Article
Biotechnology & Applied Microbiology
Xiaoyan Zhang, Peng Yu, Haishen Wen, Xin Qi, Yuan Tian, Kaiqiang Zhang, Qiang Fu, Yun Li, Chao Li
Summary: Aquaporin (aqp) proteins are small integral membrane proteins that function as pore channels for water and solute transport. This study identified 17 aqp genes in spotted sea bass and investigated their evolutionary characteristics. Aqp3a may play a crucial role in hypo-osmoregulation, while other aqp genes are also involved in the freshwater acclimation process.
MARINE BIOTECHNOLOGY
(2021)
Article
Fisheries
Yonghang Zhang, Haishen Wen, Yang Liu, Xin Qi, Donglei Sun, Chong Zhang, Kaiqiang Zhang, Meizhao Zhang, Jifang Li, Yun Li
Summary: This study characterized the gill morphological and transcriptomic profiles of spotted sea bass after alkalinity stress, identifying differentially expressed genes and alternative splicing genes related to alkalinity response. Key genes associated with cell-cell junctions, signal transduction, and ion transportation were identified, providing insights into the genetic basis for alkalinity adaptation. The findings of this study will aid in the development of molecular breeding programs for the utilization of alkalinity waters.
Article
Immunology
Zhaosheng Sun, Qian Gao, Youchuan Wei, Zhigang Zhou, Yuxi Chen, Chong Xu, Jiaqi Gao, Danjie Liu
Summary: In this study, four P2X receptor homologues were identified in spotted sea bass and their functions in regulating gene expression related to inflammation were investigated. The results suggest that these receptors may regulate the expressions of pro-inflammatory cytokines via the NF-κB pathway.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Kai Peng, Xiaohui Lv, Hongxia Zhao, Bing Chen, Xiaoying Chen, Wen Huang
Summary: The study found that condensed tannins can protect Lateolabrax maculatus against oxidative damage caused by oxidized fish oil and hydrogen peroxide, improving antioxidant capacity and maintaining intestinal health.
Article
Environmental Sciences
Lingtong Ye, Guangfeng Liu, Tuo Yao, Jie Lu
Summary: The study found that the predominant ARGs in spotted sea bass were floR, sul2, and tetM-01, while sul1, sul2, and cmlA1-01 had significantly higher copy numbers in the environment. There were significant differences in the microbiota of fish gut, gills, and the environment, with Fusobacteria and Actinobacteria potentially acting as hosts for ARGs.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Qing Zhu, Moli Li, Wei Lu, Yapeng Wang, Xujian Li, Jie Cheng
Summary: Salinity and alkalinity are important factors affecting aquatic animals. Chinese sea bass can adapt to diverse salinities but has limited adaptability to highly alkaline water. This study investigated the transcriptomic responses in the gills of Chinese sea bass exposed to salinity change and alkalinity stress. The results revealed a cascade of cellular responses to oxidative and osmotic stress in the gills, with specific responses to alkalinity stress including disrupted immune and antioxidative functions. These findings provide insights for the development of Chinese sea bass cultivation in alkaline water.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Shuya Yuan, Zhaosheng Sun, Qian Gao, Zhen Li, Zhitao Qi, Sidi Zheng, Danjie Liu
Summary: In this study, NLRC3-like, ASC, and Caspase1 genes were identified and characterized in the spotted sea bass. These genes were ubiquitously distributed in various tissues and showed similar expression patterns, with the highest expression in the gill. The results indicate that these genes are involved in the immune response against bacterial infection in the spotted sea bass, providing insight into the immune function and mechanism of the fish inflammasome.
Article
Fisheries
Sishun Zhou, Hao Lin, Lumin Kong, Jianrong Ma, Zhongying Long, Huihui Qin, Zhangfan Huang, Yi Lin, Longhui Liu, Zhongbao Li
Summary: This study investigated the effect of mulberry leaf extract on the liver function of spotted sea bass. The results showed that the extract enhanced the digestion, metabolism, and immunity of the liver, as indicated by increased enzyme activity and gene expression.
AQUACULTURE NUTRITION
(2023)
Article
Fisheries
Shuai Wan, Zhaosheng Sun, Chang Zhang, Tingshuang Pan, Shuya Yuan, Yuxi Chen, Jun Zou, Qian Gao
Summary: The study identified four IL-17 ligands and one receptor gene in spotted sea bass, which play a key role in defense against pathogen-induced infections. The expression of these genes varied in different tissues of the fish. Furthermore, the study revealed that the expression of IL-17 ligands and receptor was modulated by factors such as lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly (I:C)), and Edwardsiella tarda, highlighting their significance in the immune system.
