Review
Cell Biology
Linda Studer, Annika Blank, John-Melle Bokhorst, Iris D. Nagtegaal, Inti Zlobec, Alessandro Lugli, Andreas Fischer, Heather Dawson
Summary: Tumour budding in colorectal cancer serves as a robust and independent biomarker of aggressive tumor biology, with evidence strongly supporting its reporting in routine practice. Establishing a standardized and evidence-based scoring method is crucial for implementing tumour budding assessment, while the development of automated methods for assessment is a key focus for further research and validation to ensure accuracy and standardization.
Article
Pathology
Han-Na Kim, Soo Yeon Lee, Baek-Hui Kim, Chung-Yeul Kim, Aeree Kim, Hayeon Kim
Summary: Tumor budding in gallbladder cancer has clinical and pathological implications, with high TB grades correlating with poorer survival outcomes, but not significantly associated with death or recurrence risk in multivariate analysis.
Article
Pathology
Philipp Lohneis, Lena Hieggelke, Florian Gebauer, Markus Ball, Christiane Bruns, Reinhard Buettner, Heike Loeser, Alexander Quaas
Summary: Tumor budding in esophageal adenocarcinomas, evaluated according to the ITBCC criteria, is an independent prognostic factor associated with reduced overall survival. Regardless of the quantification approach used, an increased number of tumor buds is significantly correlated with worse overall survival. Prospective studies using ITBCC criteria are needed to confirm these findings in the future.
Article
Pathology
Philipp Lohneis, Julia Rohmann, Florian Gebauer, Lena Hieggelke, Christiane Bruns, Wolfgang Schroeder, Reinhard Buettner, Heike Loeser, Alexander Quaas
Summary: This study investigated the role of tumor budding as an additional prognostic factor in esophageal adenocarcinomas with poor response to neoadjuvant therapy, finding a strong positive correlation between high numbers of tumor buds and poor prognosis.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Oncology
Ana Pavlic, Emanuela Bostjancic, Rajko Kavalar, Bojan Ilijevec, Serena Bonin, Fabrizio Zanconati, Nina Zidar
Summary: This study investigates the involvement of epithelial-mesenchymal transition (EMT) in tumor budding (TB) and poorly differentiated clusters (PDCs) in colorectal cancer (CRC). The results suggest that both TB and PDCs are associated with partial EMT, but they exhibit discrete differences in morphology and expression of EMT-related markers.
JOURNAL OF PATHOLOGY
(2022)
Review
Oncology
Nishant Thakur, Muhammad Joan Ailia, Yosep Chong, Ok Ran Shin, Kwangil Yim
Summary: This study evaluated the prognostic significance, clinicopathological value, and relationship with epithelial-mesenchymal transition (EMT) of tumor budding (TB) in lung cancer. The results showed that high-grade TB was significantly associated with poor overall survival (OS) and disease-free survival (DFS), as well as clinicopathological characteristics and EMT markers.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lei Han, Shuyi Wang, Chen Wei, Yan Fang, Sihao Huang, Tailang Yin, Bin Xiong, Chaogang Yang
Summary: Cancer metastasis is closely related to the process of Epithelial-mesenchymal transition (EMT) and the tumor microenvironment (TME), which interact with each other and contribute to the progression of cancer metastasis.
EXPERT REVIEWS IN MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Xiuyuan Zhang, Yiming Li, Pengbo Hu, Liang Xu, Hong Qiu
Summary: In this study, the joint role of epithelial-mesenchymal transition (EMT) and the immune microenvironment in gastric cancer (GC) progression was investigated. A model called EIRG_score was developed to predict prognosis and immunotherapeutic response. The results showed that the EIRG_score was significantly associated with prognosis, immune infiltration, gene mutation, chemotherapeutic drug sensitivity, and immunotherapy response.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Xiangyan Wu, Yuhan Ye, Kenneth J. Vega, Jiannan Yao
Summary: Gastric adenocarcinoma is highly heterogeneous and closely related to colorectal cancer. The existing classification system is not able to identify suitable treatment candidates. This study found that the CMS classification system is efficient for gastric adenocarcinoma, improves clinical outcomes, and identifies candidates who will respond to immunotherapy.
