order chromatin states and specific methylation events altering genes involved in key biological and cellular processes. The findings demonstrate differential RAS/MKK3/p38 signaling between CNFs and PNFs, with CNFs showing pro-inflammatory signaling and chromatin conformational changes, whereas PNFs signaling predominantly through RAS/MEK. Tumor size also correlated with specific CpG methylation events.
EPIGENETICS & CHROMATIN
(2021)
Article
Otorhinolaryngology
Linda X. Yin, Emily E. Karp, Anna Elias, Thomas J. O'Byrne, David M. Routman, Daniel L. Price, Jan L. Kasperbauer, Michelle Neben-Wittich, Ashish V. Chintakuntlawar, Katharine A. Price, Daniel J. Ma, Robert L. Foote, Eric J. Moore, Kathryn M. Van Abel
Summary: Delayed diagnosis of HPV(+)OPSCC is associated with a greater burden of disease at presentation, but oncologic outcomes remain favorable across treatment modalities.
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2021)
Article
Oncology
Michael P. Grams, Dawn Owen, Sean S. Park, Ivy A. Petersen, Michael G. Haddock, Elizabeth B. Jeans, Randi R. Finley, Daniel J. Ma
Summary: A novel and practical VMAT planning approach for grid therapy is described in this study, which can be efficiently delivered in a clinically practical time span and has demonstrated effective treatment outcomes for tumor patients.
PRACTICAL RADIATION ONCOLOGY
(2021)
Article
Hematology
Allison J. Kaeding, Sonali P. Barwe, Anilkumar Gopalakrishnapillai, Rhonda E. Ries, Todd A. Alonzo, Robert B. Gerbing, Colin Correnti, Michael R. Loken, Lisa Eidenschink Broderson, Laura Pardo, Quy H. Le, Thao Tang, Amanda R. Leonti, Jenny L. Smith, Cassie K. Chou, Min Xu, Tim Triche, Steven M. Kornblau, E. Anders Kolb, Katherine Tarlock, Soheil Meshinchi
Summary: The study identified mesothelin (MSLN) as highly overexpressed in a significant proportion of acute myeloid leukemia (AML) patients, associated with certain gene rearrangements and expression patterns, and suggested that MSLN may serve as a diagnostic and therapeutic target in AML. Additionally, MSLN-directed therapeutic strategies, including antibody-drug conjugates (ADCs), showed promising cytotoxic effects in MSLN+ AML cells.
Article
Public, Environmental & Occupational Health
Alexis C. Dennis, Esther O. Chung, Evans K. Lodge, Rae Anne Martinez, Rachel E. Wilbur
Summary: The article highlights the significant impact of structural racism on health inequalities in the United States, presenting a new framework to illustrate the unequal effects of structural racism on health opportunities among different racial and ethnic groups.
ETHNICITY & DISEASE
(2021)
Editorial Material
Oncology
Michelle Mierzwa, Beth M. Beadle, Melvin L. K. Chua, Daniel J. Ma, David J. Thomson, Danielle N. Margalit
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Medicine, Research & Experimental
Ryan A. McMillan, Kathryn M. Van Abel, Linda X. Yin, David M. Routman, Daniel J. Ma, Michelle A. Neben Wittich, Daniel L. Price, Jan L. Kasperbauer, Katharine R. Price, Ashish V. Chintakuntlawar, Eric J. Moore
Summary: The study compared rates of metachronous and synchronous second primaries of the contralateral tonsil in patients with primary HPV(+) tonsillar squamous cell carcinoma. The prevalence of metachronous second primaries after appropriate treatment was very low (0.3%), as was the chance of incidentally discovering a synchronous second primary during bilateral tonsillectomy (2.7%). It was concluded that bilateral tonsillectomy should not be routine in the surgical management of these patients.
