Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 70, Issue 21, Pages 6127-6139Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erz363
Keywords
Coevolution; duplication; gene copies; homology modelling; molecular evolution; multigene family; photosynthesis; positive selection; rbcS; RuBisCO
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Funding
- Swiss National Science Foundation [31003A_138282]
- LABEX TULIP [ANR-10-LABX-0041]
- LABEX CEBA [ANR-10-LABX-25-01]
- Swiss National Science Foundation (SNF) [31003A_138282] Funding Source: Swiss National Science Foundation (SNF)
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Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is considered to be the main enzyme determining the rate of photosynthesis. The small subunit of the protein, encoded by the rbcS gene, has been shown to influence the catalytic efficiency, CO2 specificity, assembly, activity, and stability of RuBisCO. However, the evolution of the rbcS gene remains poorly studied. We inferred the phylogenetic tree of the rbcS gene in angiosperms using the nucleotide sequences and found that it is composed of two lineages that may have existed before the divergence of land plants. Although almost all species sampled carry at least one copy of lineage 1, genes of lineage 2 were lost in most angiosperm species. We found the specific residues that have undergone positive selection during the evolution of the rbcS gene. We detected intensive coevolution between each rbcS gene copy and the rbcL gene encoding the large subunit of RuBisCO. We tested the role played by each rbcS gene copy on the stability of the RuBisCO protein through homology modelling. Our results showed that this evolutionary constraint could limit the level of divergence seen in the rbcS gene, which leads to the similarity among the rbcS gene copies of lineage 1 within species.
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