Article
Biotechnology & Applied Microbiology
Jia-Bin Wang, You-Xin Gao, Yin-Hua Ye, Tong-Xing Lin, Ping Li, Jian-Xian Lin, Qi-Yue Chen, Long-Long Cao, Mi Lin, Ru-Hong Tu, Ju-Li Lin, Ze-Ning Huang, Hua-Long Zheng, Jian-Wei Xie, Chao-Hui Zheng, Chang-Ming Huang
Summary: The study demonstrates that CDK5RAP3 plays a tumor suppressor role in gastric cancer and is related to the infiltration and polarization of macrophages. CDK5RAP3 inhibits TAMs polarization to M2 phenotype and promotes M1 polarization. In addition, CDK5RAP3 reduces monocyte recruitment to tumor tissue by inhibiting the CCL2/CCR2 axis.
CANCER GENE THERAPY
(2023)
Article
Oncology
Bernhard Mlecnik, Alessandro Lugli, Gabriela Bindea, Florence Marliot, Carlo Bifulco, Jiun-Kae Jack Lee, Inti Zlobec, Tilman T. Rau, Martin D. Berger, Iris D. Nagtegaal, Elisa Vink-Borger, Arndt Hartmann, Carol Geppert, Julie Kolwelter, Susanne Merkel, Robert Grutzmann, Marc Van den Eynde, Anne Jouret-Mourin, Alex Kartheuser, Daniel Leonard, Christophe Remue, Julia Wang, Prashant Bavi, Michael H. A. Roehrl, Pamela S. Ohashi, Linh T. Nguyen, SeongJun Han, Heather L. MacGregor, Sara Hafezi-Bakhtiari, Bradly G. Wouters, Giuseppe Masucci, Emilia K. Andersson, Eva Zavadova, Michal Vocka, Jan Spacek, Lubos Petruzelka, Bohuslav Konopasek, Pavel Dundr, Helena Skalova, Kristyna Nemejcova, Gerardo Botti, Fabiana Tatangelo, Paolo Delrio, Gennaro Ciliberto, Michele Maio, Luigi Laghi, Fabio Grizzi, Tessa Fredriksen, Benedicte Buttard, Lucie Lafontaine, Pauline Maby, Amine Majdi, Assia Hijazi, Carine El Sissy, Amos Kirilovsky, Anne Berger, Christine Lagorce, Christopher Paustian, Carmen Ballesteros-Merino, Jeroen Dijkstra, Carlijn van de Water, Shannon Van Lent-van Vliet, Nikki Knijn, Ana-Maria Musing, Dragos-Viorel Scripcariu, Boryana Popivanova, Mingli Xu, Tomonobu Fujita, Shoichi Hazama, Nobuaki Suzuki, Hiroaki Nagano, Kiyotaka Okuno, Toshihiko Torigoe, Noriyuki Sato, Tomohisa Furuhata, Ichiro Takemasa, Prabhu Patel, Hemangini H. Vora, Birva Shah, Jayendrakumar B. Patel, Kruti N. Rajvik, Shashank J. Pandya, Shilin N. Shukla, Yili Wang, Guanjun Zhang, Yutaka Kawakami, Francesco M. Marincola, Paolo A. Ascierto, Bernard A. Fox, Franck Pages, Jerome Galon
Summary: This study found that the immune system status is a better predictor of prognosis in early-stage colon cancer patients than tumor cell-related parameters. The consensus Immunoscore accurately identifies early-stage colon cancer patients with different clinical outcomes, regardless of treatment bias.
Article
Biotechnology & Applied Microbiology
Cheng Zhang, Yu Liang, Chun-Dong Zhang, Jun-Peng Pei, Kun-Zhe Wu, Yong-Zhi Li, Dong-Qiu Dai
Summary: LINC01235 is significantly over-expressed in gastric cancer cells and tissues, and its high expression is positively correlated with poor prognosis. It can promote cancer cell metastasis through the EMT pathway.
Review
Medicine, General & Internal
Ting Huang, Hui Bao, Yu-hua Meng, Jian-lin Zhu, Xiao-dong Chu, Xiao-li Chu, Jing-hua Pan
Summary: Breast cancer is a heterogeneous tumor and tumor budding is considered to be an early step in tumor metastasis. However, there are inconsistencies in the evaluation criteria and molecular mechanisms of tumor budding in breast cancer, and further research is needed.