Article
Otorhinolaryngology
Elizabeth B. Jeans, Satomi Shiraishi, Gohar Manzar, Lindsay K. Morris, Adam Amundson, Lisa A. McGee, Jean-Claude Rwigema, Michelle Neben-Wittich, David M. Routman, Daniel J. Ma, Samir H. Patel, Robert L. Foote, Scott C. Lester
Summary: IMPT is associated with decreased dose to OARs and less patient-reported acute deterioration in multiple head and neck domains. Physician-reported toxicities demonstrated less oral pain. Strong consideration for IMPT in ipsilateral head and neck patients with cancer is warranted.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2022)
Article
Biochemical Research Methods
Benjamin D. Lee, Anthony Gitter, Casey S. Greene, Sebastian Raschka, Finlay Maguire, Alexander J. Titus, Michael D. Kessler, Alexandra J. Lee, Marc G. Chevrette, Paul Allen Stewart, Thiago Britto-Borges, Evan M. Cofer, Kun-Hsing Yu, Juan Jose Carmona, Elana J. Fertig, Alexandr A. Kalinin, Brandon Signal, Benjamin J. Lengerich, Timothy J. Triche Jr, Simina M. Boca
PLOS COMPUTATIONAL BIOLOGY
(2022)
Review
Public, Environmental & Occupational Health
Rae Anne M. Martinez, Nafeesa Andrabi, Andrea N. Goodwin, Rachel E. Wilbur, Natalie R. Smith, Paul N. Zivich
Summary: Despite scholars' calls for critical engagement with race and ethnicity concepts in US epidemiologic research, the inclusion of these social constructs in scholarship has been inadequate. This study examines how race and ethnicity are conceptualized, operationalized, and utilized in US research published in leading epidemiology journals. A systematic review of 1,050 articles revealed that the practices have remained largely unchanged between 1995 and 2018, indicating the need for improvement.
AMERICAN JOURNAL OF EPIDEMIOLOGY
(2023)
Article
Multidisciplinary Sciences
Benjamin J. Huang, Jenny L. Smith, Jason E. Farrar, Yi-Cheng Wang, Masayuki Umeda, Rhonda E. Ries, Amanda R. Leonti, Erin Crowgey, Scott N. Furlan, Katherine Tarlock, Marcos Armendariz, Yanling Liu, Timothy Shaw, Lisa Wei, Robert B. Gerbing, Todd M. Cooper, Alan S. Gamis, Richard Aplenc, E. Anders Kolb, Jeffrey Rubnitz, Jing Ma, Jeffery M. Klco, Xiaotu Ma, Todd A. Alonzo, Timothy Triche, Soheil Meshinchi
Summary: The study established a robust transcriptional risk prediction model for pediatric AML, finding that LSC47 is more predictive than LSC17 and increases the predictive power of conventional risk stratification. Applying biomarkers informed by cytomolecular profiling outperforms a uniform approach.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Oncology
Lauren M. Harmon, Timothy J. Triche Jr
Summary: Genomic monitoring is superior to cytogenetics for clinical assessment of leukemia. A case of pediatric acute lymphoblastic leukemia demonstrates that a lesion underlying lethal end-stage myeloid disease could be detected by whole-genome sequencing years before it manifested cytogenetically.
Article
Biochemical Research Methods
Samantha L. Wilson, Shu Yi Shen, Lauren Harmon, Justin M. Burgener, Tim Triche, Scott Bratman, Daniel D. De Carvalho, Michael M. Hoffman
Summary: This study developed a method called cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) and designed a set of synthetic spike-in DNA controls for quantitative normalization.
CELL REPORTS METHODS
(2022)
Article
Oncology
Nadeem Riaz, Eric Sherman, Xin Pei, Heiko Schoder, Milan Grkovski, Ramesh Paudyal, Nora Katabi, Pier Selenica, Takafumi N. Yamaguchi, Daniel Ma, Simon K. Lee, Rachna Shah, Rahul Kumar, Fengshen Kuo, Abhirami Ratnakumar, Nathan Aleynick, David Brown, Zhigang Zhang, Vaios Hatzoglou, Lydia Y. Liu, Adriana Salcedo, Chiaojung J. Tsai, Sean McBride, Luc G. T. Morris, Jay Boyle, Bhuvanesh Singh, Daniel S. Higginson, Rama R. Damerla, Arnaud da Cruz Paula, Katharine Price, Eric J. Moore, Joaquin J. Garcia, Robert Foote, Alan Ho, Richard J. Wong, Timothy A. Chan, Simon N. Powell, Paul C. Boutros, John L. Humm, Amita Shukla-Dave, David Pfister, Jorge S. Reis-Filho, Nancy Lee
Summary: Functional imaging using PET to assess tumor hypoxia can guide safe de-escalation of radiotherapy dose to 30Gy, leading to reduced toxicity and improved treatment outcomes. Molecular determinants of response, such as DNA repair defects, can be identified through advanced sequencing techniques and may play a key role in predicting patient response to treatment. Personalized chemoradiotherapy based on intratreatment imaging holds promise for significant reduction in radiotherapy intensity.
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2021)
Meeting Abstract
Oncology
Elizabeth Jeans, Michael Grams, Randi Finley, William Breen, Dawn Owen, Sean Park, Bradley Stish, Scott Lester, Ivy Petersen, Safia Ahmed, Kimberly Corbin, Mark Waddle, Michael Haddock, Kenneth Merrell, Christopher Hallemeier, Nadia Laack, Daniel Ma
AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS
(2021)