Article
Microbiology
Shengnan Jiang, Mengzhen Chen, Junxiong Zhang, Xiaoliang Ba, Hao Zhang, Yueqin Hong, Lu Sun, Zhengan Wang, Hemu Zhuang, Feiteng Zhu, Yiyi Chen, Haiping Wang, Feng Zhao, Yan Chen, Yunsong Yu, Shujuan Ji
Summary: Our study found a wide distribution of DAP resistance and heteroresistance in MRSA in China. The resistance to DAP in MRSA may threaten its effectiveness against MRSA infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Andreas F. Wendel, Robin Otchwemah, Franziska Layer-Nicolaou, Frauke Mattner, Carlos J. Tellez-Castillo, Robert Skov, Henrik Oberlaender, Guido Werner, Birgit Strommenger
Summary: This study investigated the causes of the emergence and spread of daptomycin-resistant Staphylococcus aureus (DAP-R) in three medical centers in Cologne, Germany. It was found that the acquisition of DAP resistance mainly occurred in patients with wounds who were treated with antiseptic rather than systemic antibiotic therapy. The study also demonstrated that treatment with polyhexamethylene biguanide/polyhexanide (PHMB) could lead to the development of DAP resistance.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
Aurelie Guyet, Amirah Alofi, Richard A. A. Daniel
Summary: Gram-positive bacterial cells are protected by a cell envelope comprised of peptidoglycan and teichoic acid polymers. The loss of peptidoglycan synthesis proteins leads to cell death and alters the lipoteichoic acids and autolysin LytE. The enzyme MprF is found to have a role in modulating LTA biosynthesis.
Article
Infectious Diseases
Carla C. C. R. de Carvalho, Agustina Taglialegna, Adriana E. Rosato
Summary: The research findings indicate that PrsA plays a key role in cell membrane remodeling related to the see-saw effect, and may have a significant role in the resensitization of daptomycin-resistant strains to beta-lactams, such as oxacillin, in addition to its post-transcriptional role in the maturation of PBP 2a.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Masaki Nakamura, Hayato Kawada, Hiroki Uchida, Yusuke Takagi, Shuichi Obata, Ryotaro Eda, Hideaki Hanaki, Hidero Kitasato
Summary: The study identified that the T345I SNP in the mprF gene may be one of the primary causes of DAP-non-susceptible MRSA strains.
Article
Immunology
Rutan Zhang, Hari Polenakovik, Ismael A. Barreras Beltran, Adam Waalkes, Stephen J. Salipante, Libin Xu, Brian J. Werth
Summary: This study examines the treatment of MRSA infection in a patient with end-stage renal disease. The patient developed resistance to vancomycin, daptomycin, and dalbavancin, which was associated with genetic mutations, reduced lipid content, and reduced membrane fluidity.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Christopher R. Miller, Jonathan M. Monk, Richard Szubin, Andrew D. Berti
Summary: Understanding the impact of antibiotic tolerance on subsequent resistance development is crucial for effective therapeutic interventions. This study highlights the rapid development of resistance in a patient with MRSA bacteremia, identifying genetic and metabolic changes associated with this evolution. The patient's isolates showed increased tolerance to daptomycin, contributing to the subsequent emergence of resistance.
Article
Infectious Diseases
Sabrina Di Gregorio, Maria Sol Haim, Angela Maria Rosa Famiglietti, Jose Di Conza, Marta Mollerach
Summary: This study characterized isogenic strains of S. aureus with vancomycin resistance, both in vivo and in vitro, and found genetic changes associated with cell wall and phosphate metabolism, staphylococcal growth, and biofilm formation. These changes are not commonly associated with vancomycin resistance in S. aureus.
