Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 136, Issue -, Pages 547-558Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.06.080
Keywords
Exopolysaccharide; Characterization; Gold nanoparticles; Antioxidant activity; Natronotalea sambharensis
Funding
- Council of Scientific and Industrial Research, India
- Department of Science and Technology, Government of India
- Process development for production of microbial exopolysaccharides [OLP 0854]
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An exopolysaccharide producing strain AK103(T) was isolated from Sambharlake, Rajasthan, India. Based on poly phasic taxonomy, the strain AK103(T) was found to be a novel species for which the name Natronotalea sambharensis sp. nov. is proposed. The strain was able to secrete (1.2 gL(-1)) EPS under optimum conditions. The FT-IR spectroscopy revealed that EPS had carboxyl, hydroxyl, carbonyl and sulfate ester groups. The high performance liquid chromatography (HPLC), gel permeation chromatography (GPC) and NMR revealed that the exopolysaccharide was composed of mannose, glucose and glucuronic acid with a molecular weight of 4.6 x 10(6) KDa. This novel EPS was designated as Nat103. Interestingly, the EPS was found to have antioxidant activity and in vitro antioxidant studies showed that DPPH (2,2-diphenyl-1-picrylhydrazyl) activity increased in a dose dependent manner. Furthermore, EPS Nat103 was able to produce gold nanoparticles which were further characterized by FT-IR, DLS and Zeta potential (ZP). (C) 2019 Elsevier B.V. All rights reserved.
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