Article
Fisheries
Zhen Wang, Jing Wang, Weijie Mu, Liqun Liang
Summary: Existing data indicate that YB-1, a gene member of the cold chock domain family, is regulated by cold stress in many species. In this study, the expression of LmYB-1 in the spotted sea bass under cold stress was investigated at the transcriptional level. The results showed increased expression of LmYB-1 in liver, brain, and heart tissues, and overexpression of LmYB-1 upregulated the expression of Hsc70, SOD1, and SOD2 in CIK cells.
AQUACULTURE INTERNATIONAL
(2022)
Article
Physiology
Yalong Sun, Haishen Wen, Yuan Tian, Xuebin Mao, Xiurong Li, Junjie Li, Yanbo Hu, Yang Liu, Jifang Li, Yun Li
Summary: This study systematically characterized 5 HSP90 genes and 17 HSP70 genes in spotted seabass, revealing their response mechanisms, evolutionary footprints, and gene structure features in heat stress conditions. The results showed tissue-specific and time-dependent expression patterns of HSP genes in response to heat stress, alkalinity stress, and hypoxia stress, providing essential insights for future functional studies of HSP genes in spotted seabass under abiotic stresses.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bingyu Li, Yuan Tian, Haishen Wen, Xin Qi, Lingyu Wang, Jingru Zhang, Jinku Li, Ximeng Dong, Kaiqiang Zhang, Yun Li
Summary: The study systematically characterized the Sox gene family in spotted sea bass, identifying 26 Sox genes classified into eight subfamilies. The results showed significant expression changes of Sox genes during development, sexually dimorphic patterns during sex differentiation, and unique expression patterns in different adult tissues. These findings provide a fundamental resource for further investigating the functions of Sox genes in various biological processes in spotted sea bass.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Xiangbing Zeng, Hongbiao Dong, Jingru Wu, Wenhao Wang, Yafei Duan, Jian Chen, Jiasong Zhang
Summary: This study investigated the anesthetic potential and physiological response of the essential oil of M. denudata flowers on spotted seabass. The results showed that M. denudata essential oil is an effective anesthetic for spotted seabass.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Immunology
Zhaosheng Sun, Chong Xu, Yuxi Chen, Danjie Liu, Ping Wu, Qian Gao
Summary: This study identifies and characterizes Panx1, Cx32, and Cx43 as important neuro-related immune response genes involved in inflammation-induced ATP release in spotted sea bass.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Fisheries
Jieyun Guo, Lulu Yan, Pengfei Wang, Chao Zhao, Sigang Fan, Lihua Qiu, Yaqing Zhang, Xiuting Qiao
Summary: This study selected 10 candidate reference genes and evaluated their expression stability in different tissues and under different pathogen infection conditions using four algorithms. The results showed that RPL19 was the most stable gene in spotted sea bass under normal conditions, while GAPDH was the most unstable gene. Other stable reference genes were identified in different tissues under various pathogen infections.
AQUACULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Haolong Wang, Haishen Wen, Yun Li, Kaiqiang Zhang, Yang Liu
Article
Biotechnology & Applied Microbiology
Yang Liu, Haolong Wang, Haishen Wen, Yue Shi, Meizhao Zhang, Xin Qi, Kaiqiang Zhang, Qingli Gong, Jifang Li, Feng He, Yanbo Hu, Yun Li
MARINE BIOTECHNOLOGY
(2020)
Article
Biology
Haolong Wang, Timothy J. Bruce, Baofeng Su, Shangjia Li, Rex A. Dunham, Xu Wang
Summary: Hematological assays and transcriptome analysis were performed in the hybrid catfish to investigate the mechanisms of environment-dependent heterosis. The study found that the channel catfish showed lower innate immunity activity, stress, and blood glucose/lactate levels, which are associated with its superior growth performance. Genes involved in fatty acid metabolism/transport pathways were significantly upregulated in channel catfish. The research also revealed that hybrids have increased blood glucose levels due to transgressive expression. This study provides insights into the molecular mechanisms of heterosis and has implications for genetic enhancement through hybrid breeding.
Article
Biochemistry & Molecular Biology
Haolong Wang, Helen R. Montague, Hana N. Hess, Ying Zhang, Gavin L. Aguilar, Rex A. Dunham, Ian A. E. Butts, Xu Wang
Summary: This study investigated the changes in gene expression caused by cryopreservation of catfish sperm. The results showed that cryopreservation significantly increased oxidative stress and DNA fragmentation, and reduced sperm kinematic parameters. The study also found compensatory changes in gene expression after cryopreservation to offset the detrimental effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Haolong Wang, Baofeng Su, Ian A. E. Butts, Rex A. Dunham, Xu Wang
Summary: This study presents the first high-quality assembly of the blue catfish genome, providing important genomic resources and tools for various genetic analyses and gene manipulation techniques.
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)