ANNALS OF MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Ming-Yu Chou, Muh-Hwa Yang
Summary: The interplay between EMT and immune metabolic changes in cancer cells during tumor metastasis has gained interest among researchers. Understanding how they influence each other may provide novel explanations for immune surveillance during metastasis. Studying immunometabolism in the regulatory loop between tumor-infiltrating immune cells and EMT is crucial for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Kohei Okuyama, Keiji Suzuki, Souichi Yanamoto
Summary: This article summarizes the latest research on the relevance of tumor budding (TB) and partial epithelial-mesenchymal transition (p-EMT) as prognostic markers in the tumor microenvironment (TME) of head and neck squamous cell carcinoma (HNSCC). Understanding the mechanism of TB onset and the relationship between p-EMTs may facilitate the development of novel diagnostic and prognostic methods, and targeted therapies for the prevention of invasion and metastasis.
Article
Clinical Neurology
Hajrullah Ahmeti, Eva Juettner, Christoph Roecken, Olav Jansen, Matthias Laudes, Michael Synowitz
Summary: Pituitary gland metastases are rare and often diagnosed during autopsies. The most common primary tumors that metastasize to the pituitary gland are breast, lung, thyroid, and renal carcinomas. We present a rare case of spindle cell rhabdomyosarcoma metastasizing to the pituitary gland. The patient experienced symptoms such as headache, visual disorder, panhypopituitarism, and diabetes insipidus. Despite the inability to surgically remove the tumor, biopsy confirmed the diagnosis and the patient received chemotherapy and irradiation. Even high-risk patients can achieve remission after treatment.
JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY
(2023)
Article
Oncology
Magnus Kock Am Brink, Laura Sophie Dunst, Hans-Michael Behrens, Sandra Krueger, Thomas Becker, Christoph Roecken
Summary: The study investigates the problem of intratumoral heterogeneity (ITH) in gastric cancer (GC), and tests the effects of Ki67 and tumor regression on ITH after neoadjuvant/perioperative chemotherapy. The results suggest that Ki67 is significantly associated with tumor regression and may serve as a predictor for chemotherapy sensitivity and an indicator of ITH.
BRITISH JOURNAL OF CANCER
(2023)
Article
Clinical Neurology
Ulf Jensen-Kondering, Nils G. Margraf, Caroline Weiler, Walter Maetzler, Justina Dargvainiene, Kim Falk, Sarah Philippen, Thorsten Bartsch, Charlotte Flueh, Christoph Roecken, Bettina Moeller, Georg Royl, Alexander Neumann, Norbert Brueggemann, Benjamin Roeben, Claudia Schulte, Benjamin Bender, Daniela Berg, Gregor Kuhlenbaeumer
Summary: This study compared the clinical, radiological, and cerebrospinal fluid marker data of patients with CAA, MLH, AD, and healthy controls. The results showed that cSS was more common in CAA, and MLH with cSS was associated with ICH. The concentrations of cerebrospinal fluid markers differed among the groups.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Article
Oncology
Wiebke Siemsen, Christine Halske, Hans-Michael Behrens, Sandra Krueger, Christoph Becker-Pauly, Christoph Roecken
Summary: In this study, the expression of meprin beta in gastric cancer was found to be differentially regulated and correlated with clinicopathological characteristics and patient survival. It may function as either a tumor suppressor or promotor depending on the histological site and context.