Article
Microbiology
Shengnan Jiang, Hemu Zhuang, Feiteng Zhu, Xiang Wei, Junxiong Zhang, Lu Sun, Shujuan Ji, Haiping Wang, Dandan Wu, Feng Zhao, Rushuang Yan, Yunsong Yu, Yan Chen
Summary: The emergence of daptomycin-resistant Staphylococcus aureus strains is a global problem caused by point mutations in the mprF gene. This study found that specific mprF mutations lead to the seesaw effect of distinct beta-lactams in MRSA strains and the combination therapy of daptomycin and beta-lactams has synergistic activity. The cell surface positive charge is not affected by mprF mutations. Understanding the mechanism of restoring susceptibility to beta-lactam antibiotics mediated by mprF mutations is important for the treatment of MRSA infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Cameron P. Gill, Christopher Phan, Vivien Platt, Danielle Worrell, Thomas Andl, Herve Roy
Summary: Lysyl-diacylglycerol (Lys-DAG) is a novel molecular determinant for antimicrobial resistance and virulence, conserved in most Actinobacteria, and contributes to overall membrane physiology in this important bacterial phylum.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Brian J. Werth, Nathaniel K. Ashford, Kelsi Penewit, Adam Waalkes, Elizabeth A. Holmes, Dylan H. Ross, Tianwei Shen, Kelly M. Hines, Stephen J. Salipante, Libin Xu
Summary: Dalbavancin showed bactericidal activity against most strains of MRSA in vitro, but also led to the development of resistance to dalbavancin, vancomycin, and daptomycin while increasing susceptibility to beta-lactam antibiotics.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Microbiology
Amy G. Prater, Heer H. Mehta, Kathryn Beabout, Adeline Supandy, William R. Miller, Truc T. Tran, Cesar A. Arias, Yousif Shamoo
Summary: The LiaFSR signaling pathway plays a crucial role in mediating daptomycin resistance in enterococci, as the lack of a functional pathway can lead to hypersusceptibility. Knocking out LiaFSR signaling significantly delays the onset of resistance, but eventually resistance can emerge through various alternative mechanisms influenced by the environment.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Weiwei Li, Jiamin Hu, Ling Li, Mengge Zhang, Qingyu Cui, Yanan Ma, Hainan Su, Xuhua Zhang, Hai Xu, Mingyu Wang
Summary: This study identified a multidrug-resistant Enterococcus faecium strain EF332 that is resistant to both daptomycin and vancomycin, and discovered new genetic mutations that confer daptomycin resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Li Liu, Li Ji, Yong Lai, Lili Yang, Gongxiu He, Dangquan Zhang
Summary: The essential oils from twigs and leaves of P. bournei are natural antibacterial agents with strong inhibition against Bacillus cereus and Staphylococcus aureus. The main components of P. bournei essential oils, sesquiterpenes, including (+)- & delta;-Cadinene, Caryophyllene, and Germacrene D, have potential key components inhibiting the survival of B. cereus and S. aureus.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Microbiology
Adeline Supandy, Heer H. Mehta, Truc T. Tran, William R. Miller, Rutan Zhang, Libin Xu, Cesar A. Arias, Yousif Shamoo
Summary: Infections caused by vancomycin-resistant Enterococcus faecium (VREfm) are a significant public health threat. The study investigates the potential for VREfm to develop resistance to the combination therapy of daptomycin (DAP) and fosfomycin (FOS), and whether there are genetic drivers that may decrease the efficacy of the combination or delay the onset of resistance. The results show that the combination therapy can effectively inhibit VREfm without substantial cross-drug epistasis, and the addition of FOS can prolong the efficacy of DAP and slow down DAP resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Ying Wang, Hua Gao, Fudi Wang, Zhongde Ye, Michal Mokry, Adam W. Turner, Jianqin Ye, Simon Koplev, Lingfeng Luo, Tom Alsaigh, Shaunak S. Adkar, Maria Elishaev, Xiangyu Gao, Lars Maegdefessel, Johan L. M. Bjorkegren, Gerard Pasterkamp, Clint L. Miller, Elsie G. Ross, Nicholas J. Leeper
Summary: This study reveals that vascular smooth muscle cells (SMCs) undergo dedifferentiation and activation of pro-inflammatory pathways during the development of atherosclerosis. ATF3, a transcription factor, is identified as an upstream regulator of this transition. ATF3 represses the transition of SMCs towards a subset of cells that promote vascular inflammation by activating the complement cascade. The expression of ATF3 is negatively correlated with complement component C3, and genetic variations that reduce ATF3 expression are associated with an increased risk for atherosclerosis.
