Pregnancy history and blood-borne microvesicles in middle aged women with and without coronary artery calcification

Background and aims: Having a history of preeclampsia increases the risk for future coronary artery calcification (CAC). This study evaluated the association of blood-borne, cell-derived microvesicles (MV) with CAC in middle-aged women. Methods: Twelve pre-selected, antigen-specific MV were measured by digital flow cytometry in the blood of age-and parity-matched women (median age 60 years) without a history of cardiovascular events, but with either a history of preeclampsia (PE, n-39) or normotensive pregnancy (NP, n-40). CAC was determined by computed tomography. Results: CAC scores ranged from 0 to 47 and 0-602 Agatston Units in the NP and PE groups, respectively. Waist circumference and insulin resistance were greatest in PE women with CAC. MV positive for tissue factor or stem/progenitor cell antigen (CD117) differed between NP and PE groups. In univariate analysis, those positive for tissue factor, ICAM-1, stem cells, and adipocytes (P16-set) antigens associated with CAC in the PE group. Principal components (PC) analysis reduced the MV variables to three independent dimensions. PC1 showed a modest correlation with CAC scores in the PE group (rho = 0.31, p = 0.06) and associated with CAC in a multivariable model on pooled groups that included all 3 PC variables when adjusted for pregnancy status (p = 0.03). The association was lost when corrected for body mass index or waist circumference. Conclusions: In women with a history of PE and elevated metabolic risk profile, a group of specific antigen-positive MV associated with CAC. These MV may reflect cellular processes associated with CAC. Their diagnostic potential for CAC remains to be determined. (C) 2016 Elsevier Ireland Ltd. All rights reserved.