Article
Oncology
Dita Ulase, Hans-Michael Behrens, Sandra Krueger, Steffen M. Heckl, Ulrike Ebert, Thomas Becker, Christoph Roecken
Summary: This study analyzed the spatial distribution of LAG3 + cells in gastric cancer and its correlation with clinicopathological and survival data. The results demonstrated significant differences in the spatial distribution of LAG3 + cells and their association with prognosis. The findings highlight the importance of LAG3 as a potential prognostic factor and suggest its role in clinical outcomes and treatment responses.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tim A. Steiert, Genis Parra, Marta Gut, Norbert Arnold, Jean-Remi Trotta, Raul Tonda, Alice Moussy, Zuzana Gerber, Peter M. Abuja, Kurt Zatloukal, Christoph Roecken, Trine Folseraas, Marit M. Grimsrud, Arndt Vogel, Benjamin Goeppert, Stephanie Roessler, Sebastian Hinz, Clemens Schafmayer, Philip Rosenstiel, Jean-Francois Deleuze, Ivo G. Gut, Andre Franke, Michael Forster
Summary: Formalin fixation with paraffin-embedding (FFPE) was developed in the late 19th century and is still commonly used in clinical practice. However, FFPE samples can undergo DNA damage, leading to potential errors in sequencing and analysis. This review discusses mitigation strategies in sample quality control, DNA repair treatments, sample preparation, and bioinformatic analysis of FFPE-DNA.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Bente Holm, Stephan Barsuhn, Hans-Michael Behrens, Sandra Krueger, Christoph Roecken
Summary: This study explored the expression and correlation of RNF43 and LRP1B with clinicopathological characteristics in gastric cancer. The expression of RNF43 and LRP1B was significantly correlated with the Lauren phenotype and with each other. Furthermore, differential effects of RNF43 expression were observed between intestinal and diffuse type gastric cancers. These findings suggest that the expression and biological effects of RNF43 and LRP1B in gastric cancer are context-dependent.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Katharina Pries, Sandra Krueger, Steffen Heckl, Hans-Michael Behrens, Christoph Roecken
Summary: Recent studies have found a connection between certain subunits of the SWI/SNF complex and specific tumor characteristics in gastric cancer. This study aimed to examine the expression patterns of SMARCA4 and SMARCE1, two subunits of the SWI/SNF complex, in gastric cancer and their potential relationship with ERBB2. The results showed significant correlations between the expressions of SMARCA4 and SMARCE1 with ARID1A, p53, and microsatellite status, but not with patient prognosis. Amplification analysis revealed co-amplification of SMARCE1 and ERBB2 in some cases, suggesting potential synergistic effects for improved treatment of gastric cancer.
Article
Biochemistry & Molecular Biology
Florian Richter, Clara Henssen, Tim Alexander Steiert, Tobias Meissner, Anne-Sophie Mehdorn, Christoph Roecken, Andre Franke, Jan-Hendrik Egberts, Thomas Becker, Susanne Sebens, Michael Forster
Summary: Esophageal cancer (EC) has a high mortality rate, and optimizing therapies and dynamically adapting to individuals is crucial. Liquid biopsy is an increasingly important method for residual disease monitoring, but conflicting detection rates and varying levels of circulating tumor DNA (ctDNA) have been observed in previous studies. This study aims to resolve this discrepancy and found that ctDNA in blood can be used for therapy monitoring of EC patients. A combination of solid and liquid samples should be used to guide individualized EC therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Samir Abdullazade, Hans-Michael Behrens, Sandra Krueger, Jochen Haag, Christoph Roecken
Summary: The study validated gene amplification, protein expression, and tumor biological function of MDM2 in gastric cancer, and found a significant correlation between MDM2 amplification and protein expression. However, MDM2 amplification and expression were not associated with patient survival.
Article
Geriatrics & Gerontology
Patricia Huebbe, Stephanie Bilke, Johanna Rueter, Anke Schloesser, Graeme Campbel, Claus-C. Glueer, Ralph Lucius, Christoph Roecken, Andreas Tholey, Gerald Rimbach
Summary: Recent studies have found that the human APOE epsilon 4 allele protects against non-alcoholic fatty liver disease (NAFLD), while epsilon 3 promotes hepatic steatosis and steatohepatitis. In this study, mice expressing the APOE3 or APOE4 protein isoforms were fed a high-fat and high-sucrose diet to induce obesity. The results showed that APOE3 mice had higher liver weights and exhibited more severe hepatic steatosis compared to APOE4 mice. Proteome analysis also revealed differences in proteins related to inflammation, damage response, and lipid storage between APOE3 and APOE4 mice. These findings support the association of increased NAFLD risk with the APOE epsilon 3 allele and suggest potential mechanisms of protection conferred by APOE epsilon 4.
Article
Medicine, Research & Experimental
Hajrullah Ahmeti, Amke Caliebe, Christoph Rocken, Olav Jansen, Maximilian H. Mehdorn, Michael Synowitz
Summary: This study systematically examined 696 patients with primary intracranial meningiomas to assess the effect of preoperative peritumoral brain edema (PTBE) on preoperative symptoms, neurological deficits, postoperative complications, and long-term outcomes. Results showed that PTBE significantly increased the incidences of specific preoperative symptoms, neurological deficits, and postoperative complications. Patients with preoperative PTBE required more medical support after surgery, but all patients had favorable outcomes.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)