Journal
JOURNAL OF SEPARATION SCIENCE
Volume 42, Issue 20, Pages 3182-3190Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201900252
Keywords
Corydalis impatiens; isoquinoline alkaloids; preparative separation; two-dimensional liquid chromatography
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Funding
- Applied Basic Research Project of Qinghai Province [2016-ZJ-714]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017471]
- International Cooperation Project of Qinghai Province [2019-HZ-805]
- Instrument Function Development Project of the Chinese Academy of Sciences [2019gl11]
- Innovation Platform for the Development and Construction of Special Project of Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province [2017-ZJ-Y19]
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We established a two-dimensional strong cation exchange/reversed-phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium-pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22-29 for reversed-phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22-29) were further separated, and those of low resolution were isolated via two-dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off-line two-dimensional strong cation exchange/reversed-phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.
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