Journal
MOVEMENT DISORDERS
Volume 34, Issue 11, Pages 1699-1710Publisher
WILEY
DOI: 10.1002/mds.27810
Keywords
dopamine neurons; genetic models; nonmotor symptoms; prion-like propagation
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Funding
- Swedish Governmental Funding for Clinical Research
- Swedish Research Council
- Basal Ganglia Disorders Linnaeus consortium
- Multidisciplinary Research on Parkinson's Disease
- Wenner-Gren foundations
- Ahlen Foundation
- Dementiafonden
- Parkinsonfonden
- Sergerfalk Foundation
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Background Cortical alpha-synuclein pathology plays a role in the development of cognitive dysfunction in both Parkinson's disease and dementia with Lewy bodies, although the causative cellular lesions have remained unclear. We aimed to address causal links between alpha-synuclein-driven pathology in the cerebral cortex and the development of cognitive impairments using new experimental models. Methods Neuronal overexpression of human alpha-synuclein was induced in the rat medial prefrontal cortex using viral vectors. This was combined with inoculations of preformed fibrils of human alpha-synuclein in some animals. Rats were evaluated with tests probing prefrontal cognitive functions (delayed matching/nonmatching to position and 5-choice serial reaction time task). Patterns of neuropathology were characterized immunohistochemically. Results Neither alpha-synuclein overexpression nor the fibril seeds alone yielded any behavioral phenotype. In contrast, combining the 2 approaches produced significant impairments in working memory, attention, and inhibitory control. All animals injected with alpha-synuclein vectors exhibited high immunoreactivity for human alpha-synuclein in the medial prefrontal cortex and its primary projection targets. However, only when this overexpression was combined with fibril inoculations did animals exhibit large, proteinase K-resistant and Ser(129)-phosphorylated alpha-synuclein intraneuronal inclusions in the medial prefrontal cortex and its closely interconnected brain regions. The inclusions were associated with distorted dendritic morphologies and partial neuronal loss in the targeted cortical areas. Conclusions Cortical overexpression of human alpha-synuclein is not sufficient to produce cognitive dysfunction, whereas combining this overexpression with fibril seeds yields both cognitive and histopathological phenotypes that are relevant to human Lewy body disease. (c) 2019 International Parkinson and Movement Disorder Society
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