Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 67, Issue 37, Pages 10534-10542Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b04996
Keywords
fucosylated chondroitin sulfate; Stichopus japonicus; digestion; pancreatic lipase; alpha-amylase
Funding
- National Natural Science Foundation of China [31972084, 31872903, 31771998]
- National Key R&D Program of China [2018YFD0901000, 2017YFD0400203]
- Dalian High-level Talents Innovation and Entrepreneurship Project [2016RQ061]
- Guangxi University Strong Foundation Program-Graduate Overseas Training Program
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Fucosylated chondroitin sulfate from sea cucumber Stichopus japonicus (FCSSJ) has been demonstrated with various biological activities; however, its precise structure is still controversial, and digestive behavior remains poorly understood. FCSSJ was purified, and its detailed structure was elucidated mainly based on the NMR spectroscopic methods. Its main chain was characterized as -> 4)-beta-D-GlcA-(1 -> 3)-beta-D-GalNAc-(1 -> with GalNAc4S6S:GalNAc4S in a ratio of 1.5:1, and three types of sulfated fucosyl branches attaching C-3 of GlcA, namely, Fucp2S4S, Fucp3S4S, and Fucp4S, were found in a ratio of 2:1.5:1. The digestibility of FCSSJ was investigated in vitro, and the unchanged molecular weight and reducing sugar content indicated that FCSSJ was not broken down under salivary and gastrointestinal digestion. Furthermore, FCSSJ showed a significant inhibitory impact on pancreatic lipase dose-dependently but not on alpha-amylase, indicating that the inhibition of pancreatic lipase by FCSSJ might be a pathway for its hypolipidemic effect. These findings propose a fucosylated chondroitin sulfate and provide insight into the mechanism of its physiological effects in the digestion system.
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