Article
Environmental Sciences
Mary Jean See, David C. Bencic, Robert W. Flick, Jim Lazorchak, Adam D. Biales
Summary: This study characterized the VTG protein in male fathead minnow mucus compared to plasma and liver mRNA. The results showed that the induction kinetics of VTG protein concentration in mucus and plasma were similar, with a significant increase detected after 2 days of exposure in the mucus-based assay and after 7 days in the plasma-based assay.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Ju Mu, Yu Wang, Miao Wang, Dayi Zhang, Mingying Liu
Summary: This study constructed a mouse model exposed to poly-propylene microplastics (PP-MPs) to evaluate the bioaccumulation of PP-MPs in the kidney and explore potential reference genes using RT-qPCR. The stability and reliability of 19 candidate reference genes were assessed using four algorithms, and the top five most stable genes were determined (Cox4i1, Rps3, Rps9, Rps18, and Gapdh). Three genes associated with different biochemical processes were chosen to verify the identified reference genes, showing different expression profiles and emphasizing the importance of selecting reliable reference genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Karan Parekh, Vicki L. Marlatt
Summary: This study investigated the effects of Metro Vancouver's wastewater treatment plant effluents on hepatic gene expression in English sole. The results showed differences in xenobiotic defense, thyroid function, lipid and glucose metabolism, protein synthesis, and reproduction at impacted sites. Male English sole exhibited widespread exposure to estrogenic contaminants, while the induction of hepatic CYP1A was observed due to influx of certain hydrocarbons from industrial contributors.
Article
Plant Sciences
Qizhang Wang, Chunqian Guo, Shipeng Yang, Qiwen Zhong, Jie Tian
Summary: The aim of this study was to identify suitable reference genes for gene expression analysis during different growth conditions in garlic. Nine candidate reference genes were selected using garlic transcriptome sequence data and their expression levels were evaluated in specific tissues under drought and cold stress. Several statistical methods were used to assess the stability of the reference gene expression levels. The most stable reference genes were identified, and their reliability was confirmed by evaluating the expression of a stress-responsive gene. This study provides a theoretical reference for gene expression analysis in garlic under stress conditions.
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Fisheries
Joanna Szczygiel, Teresa Kaminska-Gibas, Jules Petit, Patrycja Jurecka, Geert Wiegertjes, Ilgiz Irnazarow
Summary: The study ranked the expression stability of duplicated genes in different tissues of common carp and found that 40s, b2m, ef1 alpha, and actb genes showed the most stable expression across all physiological conditions, while gapdh, odc1, and g6pd genes demonstrated lower stability. Functional divergence between duplicated genes was observed, emphasizing the importance of considering this aspect in selecting reference genes in polyploid species.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Plant Sciences
Julianna Matos da Silva Soares, Anelita de Jesus Rocha, Fernanda dos Santos Nascimento, Vanusia Batista Oliveira de Amorim, Andresa Priscila de Souza Ramos, Claudia Fortes Ferreira, Fernando Haddad, Edson Perito Amorim
Summary: This study investigated the interaction between Musa sp. and P. fijiensis in banana plants and identified differentially expressed genes related to defense mechanisms against black Sigatoka. The findings showed the activation of phenolic compounds and callosis as defense mechanisms in resistant genotypes. The resistant genotype Krasan Saichon stood out and has potential for breeding programs for resistance to black Sigatoka in bananas.
Article
Oncology
Beatriz Sanchez-Sendra, Silvia Perez-Deben, Jose F. Gonzalez-Munoz, Amelia Murgui, Carlos Monteagudo
Summary: The expression of IGF2BP3 mRNA in melanoma is closely related to the risk of metastasis and survival, with RT-qPCR being more reliable than immunohistochemistry for assessment. It can be used to evaluate patient prognosis and individualized treatment.
Article
Dentistry, Oral Surgery & Medicine
Manuel Toledano-Osorio, Francisco Javier Manzano-Moreno, Manuel Toledano, Raquel Osorio, Antonio L. Medina-Castillo, Victor J. Costela-Ruiz, Concepcion Ruiz
Summary: The study investigated the impact of novel silicon dioxide composite membranes functionalized with zinc or doxycycline on osteoblastic cell gene expression. Results showed that the use of SiO2-NPs in polymeric scaffolds improved osteogenic gene expression, with the best results achieved by doxycycline doping. Zinc and doxycycline functionalized membranes significantly influenced the expression of several osteogenic gene markers, highlighting their potential for use in GBR procedures.
JOURNAL OF DENTISTRY
(2021)
Article
Biochemistry & Molecular Biology
Chaofan Jiang, Lin Zhou, Qingchen Zhao, Mengke Wang, Sirui Shen, Te Zhao, Kaidi Cui, Leiming He
Summary: This study validated the expression stability of seven candidate reference genes in Sclerotium rolfsii under different conditions and recommended suitable reference gene combinations for RT-qPCR normalization. The results showed that using one or two reference genes to normalize the expression of target genes in S. rolfsii did not significantly differ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Review
Chemistry, Analytical
Sulaiman Alsalameh, Khalid Alnajjar, Tariq Makhzoum, Noor Al Eman, Ismail Shakir, Tanveer Ahmad Mir, Khaled Alkattan, Raja Chinnappan, Ahmed Yaqinuddin
Summary: This article reviews the recent advances and advantages of using biosensors for COVID-19 diagnosis. Traditional methods have limitations, while biosensors are sensitive, specific, rapid, and user-friendly, with the potential to go beyond traditional standards in diagnostics.
Review
Oncology
Yue Zhang, Xinyi Zhang, Yumeng Xu, Shikun Fang, Ying Ji, Ling Lu, Wenrong Xu, Hui Qian, Zhao Feng Liang
Summary: This review provides a detailed overview of the classification, characteristics, biogenesis, functions, and latest advances in cancer research of circular RNAs (circRNAs). The unique features of circRNAs, such as their high abundance, strong specificity, and good stability, have been identified, and their various functions and mechanisms in biological events, including cancer occurrence and development, have been extensively studied. The development of circRNAs as biomarkers in cancer diagnosis and treatment also opens up new avenues for circRNA research.
FRONTIERS IN ONCOLOGY
(2022)
Article
Genetics & Heredity
Dongxiao Ma, Qin Zhang, Jintao Zhou, Yu Lu, Xiaomeng Duan, Chengzhong He, Jinde Yu
Summary: In this study, the expression stability of 10 candidate reference genes was analyzed in Toxicodendron succedaneum using RT-qPCR. The results showed that TsRG03 and ACT were recommended as suitable reference genes for all samples. These findings provide alternative reference genes for gene function studies, correction and normalization of target gene expression, and directed molecular breeding in T. succedaneum.