CARDIOVASCULAR RESEARCH
(2022)
Article
Infectious Diseases
Shijun Sun, Qi Wang, Longyang Jin, Yifan Guo, Yuyao Yin, Ruobing Wang, Lei Bi, Renfei Zhang, Yungang Han, Hui Wang
Summary: This study identified novel subtypes and the spread of tmexCD-toprJ gene clusters. The research revealed that these gene clusters are mainly distributed in Pseudomonas spp. and Klebsiella spp. and can be transferred between Enterobacterales through plasmids.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Yawei Zhang, Xiaojuan Wang, Shifu Wang, Shijun Sun, Henan Li, Hongbin Chen, Qi Wang, Hui Wang
Summary: This study revealed the association between colistin resistance and tigecycline pressure in CR-hvKP through clinical cases and in vitro experiments. It was found that developing colistin resistance under tigecycline or colistin pressure may result in a slight virulence cost for CR-hvKP, although the mutants still displayed higher virulence compared to the parent strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hua Gao, Richard A. Baylis, Lingfeng Luo, Yoko Kojima, Caitlin F. Bell, Elsie G. Ross, Fudi Wang, Nicholas J. Leeper
Article
Multidisciplinary Sciences
Mislav Acman, Ruobing Wang, Lucy van Dorp, Liam P. Shaw, Qi Wang, Nina Luhmann, Yuyao Yin, Shijun Sun, Hongbin Chen, Hui Wang, Francois Balloux
Summary: Acman et al. investigate the dynamics of bla(NDM) dissemination across the globe and identify the mobile genetic elements and specific mobilization events involved in the gene's global spread.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Qi Wang, Longyang Jin, Shijun Sun, Yuyao Yin, Ruobing Wang, Fengning Chen, Xiaojuan Wang, Yawei Zhang, Jun Hou, Yumei Zhang, Zhijie Zhang, Liuchun Luo, Zhusheng Guo, Zhenpeng Li, Xin Lin, Lei Bi, Hui Wang
Summary: Cefiderocol, a new siderophore cephalosporin, has been approved in the United States and Europe but not in China. There is limited data on the antimicrobial susceptibility of cefiderocol in China. This study evaluated the susceptibility of carbapenem-resistant Enterobacterales (CRE) to cefiderocol in China and investigated the resistance mechanism. Cefiderocol showed activity against most CRE strains, but a high proportion of Escherichia coli showed high-level resistance. The resistance mechanism was found to involve the premature stop codon of cirA, the existence of bla(NDM-5), and the mutation of pbp3.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yue Gao, Henan Li, Hongbin Chen, Jiangang Zhang, Ruobing Wang, Zhiren Wang, Hui Wang
Summary: This study conducted a comprehensive genomic analysis of 456 clinical strains of ST208 A. baumannii from a wide temporal and geographic range, with a focus on the horizontal transfer mechanisms of bla(OXA-23). The study described experimental evidence of the potential circular intermediate of Tn2009 and detected for the first time the tandem structure of five copies of bla(OXA-23) in Tn2009. The interbacterial transfer of genetic elements carrying resistance to last-line antibiotic carbapenems highlights the essential need to enhance epidemiologic surveillance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Shuyi Wang, Qi Ding, Yawei Zhang, Anru Zhang, Qi Wang, Ruobing Wang, Xiaojuan Wang, Longyang Jin, Shuai Ma, Hui Wang
Summary: The evolution of the capsule may lead to the convergence of carbapenem resistance and hypervirulence in Klebsiella pneumoniae. Inactivation of the capsule gene wcaJ may compromise the virulence of the strain, but isolates with wcaJ interruption demonstrated a lower fitness cost and a high conjugation frequency of the blaKPC-2 plasmid, raising concerns about the emergence of carbapenem resistance in hypervirulent K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Qiaoyan Duan, Qi Wang, Shijun Sun, Qiaozhen Cui, Qi Ding, Ruobing Wang, Hui Wang
Summary: The nosocomial spread of carbapenem-resistant Enterobacterales (CRE) is common and places severe burdens on healthcare systems. The high-risk Klebsiella pneumoniae ST11 strain has a wide endemic area in China. This study monitored CRE genotypes and phenotypes in a tertiary hospital in North China from 2012 to 2020. The results showed that the ST11 strain carrying bla(NDM) exhibited strong adaptability and could potentially replace other strains to become an epidemic strain. Infection control and preventive measures should focus on the high-risk ST11-K. pneumoniae strain.
Article
Microbiology
Fengning Chen, Yuyao Yin, Hongbin Chen, Longyang Jin, Shuguang Li, Ruobing Wang, Shuyi Wang, Qi Wang, Shijun Sun, Hui Wang
Summary: This study analyzed 142 strains of Staphylococcus aureus and found that the ST59 and ST398 lineages have higher virulence and proteolytic activity compared to other lineages. They also had stronger recognition by human neutrophils and caused more cell apoptosis and neutrophil extracellular trap degradation. Additionally, these strains showed differences in their adhesion genes and adaptability to human epidermal keratinocytes.
Review
Infectious Diseases
Allison M. Hitchcock, Wesley D. Kufel, Keri A. Mastro Dwyer, Eric F. Sidman
Summary: Lenacapavir is a novel HIV-1 treatment option for patients with multidrug-resistant (MDR) HIV-1 infection. It has a favorable pharmacokinetic profile and has shown good tolerability and efficacy in clinical trials.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
Article
Infectious Diseases
Roberta Gagliardini, Alessandro Tavelli, Stefano Rusconi, Sergio Lo Caputo, Vincenzo Spagnuolo, Maria Mercedes Santoro, Andrea Costantini, Alessandra Vergori, Franco Maggiolo, Andrea Giacomelli, Giulia Burastero, Giordano Madeddu, Eugenia Quiros Roldan, Antonella d'Arminio Monforte, Andrea Antinori, Alessandro Cozzi-Lepri
Summary: This study evaluated multiple treatment failures to modern antiretroviral therapy in HIV-infected individuals and found that approximately 4% of them were difficult to treat. The difficult to treat group, compared to the non-difficult to treat group, was characterized by older age, higher prevalence of AIDS, lower CD4+ cell count, and higher risk of treatment failure.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)