Article
Fisheries
E. S. Kladchenko, T. A. Kukhareva, V. N. Rychkova, A. A. Soldatov, E. S. Chelebieva, A. Y. Andreyeva
Summary: Bivalve mollusks such as the ark clam are unable to maintain a constant internal osmolarity in conditions of salinity stress. In this study, the effect of hypersalinity stress on the cellular composition and morphology of the ark clam's hemocytes was investigated. The results showed that exposure to hypersalinity induced changes in erythrocyte morphology and an increase in hemocyte diameter. However, hyperosmotic stress did not affect hemocyte viability or hemolymph cellular composition.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Food Science & Technology
Ga-Young Lee, Eiseul Kim, Seung-Min Yang, Hae-Yeong Kim
Summary: A rapid on-site identification method based on a microfluidic chip was developed for the detection of three ark shell species. New species-specific primers were designed and their specificity and sensitivity were evaluated. A direct ultrafast PCR method was successfully developed for on-site identification and monitoring of the species in commercial food products.
Article
Biochemical Research Methods
Yiming Wang, Xiaojie Wang, Tingrui Pan, Baoqing Li, Jiaru Chu
Summary: This technology utilizes real-time cellular recognition and microfluidic impact printing to efficiently isolate single cells in a label-free manner, with high efficiency and survival rate demonstrated in experiments. It has significant potential in various fields including single-cell omics, tissue engineering, and cell-line development.
Article
Zoology
Ekaterina S. Kladchenko, Aleksandra Y. Andreyeva, Igor Mindukshev, Stepan Gambaryan
Summary: This study used laser diffraction method to analyze the volume changes in Anadara kagoshimensis hemocytes following hyposmotic stimulation. The results showed that hemocytes of Anadara kagoshimensis rapidly swelled and then recovered their volume after hyposmotic stimulation.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
E. S. Kladchenko, O. L. Gostyukhina, A. A. Soldatov, V. N. Rychkova, A. Yu. Andreyeva
Summary: This study analyzed the antioxidant enzymatic activities and functional state of hemocytes in ark clams exposed to salinity stress. The results showed that ark clams have tolerance to hypersalinity stress and sensitivity to hyposalinity treatment. High salinity increased mitochondrial membrane potential and restored intracellular ROS levels, while low salinity increased ROS and mitochondrial membrane potential levels. Acclimation to low and high salinity did not affect osmotic fragility of hemocytes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Grace Afumwaa Boamah, Zekun Huang, Yawei Shen, Yisha Lu, Zhixuan Wang, Ying Su, Changan Xu, Xuan Luo, Caihuan Ke, Weiwei You
Summary: This study investigated the molecular and cellular mechanisms of low salinity adaptation in abalone by analyzing the transcriptome and flow cytometry data. The results showed that abalone up-regulated calmodulin and heat-shock protein, and down-regulated tumor necrosis factor and nuclear factor kappa B in response to low salinity. The hybrid abalone exhibited higher survival rate and more active immune response, suggesting that hybridization could be a method to breed stress-resilient aquatic species.
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Biotechnology & Applied Microbiology
Thalita Massaro Malheiros Ferreira, Jaire Alves Ferreira Filho, Andre Pereira Leao, Carlos Antonio Ferreira de Sousa, Manoel Teixeira Souza
Summary: This study aimed to select, annotate, and validate stress-inducible genes differentially expressed in the leaves of oil palm plants under saline stress. Through transcriptome analysis and validation using qRT-PCR, 14 genes were selected, annotated, and their expression profiles were examined. The analysis revealed that some of these genes are orthologs of salt resistance genes in other species, coding for proteins involved in salt transport, transcriptional regulation, and stomata opening and closing. The study also identified various cis-acting elements in the promoter sequences of these genes, some of which are known to be involved in abiotic stress. The results provide valuable information for gene selection, promoter sequence analysis, and molecular-genetics-assisted breeding programs.
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Chang-Hun Choi, Eiseul Kim, Seung-Min Yang, Da-Som Kim, Seung-Man Suh, Ga-Young Lee, Hae-Yeong Kim
Summary: This study evaluated the applicability of a ddPCR assay for detecting lactic acid bacteria and compared its performance characteristics to those of a qPCR. The results showed that ddPCR is more sensitive than qPCR, but it has limitations in quantifying high bacterial concentrations.
Article
Pathology
Shu Jin Tan, Todd Matthew Pryce, Erin Judith Haygarth, Peter Alan Boan
Summary: The study demonstrated the utility of a Pneumocystis jirovecii quantitative polymerase chain reaction (qPCR) in distinguishing disease from colonization, with varying sensitivity and specificity in HIV and non-HIV patients. Standardization of methods and definitions is necessary for improving generalizability of results.